Molecular Characteristics and Antimicrobial Susceptibility Profiles of blaKPC-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, China
Shuaijun Cao,1,* Xiaoying Jiang,2,* Jinshan Suo,3 Yanyan Lu,2 Mohan Ju,2 Qixiang Zeng,1 Qingru Zheng,1 Zuoyan Zhang,1 Wenqi Tang1 1Department of Critical Care Medicine, Shanghai Sixth People’s Hospital, Shanghai, People’s Republic of China; 2Institute of Antibiotics, Huashan Hospital...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2024-01-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/molecular-characteristics-and-antimicrobial-susceptibility-profiles--2-peer-reviewed-fulltext-article-IDR |
| _version_ | 1797335805778198528 |
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| author | Cao S Jiang X Suo J Lu Y Ju M Zeng Q Zheng Q Zhang Z Tang W |
| author_facet | Cao S Jiang X Suo J Lu Y Ju M Zeng Q Zheng Q Zhang Z Tang W |
| author_sort | Cao S |
| collection | DOAJ |
| description | Shuaijun Cao,1,* Xiaoying Jiang,2,* Jinshan Suo,3 Yanyan Lu,2 Mohan Ju,2 Qixiang Zeng,1 Qingru Zheng,1 Zuoyan Zhang,1 Wenqi Tang1 1Department of Critical Care Medicine, Shanghai Sixth People’s Hospital, Shanghai, People’s Republic of China; 2Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China; 3Department of Ophthalmology, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zuoyan Zhang; Wenqi Tang, Department of Critical Care Medicine, Shanghai Sixth People’s Hospital, No. 600 Yishan Road, Shanghai, 200000, People’s Republic of China, Tel +86 021-24058331, Email 18930177049@189.cn; zhang_zy1969@163.comIntroduction: Carbapenem-Resistant Enterobacteriaceae (CRE) has posed a significant threat to humans.The aim of this study was to investigate the molecular characteristics of blaKPC-producing Escherichia coli in a university-affiliated tertiary hospital.Methods: Polymerase chain reaction (PCR) and BLAST+ software were used to detect the prevalence of blaKPC in E. coli and Klebsiella pneumoniae. Whole-genome sequencing was performed for the blaKPC-harboring clinical E. coli isolates. Antimicrobial resistance genes, MLSTs, KPC-carrying plasmid typing and genetic environment of blaKPC were analyzed. A maximum likelihood core single nucleotide polymorphism (SNP)-based phylogeny tree was constructed to determine the evolutionary relationships within this ST131 collection. Conjugation experiments were performed to determine the mobilization of blaKPC. The minimal inhibitory concentrations of the common antimicrobial agents were determined using the broth microdilution method.Results: The prevalence of blaKPC in 424 clinical E. coli isolates and 1636 E. coli strains from GenBank database were 2.2% (45/2060) whereas the detection rate of blaKPC in K. pneumoniae from the GenBank database was 29.8% (415/1394). The blaKPC-harboring conjugants exhibited resistance to multiple β-lactams, except for cefepime-zidebactam and ceftazidime-avibactam. All blaKPC-carring E. coli isolates were susceptible to tigecycline and polymyxin B. ST131 was the dominant sequence type of blaKPC-carring E. coli, accounting for 40.0% (18/45). Most of the blaKPC-producing ST131 E. coli (89.5%,17/19) belonged to clade C ST131 lineage. Genetic environment analysis revealed that 57.8% (26/45) of blaKPC gene was linked to Tn 4401-associated structure ISKpn6-blaKPC-ISKpn7. IncN was the most common plasmid type in KPC-producing E. coli whereas IncFII was the dominant plasmid type in KPC-producing K. pneumoniae.Conclusion: The detection rate of blaKPC was lower in E. coli compared with K. pneumoniae. The dominant sequence and plasmid types of blaKPC-harboring isolates differed between E. coli and K. pneumoniae. Further studies about the role of the defense system in acquisition of KPC-plasmids in E. coli will be performed to provide new insights into the low prevalence of blaKPC.Keywords: Escherichia coli, blaKPC, carbapenemases, plasmids typing |
| first_indexed | 2024-03-08T08:43:43Z |
| format | Article |
| id | doaj.art-d12384376c784c1f8b9f65b8338258f5 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-03-08T08:43:43Z |
| publishDate | 2024-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-d12384376c784c1f8b9f65b8338258f52024-02-01T17:59:26ZengDove Medical PressInfection and Drug Resistance1178-69732024-01-01Volume 1731932789991Molecular Characteristics and Antimicrobial Susceptibility Profiles of blaKPC-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, ChinaCao SJiang XSuo JLu YJu MZeng QZheng QZhang ZTang WShuaijun Cao,1,* Xiaoying Jiang,2,* Jinshan Suo,3 Yanyan Lu,2 Mohan Ju,2 Qixiang Zeng,1 Qingru Zheng,1 Zuoyan Zhang,1 Wenqi Tang1 1Department of Critical Care Medicine, Shanghai Sixth People’s Hospital, Shanghai, People’s Republic of China; 2Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China; 3Department of Ophthalmology, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zuoyan Zhang; Wenqi Tang, Department of Critical Care Medicine, Shanghai Sixth People’s Hospital, No. 600 Yishan Road, Shanghai, 200000, People’s Republic of China, Tel +86 021-24058331, Email 18930177049@189.cn; zhang_zy1969@163.comIntroduction: Carbapenem-Resistant Enterobacteriaceae (CRE) has posed a significant threat to humans.The aim of this study was to investigate the molecular characteristics of blaKPC-producing Escherichia coli in a university-affiliated tertiary hospital.Methods: Polymerase chain reaction (PCR) and BLAST+ software were used to detect the prevalence of blaKPC in E. coli and Klebsiella pneumoniae. Whole-genome sequencing was performed for the blaKPC-harboring clinical E. coli isolates. Antimicrobial resistance genes, MLSTs, KPC-carrying plasmid typing and genetic environment of blaKPC were analyzed. A maximum likelihood core single nucleotide polymorphism (SNP)-based phylogeny tree was constructed to determine the evolutionary relationships within this ST131 collection. Conjugation experiments were performed to determine the mobilization of blaKPC. The minimal inhibitory concentrations of the common antimicrobial agents were determined using the broth microdilution method.Results: The prevalence of blaKPC in 424 clinical E. coli isolates and 1636 E. coli strains from GenBank database were 2.2% (45/2060) whereas the detection rate of blaKPC in K. pneumoniae from the GenBank database was 29.8% (415/1394). The blaKPC-harboring conjugants exhibited resistance to multiple β-lactams, except for cefepime-zidebactam and ceftazidime-avibactam. All blaKPC-carring E. coli isolates were susceptible to tigecycline and polymyxin B. ST131 was the dominant sequence type of blaKPC-carring E. coli, accounting for 40.0% (18/45). Most of the blaKPC-producing ST131 E. coli (89.5%,17/19) belonged to clade C ST131 lineage. Genetic environment analysis revealed that 57.8% (26/45) of blaKPC gene was linked to Tn 4401-associated structure ISKpn6-blaKPC-ISKpn7. IncN was the most common plasmid type in KPC-producing E. coli whereas IncFII was the dominant plasmid type in KPC-producing K. pneumoniae.Conclusion: The detection rate of blaKPC was lower in E. coli compared with K. pneumoniae. The dominant sequence and plasmid types of blaKPC-harboring isolates differed between E. coli and K. pneumoniae. Further studies about the role of the defense system in acquisition of KPC-plasmids in E. coli will be performed to provide new insights into the low prevalence of blaKPC.Keywords: Escherichia coli, blaKPC, carbapenemases, plasmids typinghttps://www.dovepress.com/molecular-characteristics-and-antimicrobial-susceptibility-profiles--2-peer-reviewed-fulltext-article-IDRescherichia coliblakpccarbapenemasesplasmids typing |
| spellingShingle | Cao S Jiang X Suo J Lu Y Ju M Zeng Q Zheng Q Zhang Z Tang W Molecular Characteristics and Antimicrobial Susceptibility Profiles of blaKPC-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, China Infection and Drug Resistance escherichia coli blakpc carbapenemases plasmids typing |
| title | Molecular Characteristics and Antimicrobial Susceptibility Profiles of blaKPC-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, China |
| title_full | Molecular Characteristics and Antimicrobial Susceptibility Profiles of blaKPC-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, China |
| title_fullStr | Molecular Characteristics and Antimicrobial Susceptibility Profiles of blaKPC-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, China |
| title_full_unstemmed | Molecular Characteristics and Antimicrobial Susceptibility Profiles of blaKPC-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, China |
| title_short | Molecular Characteristics and Antimicrobial Susceptibility Profiles of blaKPC-Producing Escherichia Coli Isolated from a Teaching Hospital in Shanghai, China |
| title_sort | molecular characteristics and antimicrobial susceptibility profiles of blakpc producing escherichia coli isolated from a teaching hospital in shanghai china |
| topic | escherichia coli blakpc carbapenemases plasmids typing |
| url | https://www.dovepress.com/molecular-characteristics-and-antimicrobial-susceptibility-profiles--2-peer-reviewed-fulltext-article-IDR |
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