Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression
Introduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation. Materials and Methods: A cytot...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2018;volume=10;issue=1;spage=43;epage=47;aulast=Anwar |
| _version_ | 1828167223488806912 |
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| author | Iwan B Anwar Asep Santoso Eko Saputra Rifky Ismail J Jamari Emile van der Heide |
| author_facet | Iwan B Anwar Asep Santoso Eko Saputra Rifky Ismail J Jamari Emile van der Heide |
| author_sort | Iwan B Anwar |
| collection | DOAJ |
| description | Introduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation. Materials and Methods: A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay. Cell viability was measured with Inhibitory concentration 50% (IC50) formula. Results: All treatment groups showed a cell viability of >50% ranging from 78% to >100%. Lower expression of IL-6 of hBMSC compared to control group was found in 48h of incubation period. Conclusion: hBMSC showed high cell viability after initial contact with UHMWPE material. Modulation of IL-6 expression was present at the initial stage as a response to foreign material. |
| first_indexed | 2024-04-12T02:15:02Z |
| format | Article |
| id | doaj.art-d1225bedae6540ddbf4f81dad02f5c80 |
| institution | Directory Open Access Journal |
| issn | 0975-7406 |
| language | English |
| last_indexed | 2024-04-12T02:15:02Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj.art-d1225bedae6540ddbf4f81dad02f5c802022-12-22T03:52:17ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0975-74062018-01-01101434710.4103/jpbs.JPBS_70_17Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expressionIwan B AnwarAsep SantosoEko SaputraRifky IsmailJ JamariEmile van der HeideIntroduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation. Materials and Methods: A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay. Cell viability was measured with Inhibitory concentration 50% (IC50) formula. Results: All treatment groups showed a cell viability of >50% ranging from 78% to >100%. Lower expression of IL-6 of hBMSC compared to control group was found in 48h of incubation period. Conclusion: hBMSC showed high cell viability after initial contact with UHMWPE material. Modulation of IL-6 expression was present at the initial stage as a response to foreign material.http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2018;volume=10;issue=1;spage=43;epage=47;aulast=AnwarCell viabilityhuman stem cellsinterleukin-6UHMWPE |
| spellingShingle | Iwan B Anwar Asep Santoso Eko Saputra Rifky Ismail J Jamari Emile van der Heide Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression Journal of Pharmacy and Bioallied Sciences Cell viability human stem cells interleukin-6 UHMWPE |
| title | Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression |
| title_full | Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression |
| title_fullStr | Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression |
| title_full_unstemmed | Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression |
| title_short | Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression |
| title_sort | initial response of human bone marrow derived stem cells after contact with ultrahigh molecular weight polyethylene uhmwpe material an in vitro study on cell viability and interleukin 6 expression |
| topic | Cell viability human stem cells interleukin-6 UHMWPE |
| url | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2018;volume=10;issue=1;spage=43;epage=47;aulast=Anwar |
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