Isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty)
The heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) (HAHp-H) were fractioned using Bio-Gel P-4 and Sephadex G 25 chromatography, and RP-HPLC to obtain peptides with antiproliferative activities on human prostate cancer PC-3 cells. One peptide was identified with the seque...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-09-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614002011 |
| _version_ | 1818958113399635968 |
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| author | Ru Song Rong-bian Wei Hong-yu Luo Zui-su Yang |
| author_facet | Ru Song Rong-bian Wei Hong-yu Luo Zui-su Yang |
| author_sort | Ru Song |
| collection | DOAJ |
| description | The heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) (HAHp-H) were fractioned using Bio-Gel P-4 and Sephadex G 25 chromatography, and RP-HPLC to obtain peptides with antiproliferative activities on human prostate cancer PC-3 cells. One peptide was identified with the sequence of YALPAH. The synthetic YALPAH induced PC-3 cell apoptosis at the concentration of 4.47 μM. Three analogous peptides, i.e., YALRAH, YALPAR and YALPAG, were also synthesized. All these synthetic peptides displayed concentration-dependent manners in inhibiting the growth of PC-3 cells. Furthermore, peptide YALRAH with the highest positive charge exhibited the strongest antiproliferation, with IC50 value of 8.1 mg/ml (or 11.1 μM). While as with the same net charges, the lower molecular weight of YALPAH (670.77 Da) was proposed to be responsible for its higher cytotoxicity compared with YALPAR (689.81 Da). This is the first report of an antiproliferative peptide on PC-3 cells derived from half-fin anchovy. |
| first_indexed | 2024-12-20T11:20:35Z |
| format | Article |
| id | doaj.art-d1194fdbb32a42c583b6d57b6df4ac0e |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T11:20:35Z |
| publishDate | 2014-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-d1194fdbb32a42c583b6d57b6df4ac0e2022-12-21T19:42:31ZengElsevierJournal of Functional Foods1756-46462014-09-0110104111Isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty)Ru Song0Rong-bian Wei1Hong-yu Luo2Zui-su Yang3College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China; Corresponding author. Tel.: +86 580 2554781; fax: +86 580 2554781.College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaCollege of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaCollege of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaThe heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) (HAHp-H) were fractioned using Bio-Gel P-4 and Sephadex G 25 chromatography, and RP-HPLC to obtain peptides with antiproliferative activities on human prostate cancer PC-3 cells. One peptide was identified with the sequence of YALPAH. The synthetic YALPAH induced PC-3 cell apoptosis at the concentration of 4.47 μM. Three analogous peptides, i.e., YALRAH, YALPAR and YALPAG, were also synthesized. All these synthetic peptides displayed concentration-dependent manners in inhibiting the growth of PC-3 cells. Furthermore, peptide YALRAH with the highest positive charge exhibited the strongest antiproliferation, with IC50 value of 8.1 mg/ml (or 11.1 μM). While as with the same net charges, the lower molecular weight of YALPAH (670.77 Da) was proposed to be responsible for its higher cytotoxicity compared with YALPAR (689.81 Da). This is the first report of an antiproliferative peptide on PC-3 cells derived from half-fin anchovy.http://www.sciencedirect.com/science/article/pii/S1756464614002011Half-fin anchovyHeated peptic hydrolysatesAntiproliferative peptideHuman prostate cancer PC-3 cellAmino acid sequence |
| spellingShingle | Ru Song Rong-bian Wei Hong-yu Luo Zui-su Yang Isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) Journal of Functional Foods Half-fin anchovy Heated peptic hydrolysates Antiproliferative peptide Human prostate cancer PC-3 cell Amino acid sequence |
| title | Isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) |
| title_full | Isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) |
| title_fullStr | Isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) |
| title_full_unstemmed | Isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) |
| title_short | Isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half-fin anchovy (Setipinna taty) |
| title_sort | isolation and identification of an antiproliferative peptide derived from heated products of peptic hydrolysates of half fin anchovy setipinna taty |
| topic | Half-fin anchovy Heated peptic hydrolysates Antiproliferative peptide Human prostate cancer PC-3 cell Amino acid sequence |
| url | http://www.sciencedirect.com/science/article/pii/S1756464614002011 |
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