A dysflagellar mutant of <it>Leishmania (Viannia) braziliensis </it>isolated from a cutaneous leishmaniasis patient
<p>Abstract</p> <p>Background</p> <p>Parasites of the <it>Leishmania </it>genus alternate between the flagellated extracellular promastigote stage and intracellular amastigotes. Here we report the characterization of a <it>Leishmania </it>isolate...
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BMC
2012-01-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://www.parasitesandvectors.com/content/5/1/11 |
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| author | Zauli Rogéria C Yokoyama-Yasunaka Jenicer KU Miguel Danilo C Moura Alexandre S Pereira Ledice IA da Silva Ildefonso A Lemes Lucianna GN Dorta Miriam L de Oliveira Milton AP Pitaluga André N Ishikawa Edna AY Rodrigues Juliany CF Traub-Cseko Yara M Bijovsky A Ribeiro-Dias Fátima Uliana Silvia RB |
| author_facet | Zauli Rogéria C Yokoyama-Yasunaka Jenicer KU Miguel Danilo C Moura Alexandre S Pereira Ledice IA da Silva Ildefonso A Lemes Lucianna GN Dorta Miriam L de Oliveira Milton AP Pitaluga André N Ishikawa Edna AY Rodrigues Juliany CF Traub-Cseko Yara M Bijovsky A Ribeiro-Dias Fátima Uliana Silvia RB |
| author_sort | Zauli Rogéria C |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Parasites of the <it>Leishmania </it>genus alternate between the flagellated extracellular promastigote stage and intracellular amastigotes. Here we report the characterization of a <it>Leishmania </it>isolate, obtained from a cutaneous leishmaniasis patient, which presents peculiar morphological features.</p> <p>Methods</p> <p>The parasite was cultured <it>in vitro </it>and characterized morphologically using optical and electron microscopy. Identification was performed based on monoclonal antibodies and internal ribosomal spacer typing. <it>In vitro </it>macrophage cultures, murine experimental models and sand fly infections were used to evaluate infectivity <it>in vitro </it>and <it>in vivo</it>.</p> <p>Results</p> <p>The isolate was identified as <it>Leishmania </it>(<it>Viannia</it>) <it>braziliensis</it>. In the atypical promastigotes grown in culture, a short flagellum surrounded or interrupted by a protuberance of disorganized material was observed. A normal axoneme was present close to the basal body but without elongation much further outside the flagellar pocket. A disorganized swelling at the precocious end of the axoneme coincided with the lack of a paraflagellar rod structure. The isolate was able to infect macrophages <it>in vitro</it>, induce lesions in BALB/c mice and infect <it>Lutzomyia longipalpis</it>.</p> <p>Conclusions</p> <p>Notwithstanding the lack of an extracellular flagellum, this isolate infects macrophages <it>in vitro </it>and produces lesions when inoculated into mice. Moreover, it is able to colonize phlebotomine sand flies. Considering the importance attributed to the flagellum in the successful infection and survival of <it>Leishmania </it>in the insect midgut and in the invasion of macrophages, these findings may bring new light into the infectious mechanisms of <it>L</it>. (<it>V</it>.) <it>braziliensis</it>.</p> |
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| institution | Directory Open Access Journal |
| issn | 1756-3305 |
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| publishDate | 2012-01-01 |
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| spelling | doaj.art-d10e874c4f2e42559e7a40ea107504772022-12-21T21:43:18ZengBMCParasites & Vectors1756-33052012-01-01511110.1186/1756-3305-5-11A dysflagellar mutant of <it>Leishmania (Viannia) braziliensis </it>isolated from a cutaneous leishmaniasis patientZauli Rogéria CYokoyama-Yasunaka Jenicer KUMiguel Danilo CMoura Alexandre SPereira Ledice IAda Silva Ildefonso ALemes Lucianna GNDorta Miriam Lde Oliveira Milton APPitaluga André NIshikawa Edna AYRodrigues Juliany CFTraub-Cseko Yara MBijovsky ARibeiro-Dias FátimaUliana Silvia RB<p>Abstract</p> <p>Background</p> <p>Parasites of the <it>Leishmania </it>genus alternate between the flagellated extracellular promastigote stage and intracellular amastigotes. Here we report the characterization of a <it>Leishmania </it>isolate, obtained from a cutaneous leishmaniasis patient, which presents peculiar morphological features.</p> <p>Methods</p> <p>The parasite was cultured <it>in vitro </it>and characterized morphologically using optical and electron microscopy. Identification was performed based on monoclonal antibodies and internal ribosomal spacer typing. <it>In vitro </it>macrophage cultures, murine experimental models and sand fly infections were used to evaluate infectivity <it>in vitro </it>and <it>in vivo</it>.</p> <p>Results</p> <p>The isolate was identified as <it>Leishmania </it>(<it>Viannia</it>) <it>braziliensis</it>. In the atypical promastigotes grown in culture, a short flagellum surrounded or interrupted by a protuberance of disorganized material was observed. A normal axoneme was present close to the basal body but without elongation much further outside the flagellar pocket. A disorganized swelling at the precocious end of the axoneme coincided with the lack of a paraflagellar rod structure. The isolate was able to infect macrophages <it>in vitro</it>, induce lesions in BALB/c mice and infect <it>Lutzomyia longipalpis</it>.</p> <p>Conclusions</p> <p>Notwithstanding the lack of an extracellular flagellum, this isolate infects macrophages <it>in vitro </it>and produces lesions when inoculated into mice. Moreover, it is able to colonize phlebotomine sand flies. Considering the importance attributed to the flagellum in the successful infection and survival of <it>Leishmania </it>in the insect midgut and in the invasion of macrophages, these findings may bring new light into the infectious mechanisms of <it>L</it>. (<it>V</it>.) <it>braziliensis</it>.</p>http://www.parasitesandvectors.com/content/5/1/11flagellummutant<it>Leishmania</it>electron microscopy. |
| spellingShingle | Zauli Rogéria C Yokoyama-Yasunaka Jenicer KU Miguel Danilo C Moura Alexandre S Pereira Ledice IA da Silva Ildefonso A Lemes Lucianna GN Dorta Miriam L de Oliveira Milton AP Pitaluga André N Ishikawa Edna AY Rodrigues Juliany CF Traub-Cseko Yara M Bijovsky A Ribeiro-Dias Fátima Uliana Silvia RB A dysflagellar mutant of <it>Leishmania (Viannia) braziliensis </it>isolated from a cutaneous leishmaniasis patient Parasites & Vectors flagellum mutant <it>Leishmania</it> electron microscopy. |
| title | A dysflagellar mutant of <it>Leishmania (Viannia) braziliensis </it>isolated from a cutaneous leishmaniasis patient |
| title_full | A dysflagellar mutant of <it>Leishmania (Viannia) braziliensis </it>isolated from a cutaneous leishmaniasis patient |
| title_fullStr | A dysflagellar mutant of <it>Leishmania (Viannia) braziliensis </it>isolated from a cutaneous leishmaniasis patient |
| title_full_unstemmed | A dysflagellar mutant of <it>Leishmania (Viannia) braziliensis </it>isolated from a cutaneous leishmaniasis patient |
| title_short | A dysflagellar mutant of <it>Leishmania (Viannia) braziliensis </it>isolated from a cutaneous leishmaniasis patient |
| title_sort | dysflagellar mutant of it leishmania viannia braziliensis it isolated from a cutaneous leishmaniasis patient |
| topic | flagellum mutant <it>Leishmania</it> electron microscopy. |
| url | http://www.parasitesandvectors.com/content/5/1/11 |
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