THE ROLE OF PLASMIDS IN RESISTANCE OF Kl. pneumoniae ANTIBIOTICS
The bacterium Kl. pneumoniae was isolated from urine sample of patient with urinary tract infection and diagnosed according to its cultural and microscopic characters and biochemical reactions . Sensitivity test of the bacterium against antibiotics was established and the results appeared that the b...
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| Format: | Article |
| Language: | English |
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University of Anbar
2012-06-01
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| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_15444_ac5c3819546ecb980f8574891cb2ef79.pdf |
| _version_ | 1827396400251404288 |
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| author | KHALAF JASIM MOHAMMED AL-DOLAIMI |
| author_facet | KHALAF JASIM MOHAMMED AL-DOLAIMI |
| author_sort | KHALAF JASIM MOHAMMED AL-DOLAIMI |
| collection | DOAJ |
| description | The bacterium Kl. pneumoniae was isolated from urine sample of patient with urinary tract infection and diagnosed according to its cultural and microscopic characters and biochemical reactions . Sensitivity test of the bacterium against antibiotics was established and the results appeared that the bacterium was Sensitive to Amoxycillin , Gentamycin , Cephaloxin, Neomycin, Rifampicin , Chloramphenicol , Tobramycin , Ampicillin and carpenicillin and resistant to clindomycin ,cloxacillin , Trimethprim , Cefalotine , Vancomycin , Penicillin G , Lincomycin , Fusidic acid , Metronidazole and Nalidixic acid . Results of plasmid DNA content for the bacterium showed the presence of 8-6 kb plasmid compared with the plasmid pBR 322 isolated from E. coli HBlol . To study the role of plasmids in coding for antibiotics resistance , curing experiment was accomplished using SDS .Results showed the loss of resistance of the antibiotic , Nalidixic acid after physical loss of plasmid as ensured by gel electrophoresis. |
| first_indexed | 2024-03-08T18:50:24Z |
| format | Article |
| id | doaj.art-d110994df13a49e09e5a31c2495eba9b |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:50:24Z |
| publishDate | 2012-06-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-d110994df13a49e09e5a31c2495eba9b2023-12-28T21:51:42ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032012-06-0111465310.37652/juaps.2007.1544415444THE ROLE OF PLASMIDS IN RESISTANCE OF Kl. pneumoniae ANTIBIOTICSKHALAF JASIM MOHAMMED AL-DOLAIMI0Anbar University - College of Science - Department of Life SciencesThe bacterium Kl. pneumoniae was isolated from urine sample of patient with urinary tract infection and diagnosed according to its cultural and microscopic characters and biochemical reactions . Sensitivity test of the bacterium against antibiotics was established and the results appeared that the bacterium was Sensitive to Amoxycillin , Gentamycin , Cephaloxin, Neomycin, Rifampicin , Chloramphenicol , Tobramycin , Ampicillin and carpenicillin and resistant to clindomycin ,cloxacillin , Trimethprim , Cefalotine , Vancomycin , Penicillin G , Lincomycin , Fusidic acid , Metronidazole and Nalidixic acid . Results of plasmid DNA content for the bacterium showed the presence of 8-6 kb plasmid compared with the plasmid pBR 322 isolated from E. coli HBlol . To study the role of plasmids in coding for antibiotics resistance , curing experiment was accomplished using SDS .Results showed the loss of resistance of the antibiotic , Nalidixic acid after physical loss of plasmid as ensured by gel electrophoresis.https://juaps.uoanbar.edu.iq/article_15444_ac5c3819546ecb980f8574891cb2ef79.pdfpneumoniae |
| spellingShingle | KHALAF JASIM MOHAMMED AL-DOLAIMI THE ROLE OF PLASMIDS IN RESISTANCE OF Kl. pneumoniae ANTIBIOTICS مجلة جامعة الانبار للعلوم الصرفة pneumoniae |
| title | THE ROLE OF PLASMIDS IN RESISTANCE OF Kl. pneumoniae ANTIBIOTICS |
| title_full | THE ROLE OF PLASMIDS IN RESISTANCE OF Kl. pneumoniae ANTIBIOTICS |
| title_fullStr | THE ROLE OF PLASMIDS IN RESISTANCE OF Kl. pneumoniae ANTIBIOTICS |
| title_full_unstemmed | THE ROLE OF PLASMIDS IN RESISTANCE OF Kl. pneumoniae ANTIBIOTICS |
| title_short | THE ROLE OF PLASMIDS IN RESISTANCE OF Kl. pneumoniae ANTIBIOTICS |
| title_sort | role of plasmids in resistance of kl pneumoniae antibiotics |
| topic | pneumoniae |
| url | https://juaps.uoanbar.edu.iq/article_15444_ac5c3819546ecb980f8574891cb2ef79.pdf |
| work_keys_str_mv | AT khalafjasimmohammedaldolaimi theroleofplasmidsinresistanceofklpneumoniaeantibiotics AT khalafjasimmohammedaldolaimi roleofplasmidsinresistanceofklpneumoniaeantibiotics |