Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas
The current study investigated the capacity for tumor factors secreted by head and neck squamous cell carcinoma (HNSCC) cell lines, KB, KB16, and HEP, to induce the secretion of various cytokines from peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from blood samples collected from s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2011-10-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000500007 |
| _version_ | 1818583261104832512 |
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| author | Cristiane Miranda França Fabiana Mesquita Barros Monica Andrade Lotufo Kristianne Porta Santos Fernandes Ricardo Carneiro Borra |
| author_facet | Cristiane Miranda França Fabiana Mesquita Barros Monica Andrade Lotufo Kristianne Porta Santos Fernandes Ricardo Carneiro Borra |
| author_sort | Cristiane Miranda França |
| collection | DOAJ |
| description | The current study investigated the capacity for tumor factors secreted by head and neck squamous cell carcinoma (HNSCC) cell lines, KB, KB16, and HEP, to induce the secretion of various cytokines from peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from blood samples collected from six healthy volunteers and these cells were incubated for 6, 24, 48, or 72 hours in the presence of 50% conditioned medium collected from cultured cell lines pretreated with, or without, stimulants such as phytohemagglutinin (PHA) or lipopolysaccharides (LPS). Aliquots of each supernatant were then assayed for levels of IFN-Γ, vascular endothelial growth factor (VEGF), TNF-α, and IL-4 using enzyme linked immunosorbent assays (ELISAs). Data collected were analyzed using Student's t-test, an ANOVA test followed by Tukey's test, and tests of Pearson's Correlation. PBMCs cultured with KB16-conditioned medium produced the highest levels of IFN-Γ. VEGF was also detected in conditioned media collected from all of the squamous cell carcinoma (SCC) cell lines used, and a significant difference in VEGF levels between control and KB- or KB16-conditioned media was observed. TNF-α was secreted by all PBMC groups within 6 hours of receiving conditioned media, and these levels increased up to the 24 hour timepoint, after which levels of TNF-α stabilized. In contrast, none of the supernatant samples contained detectable levels of IL-4. In combination, these data suggest that direct contact between fresh human PBMCs and conditioned media from tumor cells induces the secretion of TNF-α and VEGF by PBMCs, and this represents an initial angiogenic response. |
| first_indexed | 2024-12-16T08:02:28Z |
| format | Article |
| id | doaj.art-d114c5bfd9f443f497e3bf74fede25fc |
| institution | Directory Open Access Journal |
| issn | 1806-8324 |
| language | English |
| last_indexed | 2024-12-16T08:02:28Z |
| publishDate | 2011-10-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-d114c5bfd9f443f497e3bf74fede25fc2022-12-21T22:38:34ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1806-83242011-10-0125541442010.1590/S1806-83242011000500007Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomasCristiane Miranda FrançaFabiana Mesquita BarrosMonica Andrade LotufoKristianne Porta Santos FernandesRicardo Carneiro BorraThe current study investigated the capacity for tumor factors secreted by head and neck squamous cell carcinoma (HNSCC) cell lines, KB, KB16, and HEP, to induce the secretion of various cytokines from peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from blood samples collected from six healthy volunteers and these cells were incubated for 6, 24, 48, or 72 hours in the presence of 50% conditioned medium collected from cultured cell lines pretreated with, or without, stimulants such as phytohemagglutinin (PHA) or lipopolysaccharides (LPS). Aliquots of each supernatant were then assayed for levels of IFN-Γ, vascular endothelial growth factor (VEGF), TNF-α, and IL-4 using enzyme linked immunosorbent assays (ELISAs). Data collected were analyzed using Student's t-test, an ANOVA test followed by Tukey's test, and tests of Pearson's Correlation. PBMCs cultured with KB16-conditioned medium produced the highest levels of IFN-Γ. VEGF was also detected in conditioned media collected from all of the squamous cell carcinoma (SCC) cell lines used, and a significant difference in VEGF levels between control and KB- or KB16-conditioned media was observed. TNF-α was secreted by all PBMC groups within 6 hours of receiving conditioned media, and these levels increased up to the 24 hour timepoint, after which levels of TNF-α stabilized. In contrast, none of the supernatant samples contained detectable levels of IL-4. In combination, these data suggest that direct contact between fresh human PBMCs and conditioned media from tumor cells induces the secretion of TNF-α and VEGF by PBMCs, and this represents an initial angiogenic response.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000500007Mouth NeoplasmsCytokinesIn vitro |
| spellingShingle | Cristiane Miranda França Fabiana Mesquita Barros Monica Andrade Lotufo Kristianne Porta Santos Fernandes Ricardo Carneiro Borra Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas Brazilian Oral Research Mouth Neoplasms Cytokines In vitro |
| title | Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas |
| title_full | Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas |
| title_fullStr | Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas |
| title_full_unstemmed | Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas |
| title_short | Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas |
| title_sort | response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas |
| topic | Mouth Neoplasms Cytokines In vitro |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000500007 |
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