Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.).
The genetic architecture of plant height was investigated in a set of 358 recent European winter wheat varieties plus 14 spring wheat varieties based on field data in eight environments. Genotyping of diagnostic markers revealed the Rht-D1b mutant allele in 58% of the investigated varieties, while t...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0113287 |
| _version_ | 1819141269579890688 |
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| author | Christine D Zanke Jie Ling Jörg Plieske Sonja Kollers Erhard Ebmeyer Viktor Korzun Odile Argillier Gunther Stiewe Maike Hinze Kerstin Neumann Martin W Ganal Marion S Röder |
| author_facet | Christine D Zanke Jie Ling Jörg Plieske Sonja Kollers Erhard Ebmeyer Viktor Korzun Odile Argillier Gunther Stiewe Maike Hinze Kerstin Neumann Martin W Ganal Marion S Röder |
| author_sort | Christine D Zanke |
| collection | DOAJ |
| description | The genetic architecture of plant height was investigated in a set of 358 recent European winter wheat varieties plus 14 spring wheat varieties based on field data in eight environments. Genotyping of diagnostic markers revealed the Rht-D1b mutant allele in 58% of the investigated varieties, while the Rht-B1b mutant was only present in 7% of the varieties. Rht-D1 was significantly associated with plant height by using a mixed linear model and employing a kinship matrix to correct for population stratification. Further genotyping data included 732 microsatellite markers, resulting in 770 loci, of which 635 markers were placed on the ITMI map plus a set of 7769 mapped SNP markers genotyped with the 90 k iSELECT chip. When Bonferroni correction was applied, a total of 153 significant marker-trait associations (MTAs) were observed for plant height and the SSR markers (-log10 (P-value) ≥ 4.82) and 280 (-log10 (P-value) ≥ 5.89) for the SNPs. Linear regression between the most effective markers and the BLUEs for plant height indicated additive effects for the MTAs of different chromosomal regions. Analysis of syntenic regions in the rice genome revealed closely linked rice genes related to gibberellin acid (GA) metabolism and perception, i.e. GA20 and GA2 oxidases orthologous to wheat chromosomes 1A, 2A, 3A, 3B, 5B, 5D and 7B, ent-kaurenoic acid oxidase orthologous to wheat chromosome 7A, ent-kaurene synthase on wheat chromosome 2B, as well as GA-receptors like DELLA genes orthologous to wheat chromosomes 4B, 4D and 7A and genes of the GID family orthologous to chromosomes 2B and 5B. The data indicated that besides the widely used GA-insensitive dwarfing genes Rht-B1 and Rht-D1 there is a wide spectrum of loci available that could be used for modulating plant height in variety development. |
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| id | doaj.art-d10ce9e3a77940c299aeafb6b36fea4e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T11:51:46Z |
| publishDate | 2014-01-01 |
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| spelling | doaj.art-d10ce9e3a77940c299aeafb6b36fea4e2022-12-21T18:26:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11328710.1371/journal.pone.0113287Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.).Christine D ZankeJie LingJörg PlieskeSonja KollersErhard EbmeyerViktor KorzunOdile ArgillierGunther StieweMaike HinzeKerstin NeumannMartin W GanalMarion S RöderThe genetic architecture of plant height was investigated in a set of 358 recent European winter wheat varieties plus 14 spring wheat varieties based on field data in eight environments. Genotyping of diagnostic markers revealed the Rht-D1b mutant allele in 58% of the investigated varieties, while the Rht-B1b mutant was only present in 7% of the varieties. Rht-D1 was significantly associated with plant height by using a mixed linear model and employing a kinship matrix to correct for population stratification. Further genotyping data included 732 microsatellite markers, resulting in 770 loci, of which 635 markers were placed on the ITMI map plus a set of 7769 mapped SNP markers genotyped with the 90 k iSELECT chip. When Bonferroni correction was applied, a total of 153 significant marker-trait associations (MTAs) were observed for plant height and the SSR markers (-log10 (P-value) ≥ 4.82) and 280 (-log10 (P-value) ≥ 5.89) for the SNPs. Linear regression between the most effective markers and the BLUEs for plant height indicated additive effects for the MTAs of different chromosomal regions. Analysis of syntenic regions in the rice genome revealed closely linked rice genes related to gibberellin acid (GA) metabolism and perception, i.e. GA20 and GA2 oxidases orthologous to wheat chromosomes 1A, 2A, 3A, 3B, 5B, 5D and 7B, ent-kaurenoic acid oxidase orthologous to wheat chromosome 7A, ent-kaurene synthase on wheat chromosome 2B, as well as GA-receptors like DELLA genes orthologous to wheat chromosomes 4B, 4D and 7A and genes of the GID family orthologous to chromosomes 2B and 5B. The data indicated that besides the widely used GA-insensitive dwarfing genes Rht-B1 and Rht-D1 there is a wide spectrum of loci available that could be used for modulating plant height in variety development.https://doi.org/10.1371/journal.pone.0113287 |
| spellingShingle | Christine D Zanke Jie Ling Jörg Plieske Sonja Kollers Erhard Ebmeyer Viktor Korzun Odile Argillier Gunther Stiewe Maike Hinze Kerstin Neumann Martin W Ganal Marion S Röder Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.). PLoS ONE |
| title | Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.). |
| title_full | Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.). |
| title_fullStr | Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.). |
| title_full_unstemmed | Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.). |
| title_short | Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.). |
| title_sort | whole genome association mapping of plant height in winter wheat triticum aestivum l |
| url | https://doi.org/10.1371/journal.pone.0113287 |
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