Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander

The extraction process of crude glycoprotein from Chinese giant salamander (MGP) mucus cultured in Lishui was optimized. The crude glycoprotein was further purified, of which characterization was analysed. Based on single factor experiment, the extraction process was optimized by response surface me...

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Main Authors: Jiacheng TANG, Jianqiang BAO, Yanjie CHEN, Qianyi SHAN, Kecheng HUANG, Xuan GONG
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2022-11-01
Series:Shipin gongye ke-ji
Subjects:
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030284
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author Jiacheng TANG
Jianqiang BAO
Yanjie CHEN
Qianyi SHAN
Kecheng HUANG
Xuan GONG
author_facet Jiacheng TANG
Jianqiang BAO
Yanjie CHEN
Qianyi SHAN
Kecheng HUANG
Xuan GONG
author_sort Jiacheng TANG
collection DOAJ
description The extraction process of crude glycoprotein from Chinese giant salamander (MGP) mucus cultured in Lishui was optimized. The crude glycoprotein was further purified, of which characterization was analysed. Based on single factor experiment, the extraction process was optimized by response surface methodology with solid-liquid ratio, temperature, extraction concentration, extraction time as factors, and glycoprotein yield as index. The results showed that the optimal extraction conditions were solid-liquid ratio of 1:30.5 g/mL, extraction temperature of 51 ℃, extraction concentration of 0.32 mol/L, extraction time of 5.2 h, the highest yield of MGP was 5.22%±0.71%, which was basically consistent with the expected results. The electrophoresis pure of purified glycoprotein was determined. The lyophilized MGP was white, loose powder, of which molecular weight was about 29 kDa. The contents of essential amino acids and medicinal amino acids in MGP were 37.21 g/100 g and 33.39 g/100 g, respectively. MGP had a typical glycoprotein structure of UV full wavelength absorption image, containing o-glycopeptide bond, pyranose ring structure and β-glycosidic bond. The secondary structure of MGP was mainly composed of 87% β-folded, which provided a theoretical basis for further research on the biological activity of glycoprotein from the mucus of Chinese giant salamander.
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spelling doaj.art-d11ed04eb7ed4cd297cfafd3eeb954652022-12-22T04:14:52ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-11-01432125226110.13386/j.issn1002-0306.20220302842022030284-21Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant SalamanderJiacheng TANG0Jianqiang BAO1Yanjie CHEN2Qianyi SHAN3Kecheng HUANG4Xuan GONG5College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaThe extraction process of crude glycoprotein from Chinese giant salamander (MGP) mucus cultured in Lishui was optimized. The crude glycoprotein was further purified, of which characterization was analysed. Based on single factor experiment, the extraction process was optimized by response surface methodology with solid-liquid ratio, temperature, extraction concentration, extraction time as factors, and glycoprotein yield as index. The results showed that the optimal extraction conditions were solid-liquid ratio of 1:30.5 g/mL, extraction temperature of 51 ℃, extraction concentration of 0.32 mol/L, extraction time of 5.2 h, the highest yield of MGP was 5.22%±0.71%, which was basically consistent with the expected results. The electrophoresis pure of purified glycoprotein was determined. The lyophilized MGP was white, loose powder, of which molecular weight was about 29 kDa. The contents of essential amino acids and medicinal amino acids in MGP were 37.21 g/100 g and 33.39 g/100 g, respectively. MGP had a typical glycoprotein structure of UV full wavelength absorption image, containing o-glycopeptide bond, pyranose ring structure and β-glycosidic bond. The secondary structure of MGP was mainly composed of 87% β-folded, which provided a theoretical basis for further research on the biological activity of glycoprotein from the mucus of Chinese giant salamander.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030284chinese giant salamander mucus glycoprotein (mgp)extraction processyieldamino acid compositioncharacterization analysis
spellingShingle Jiacheng TANG
Jianqiang BAO
Yanjie CHEN
Qianyi SHAN
Kecheng HUANG
Xuan GONG
Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander
Shipin gongye ke-ji
chinese giant salamander mucus glycoprotein (mgp)
extraction process
yield
amino acid composition
characterization analysis
title Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander
title_full Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander
title_fullStr Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander
title_full_unstemmed Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander
title_short Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander
title_sort extraction process optimization purification and characterization analysis of glycoprotein from the mucus of chinese giant salamander
topic chinese giant salamander mucus glycoprotein (mgp)
extraction process
yield
amino acid composition
characterization analysis
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030284
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