Alternative Feed Raw Materials Modulate Intestinal Microbiota and Its Relationship with Digestibility in Yellowtail Kingfish <i>Seriola lalandi</i>
Gut microbiota plays a crucial role in nutrient digestibility and fish health. This study aimed to investigate the effects of alternative feed raw materials on the bacterial communities in the distal intestine and its relationship with nutrient digestibility in yellowtail kingfish (YTK), <i>Se...
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MDPI AG
2020-05-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/5/2/14 |
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| author | Chinh Thi My Dam Mark Booth Igor Pirozzi Michael Salini Richard Smullen Tomer Ventura Abigail Elizur |
| author_facet | Chinh Thi My Dam Mark Booth Igor Pirozzi Michael Salini Richard Smullen Tomer Ventura Abigail Elizur |
| author_sort | Chinh Thi My Dam |
| collection | DOAJ |
| description | Gut microbiota plays a crucial role in nutrient digestibility and fish health. This study aimed to investigate the effects of alternative feed raw materials on the bacterial communities in the distal intestine and its relationship with nutrient digestibility in yellowtail kingfish (YTK), <i>Seriola lalandi</i>. Two 4-week digestibility trials were conducted to evaluate fish meal (FM), two sources of poultry by-product meal (PBM-1 & PBM-2), blood meal (BLM), faba bean meal (FBM), corn gluten meal (CGM), soy protein concentrate (SPC) and wheat flour (WH). The nutrient digestibility value was determined using the stripping fecal collection method. Bacterial communities were characterized by high-throughput sequencing based on V3-V4 region of the 16S rRNA gene. The most abundant phylum identified in the present study was <i>Proteobacteria.</i> A significant change in the distal intestine was observed in fish fed diets containing CGM and BLM, characterized by a reduction of species richness and diversity. Additionally, significant correlation between nutrient digestibility and intestinal microbiota was observed. <i>Allivibrio</i>, <i>Vibrio</i>, <i>Curvibacter</i>, <i>Ruminococcaceae</i>, and <i>Clostridium</i> were positively correlated, whereas <i>Ralstonia</i> genus was negatively correlated with nutrient digestibility. This study demonstrated that intestinal microbiota could be a useful tool for evaluating the digestibility of feed raw materials; however, further culture-based study is needed to confirm this observation. |
| first_indexed | 2024-03-10T19:54:52Z |
| format | Article |
| id | doaj.art-d116d859b2804232907d0e87ade870eb |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-10T19:54:52Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-d116d859b2804232907d0e87ade870eb2023-11-20T00:02:02ZengMDPI AGFishes2410-38882020-05-015214010.3390/fishes5020014Alternative Feed Raw Materials Modulate Intestinal Microbiota and Its Relationship with Digestibility in Yellowtail Kingfish <i>Seriola lalandi</i>Chinh Thi My Dam0Mark Booth1Igor Pirozzi2Michael Salini3Richard Smullen4Tomer Ventura5Abigail Elizur6Genecology Research Centre, School of Science and Engineering, University of the Sunshine Coast, Sippy Downs, QLD 4556, AustraliaNew South Wales Department of Primary Industries, Port Stephens Fisheries Institute, Taylors Beach, NSW 2316, AustraliaNew South Wales Department of Primary Industries, Port Stephens Fisheries Institute, Taylors Beach, NSW 2316, AustraliaRidley Aquafeed Pty Ltd., 4/31 Robart Court, Narangba, QLD 4504, AustraliaRidley Aquafeed Pty Ltd., 4/31 Robart Court, Narangba, QLD 4504, AustraliaGenecology Research Centre, School of Science and Engineering, University of the Sunshine Coast, Sippy Downs, QLD 4556, AustraliaGenecology Research Centre, School of Science and Engineering, University of the Sunshine Coast, Sippy Downs, QLD 4556, AustraliaGut microbiota plays a crucial role in nutrient digestibility and fish health. This study aimed to investigate the effects of alternative feed raw materials on the bacterial communities in the distal intestine and its relationship with nutrient digestibility in yellowtail kingfish (YTK), <i>Seriola lalandi</i>. Two 4-week digestibility trials were conducted to evaluate fish meal (FM), two sources of poultry by-product meal (PBM-1 & PBM-2), blood meal (BLM), faba bean meal (FBM), corn gluten meal (CGM), soy protein concentrate (SPC) and wheat flour (WH). The nutrient digestibility value was determined using the stripping fecal collection method. Bacterial communities were characterized by high-throughput sequencing based on V3-V4 region of the 16S rRNA gene. The most abundant phylum identified in the present study was <i>Proteobacteria.</i> A significant change in the distal intestine was observed in fish fed diets containing CGM and BLM, characterized by a reduction of species richness and diversity. Additionally, significant correlation between nutrient digestibility and intestinal microbiota was observed. <i>Allivibrio</i>, <i>Vibrio</i>, <i>Curvibacter</i>, <i>Ruminococcaceae</i>, and <i>Clostridium</i> were positively correlated, whereas <i>Ralstonia</i> genus was negatively correlated with nutrient digestibility. This study demonstrated that intestinal microbiota could be a useful tool for evaluating the digestibility of feed raw materials; however, further culture-based study is needed to confirm this observation.https://www.mdpi.com/2410-3888/5/2/14Yellowtail kingfishalternative raw feed materialsdistal intestinal microbiota16S rRNA gene amplicon sequencingdigestibilityfish nutrition |
| spellingShingle | Chinh Thi My Dam Mark Booth Igor Pirozzi Michael Salini Richard Smullen Tomer Ventura Abigail Elizur Alternative Feed Raw Materials Modulate Intestinal Microbiota and Its Relationship with Digestibility in Yellowtail Kingfish <i>Seriola lalandi</i> Fishes Yellowtail kingfish alternative raw feed materials distal intestinal microbiota 16S rRNA gene amplicon sequencing digestibility fish nutrition |
| title | Alternative Feed Raw Materials Modulate Intestinal Microbiota and Its Relationship with Digestibility in Yellowtail Kingfish <i>Seriola lalandi</i> |
| title_full | Alternative Feed Raw Materials Modulate Intestinal Microbiota and Its Relationship with Digestibility in Yellowtail Kingfish <i>Seriola lalandi</i> |
| title_fullStr | Alternative Feed Raw Materials Modulate Intestinal Microbiota and Its Relationship with Digestibility in Yellowtail Kingfish <i>Seriola lalandi</i> |
| title_full_unstemmed | Alternative Feed Raw Materials Modulate Intestinal Microbiota and Its Relationship with Digestibility in Yellowtail Kingfish <i>Seriola lalandi</i> |
| title_short | Alternative Feed Raw Materials Modulate Intestinal Microbiota and Its Relationship with Digestibility in Yellowtail Kingfish <i>Seriola lalandi</i> |
| title_sort | alternative feed raw materials modulate intestinal microbiota and its relationship with digestibility in yellowtail kingfish i seriola lalandi i |
| topic | Yellowtail kingfish alternative raw feed materials distal intestinal microbiota 16S rRNA gene amplicon sequencing digestibility fish nutrition |
| url | https://www.mdpi.com/2410-3888/5/2/14 |
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