Simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography-tandem mass spectrometry method
Abstract Objective To establish a high-performance liquid chromatography–tandem mass spectrometry method (HPLC–MS/MS) to simultaneously determine colistin sulfate and tigecycline in human plasma. Methods Polymyxin B1 internal standard (20 µL) was added into 200 µL of plasma sample. The samples were...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Chemistry |
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| Online Access: | https://doi.org/10.1186/s13065-023-01109-8 |
| _version_ | 1797356020009271296 |
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| author | Ying-Chao Ma Xi-Kun Wu Xiu-Ling Yang Zhi-Qing Zhang |
| author_facet | Ying-Chao Ma Xi-Kun Wu Xiu-Ling Yang Zhi-Qing Zhang |
| author_sort | Ying-Chao Ma |
| collection | DOAJ |
| description | Abstract Objective To establish a high-performance liquid chromatography–tandem mass spectrometry method (HPLC–MS/MS) to simultaneously determine colistin sulfate and tigecycline in human plasma. Methods Polymyxin B1 internal standard (20 µL) was added into 200 µL of plasma sample. The samples were treated with methanol-5% trichloroacetic acid (50:50, V/V) solution, and the protein precipitation method was adopted for post-injection analysis. The chromatographic column was a Dikma C18 (4.6 mm × 150 mm, 5 μm). For the mobile phase, 0.1% formic acid in aqueous solution was used for phase A, 0.1% formic acid in acetonitrile solution for phase B, and gradient elution was also applied. The flow rate was 0.8 mL/min, the column temperature was 40 °C, and the injection volume was 10 µL; Electrospray ionization and multiple reaction ion monitoring were adopted and scanned by the HPLC–MS/MS positive ion mode. Results The endogenous impurities in the plasma had no interference in the determination of the analytes. There existed a good linear relationship of colistin sulfate within the range of 0.1–10 µg/mL (R2 = 0.9986), with the lower limit of quantification (LLOQ) of 0.1 µg/mL. There existed a good linear relationship of tigecycline within the range of 0.05–5 µg/ mL (R2 = 0.9987), with the LLOQ of 0.05 µg/mL. The intra- and inter-day relative standard deviations of colistin sulfate and tigecycline were both less than 15%, and the accuracy was between 88.21% and 108.24%. The extraction had good stability, the extraction recovery rate was 87.75–91.22%, and the matrix effect was 99.40–105.26%. Conclusion This study successfully established a method for simultaneously detecting colistin sulfate and tigecycline plasma concentrations. The method was simple, rapid, and highly sensitive and could be applied for therapeutic medication monitoring. |
| first_indexed | 2024-03-08T14:19:35Z |
| format | Article |
| id | doaj.art-d115f9c0f38e4c0fba16a5a921b93b81 |
| institution | Directory Open Access Journal |
| issn | 2661-801X |
| language | English |
| last_indexed | 2024-03-08T14:19:35Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Chemistry |
| spelling | doaj.art-d115f9c0f38e4c0fba16a5a921b93b812024-01-14T12:07:40ZengBMCBMC Chemistry2661-801X2024-01-0118111310.1186/s13065-023-01109-8Simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography-tandem mass spectrometry methodYing-Chao Ma0Xi-Kun Wu1Xiu-Ling Yang2Zhi-Qing Zhang3Department of Pharmacy, Second Hospital of Hebei Medical UniversityDepartment of Pharmacy, Second Hospital of Hebei Medical UniversityDepartment of Pharmacy, Second Hospital of Hebei Medical UniversityDepartment of Pharmacy, Second Hospital of Hebei Medical UniversityAbstract Objective To establish a high-performance liquid chromatography–tandem mass spectrometry method (HPLC–MS/MS) to simultaneously determine colistin sulfate and tigecycline in human plasma. Methods Polymyxin B1 internal standard (20 µL) was added into 200 µL of plasma sample. The samples were treated with methanol-5% trichloroacetic acid (50:50, V/V) solution, and the protein precipitation method was adopted for post-injection analysis. The chromatographic column was a Dikma C18 (4.6 mm × 150 mm, 5 μm). For the mobile phase, 0.1% formic acid in aqueous solution was used for phase A, 0.1% formic acid in acetonitrile solution for phase B, and gradient elution was also applied. The flow rate was 0.8 mL/min, the column temperature was 40 °C, and the injection volume was 10 µL; Electrospray ionization and multiple reaction ion monitoring were adopted and scanned by the HPLC–MS/MS positive ion mode. Results The endogenous impurities in the plasma had no interference in the determination of the analytes. There existed a good linear relationship of colistin sulfate within the range of 0.1–10 µg/mL (R2 = 0.9986), with the lower limit of quantification (LLOQ) of 0.1 µg/mL. There existed a good linear relationship of tigecycline within the range of 0.05–5 µg/ mL (R2 = 0.9987), with the LLOQ of 0.05 µg/mL. The intra- and inter-day relative standard deviations of colistin sulfate and tigecycline were both less than 15%, and the accuracy was between 88.21% and 108.24%. The extraction had good stability, the extraction recovery rate was 87.75–91.22%, and the matrix effect was 99.40–105.26%. Conclusion This study successfully established a method for simultaneously detecting colistin sulfate and tigecycline plasma concentrations. The method was simple, rapid, and highly sensitive and could be applied for therapeutic medication monitoring.https://doi.org/10.1186/s13065-023-01109-8Colistin sulfateTigecyclineLiquid chromatography–tandem mass spectrometryPlasma drug concentrationTherapeutic medication monitoring |
| spellingShingle | Ying-Chao Ma Xi-Kun Wu Xiu-Ling Yang Zhi-Qing Zhang Simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography-tandem mass spectrometry method BMC Chemistry Colistin sulfate Tigecycline Liquid chromatography–tandem mass spectrometry Plasma drug concentration Therapeutic medication monitoring |
| title | Simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography-tandem mass spectrometry method |
| title_full | Simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography-tandem mass spectrometry method |
| title_fullStr | Simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography-tandem mass spectrometry method |
| title_full_unstemmed | Simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography-tandem mass spectrometry method |
| title_short | Simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography-tandem mass spectrometry method |
| title_sort | simultaneous determination of colistin sulfate and tigecycline in human plasma by liquid chromatography tandem mass spectrometry method |
| topic | Colistin sulfate Tigecycline Liquid chromatography–tandem mass spectrometry Plasma drug concentration Therapeutic medication monitoring |
| url | https://doi.org/10.1186/s13065-023-01109-8 |
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