Host Genes Involved in Nodulation Preference in Common Bean (Phaseolus vulgaris)–Rhizobium etli Symbiosis Revealed by Suppressive Subtractive Hybridization
Common bean cultivars are nodulated preferentially by Rhizobium etli lineages from the same center of host diversification. Nodulation was found to be earlier and numerous in bean plants inoculated with the cognate strain. We predicted that analysis of transcripts at early stages of the interaction...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2008-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-4-0459 |
| _version_ | 1811291290253393920 |
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| author | Eitel Peltzer Meschini Flavio Antonio Blanco María Eugenia Zanetti María Pía Beker Helge Küster Alfred Pühler O. Mario Aguilar |
| author_facet | Eitel Peltzer Meschini Flavio Antonio Blanco María Eugenia Zanetti María Pía Beker Helge Küster Alfred Pühler O. Mario Aguilar |
| author_sort | Eitel Peltzer Meschini |
| collection | DOAJ |
| description | Common bean cultivars are nodulated preferentially by Rhizobium etli lineages from the same center of host diversification. Nodulation was found to be earlier and numerous in bean plants inoculated with the cognate strain. We predicted that analysis of transcripts at early stages of the interaction between host and rhizobium would identify plant genes that are most likely to be involved in this preferential nodulation. Therefore, we applied a suppressive subtractive hybridization approach in which cDNA from a Mesoamerican cultivar inoculated with either the more- or less-efficient strain of R. etli was used as the driver and the tester, respectively. Forty-one independent tentative consensus sequences (TCs) were obtained and classified into different functional categories. Of 11 selected TCs, 9 were confirmed by quantitative reverse-transcriptase polymerase chain reaction. Two genes show high homology to previously characterized plant receptors. Two other upregulated genes encode for Rab11, a member of the small GTP-binding protein family, and HAP5, a subunit of the heterotrimeric CCAAT-transcription factor. Interestingly, one of the TCs encodes for an isoflavone reductase, which may lead to earlier Nod factor production by specific strains of rhizobia. The transcript abundance of selected cDNAs also was found to be higher in mature nodules of the more efficient interaction. Small or no differences were observed when an Andean bean cultivar was inoculated with a cognate strain, suggesting involvement of these genes in the strain-specific response. The potential role of these genes in the early preferential symbiotic interaction is discussed. |
| first_indexed | 2024-04-13T04:26:12Z |
| format | Article |
| id | doaj.art-d121a5d266fe4fef83ccfbb70f2fec98 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:26:12Z |
| publishDate | 2008-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-d121a5d266fe4fef83ccfbb70f2fec982022-12-22T03:02:30ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062008-04-0121445946810.1094/MPMI-21-4-0459Host Genes Involved in Nodulation Preference in Common Bean (Phaseolus vulgaris)–Rhizobium etli Symbiosis Revealed by Suppressive Subtractive HybridizationEitel Peltzer MeschiniFlavio Antonio BlancoMaría Eugenia ZanettiMaría Pía BekerHelge KüsterAlfred PühlerO. Mario AguilarCommon bean cultivars are nodulated preferentially by Rhizobium etli lineages from the same center of host diversification. Nodulation was found to be earlier and numerous in bean plants inoculated with the cognate strain. We predicted that analysis of transcripts at early stages of the interaction between host and rhizobium would identify plant genes that are most likely to be involved in this preferential nodulation. Therefore, we applied a suppressive subtractive hybridization approach in which cDNA from a Mesoamerican cultivar inoculated with either the more- or less-efficient strain of R. etli was used as the driver and the tester, respectively. Forty-one independent tentative consensus sequences (TCs) were obtained and classified into different functional categories. Of 11 selected TCs, 9 were confirmed by quantitative reverse-transcriptase polymerase chain reaction. Two genes show high homology to previously characterized plant receptors. Two other upregulated genes encode for Rab11, a member of the small GTP-binding protein family, and HAP5, a subunit of the heterotrimeric CCAAT-transcription factor. Interestingly, one of the TCs encodes for an isoflavone reductase, which may lead to earlier Nod factor production by specific strains of rhizobia. The transcript abundance of selected cDNAs also was found to be higher in mature nodules of the more efficient interaction. Small or no differences were observed when an Andean bean cultivar was inoculated with a cognate strain, suggesting involvement of these genes in the strain-specific response. The potential role of these genes in the early preferential symbiotic interaction is discussed.https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-4-0459legumeroot hair |
| spellingShingle | Eitel Peltzer Meschini Flavio Antonio Blanco María Eugenia Zanetti María Pía Beker Helge Küster Alfred Pühler O. Mario Aguilar Host Genes Involved in Nodulation Preference in Common Bean (Phaseolus vulgaris)–Rhizobium etli Symbiosis Revealed by Suppressive Subtractive Hybridization Molecular Plant-Microbe Interactions legume root hair |
| title | Host Genes Involved in Nodulation Preference in Common Bean (Phaseolus vulgaris)–Rhizobium etli Symbiosis Revealed by Suppressive Subtractive Hybridization |
| title_full | Host Genes Involved in Nodulation Preference in Common Bean (Phaseolus vulgaris)–Rhizobium etli Symbiosis Revealed by Suppressive Subtractive Hybridization |
| title_fullStr | Host Genes Involved in Nodulation Preference in Common Bean (Phaseolus vulgaris)–Rhizobium etli Symbiosis Revealed by Suppressive Subtractive Hybridization |
| title_full_unstemmed | Host Genes Involved in Nodulation Preference in Common Bean (Phaseolus vulgaris)–Rhizobium etli Symbiosis Revealed by Suppressive Subtractive Hybridization |
| title_short | Host Genes Involved in Nodulation Preference in Common Bean (Phaseolus vulgaris)–Rhizobium etli Symbiosis Revealed by Suppressive Subtractive Hybridization |
| title_sort | host genes involved in nodulation preference in common bean phaseolus vulgaris rhizobium etli symbiosis revealed by suppressive subtractive hybridization |
| topic | legume root hair |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-4-0459 |
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