Human olfactory neural progenitor cells reveal differences in IL-6, IL-8, thrombospondin-1, and MCP-1 in major depression disorder and borderline personality disorder
BackgroundDiscovering biological markers is essential for understanding and treating mental disorders. Despite the limitations of current non-invasive methods, neural progenitor cells from the olfactory epithelium (hNPCs-OE) have been emphasized as potential biomarker sources. This study measured so...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Psychiatry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpsyt.2024.1283406/full |
| _version_ | 1797217063859650560 |
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| author | Alan Patrick Davalos-Guzman Francisco Javier Vegas-Rodriguez Gerardo Bernabe Ramirez-Rodriguez Monica Flores-Ramos Perla Vanessa Romero-Luevano Jorge Julio Gonzalez-Olvera Ricardo Arturo Saracco-Alvarez |
| author_facet | Alan Patrick Davalos-Guzman Francisco Javier Vegas-Rodriguez Gerardo Bernabe Ramirez-Rodriguez Monica Flores-Ramos Perla Vanessa Romero-Luevano Jorge Julio Gonzalez-Olvera Ricardo Arturo Saracco-Alvarez |
| author_sort | Alan Patrick Davalos-Guzman |
| collection | DOAJ |
| description | BackgroundDiscovering biological markers is essential for understanding and treating mental disorders. Despite the limitations of current non-invasive methods, neural progenitor cells from the olfactory epithelium (hNPCs-OE) have been emphasized as potential biomarker sources. This study measured soluble factors in these cells in Major Depressive Disorder (MDD), Borderline Personality Disorder (BPD), and healthy controls (HC).MethodsWe assessed thirty-five participants divided into MDD (n=14), BPD (n=14), and HC (n=7). MDD was assessed using the Hamilton Depression Rating Scale. BPD was evaluated using the DSM-5 criteria and the Structured Clinical Interview for Personality Disorders. We isolated hNPCs-OE, collected intracellular proteins and conditioned medium, and quantified markers and soluble factors, including Interleukin-6, interleukin-8, and others. Analysis was conducted using one-way ANOVA or Kruskal-Wallis test and linear regression.ResultsWe found that hNPCs-OE of MDD and BPD decreased Sox2 and laminin receptor-67 kDa levels. MASH-1 decreased in BPD, while tubulin beta-III decreased in MDD compared to controls and BPD. Also, we found significant differences in IL-6, IL-8, MCP-1, and thrombospondin-1 levels between controls and MDD, or BPD, but not between MDD and BPD.ConclusionsAltered protein markers are evident in the nhNPCs-OE in MDD and BPD patients. These cells also secrete higher concentrations of inflammatory cytokines than HC cells. The results suggest the potential utility of hNPCs-OE as an in vitro model for researching biological protein markers in psychiatric disorders. However, more extensive validation studies are needed to confirm their effectiveness and specificity in neuropsychiatric disorders. |
| first_indexed | 2024-04-24T11:55:54Z |
| format | Article |
| id | doaj.art-d124c64da8c049028a6174859a5572fe |
| institution | Directory Open Access Journal |
| issn | 1664-0640 |
| language | English |
| last_indexed | 2024-04-24T11:55:54Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Psychiatry |
| spelling | doaj.art-d124c64da8c049028a6174859a5572fe2024-04-09T04:53:02ZengFrontiers Media S.A.Frontiers in Psychiatry1664-06402024-04-011510.3389/fpsyt.2024.12834061283406Human olfactory neural progenitor cells reveal differences in IL-6, IL-8, thrombospondin-1, and MCP-1 in major depression disorder and borderline personality disorderAlan Patrick Davalos-Guzman0Francisco Javier Vegas-Rodriguez1Gerardo Bernabe Ramirez-Rodriguez2Monica Flores-Ramos3Perla Vanessa Romero-Luevano4Jorge Julio Gonzalez-Olvera5Ricardo Arturo Saracco-Alvarez6Laboratorio de Neurogénesis, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Ciudad de México, MexicoLaboratorio de Neurogénesis, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Ciudad de México, MexicoLaboratorio de Neurogénesis, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Ciudad de México, MexicoSubdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría “Ramón de la Fuente Muñiz”, Ciudad de México, MexicoLaboratorio de Neurogénesis, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Ciudad de México, MexicoSubdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría “Ramón de la Fuente Muñiz”, Ciudad de México, MexicoSubdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría “Ramón de la Fuente Muñiz”, Ciudad de México, MexicoBackgroundDiscovering biological markers is essential for understanding and treating mental disorders. Despite the limitations of current non-invasive methods, neural progenitor cells from the olfactory epithelium (hNPCs-OE) have been emphasized as potential biomarker sources. This study measured soluble factors in these cells in Major Depressive Disorder (MDD), Borderline Personality Disorder (BPD), and healthy controls (HC).MethodsWe assessed thirty-five participants divided into MDD (n=14), BPD (n=14), and HC (n=7). MDD was assessed using the Hamilton Depression Rating Scale. BPD was evaluated using the DSM-5 criteria and the Structured Clinical Interview for Personality Disorders. We isolated hNPCs-OE, collected intracellular proteins and conditioned medium, and quantified markers and soluble factors, including Interleukin-6, interleukin-8, and others. Analysis was conducted using one-way ANOVA or Kruskal-Wallis test and linear regression.ResultsWe found that hNPCs-OE of MDD and BPD decreased Sox2 and laminin receptor-67 kDa levels. MASH-1 decreased in BPD, while tubulin beta-III decreased in MDD compared to controls and BPD. Also, we found significant differences in IL-6, IL-8, MCP-1, and thrombospondin-1 levels between controls and MDD, or BPD, but not between MDD and BPD.ConclusionsAltered protein markers are evident in the nhNPCs-OE in MDD and BPD patients. These cells also secrete higher concentrations of inflammatory cytokines than HC cells. The results suggest the potential utility of hNPCs-OE as an in vitro model for researching biological protein markers in psychiatric disorders. However, more extensive validation studies are needed to confirm their effectiveness and specificity in neuropsychiatric disorders.https://www.frontiersin.org/articles/10.3389/fpsyt.2024.1283406/fullmajor depressionborderline personality disorderolfactory epitheliumadult neurogenesisprogenitor cellssoluble factors |
| spellingShingle | Alan Patrick Davalos-Guzman Francisco Javier Vegas-Rodriguez Gerardo Bernabe Ramirez-Rodriguez Monica Flores-Ramos Perla Vanessa Romero-Luevano Jorge Julio Gonzalez-Olvera Ricardo Arturo Saracco-Alvarez Human olfactory neural progenitor cells reveal differences in IL-6, IL-8, thrombospondin-1, and MCP-1 in major depression disorder and borderline personality disorder Frontiers in Psychiatry major depression borderline personality disorder olfactory epithelium adult neurogenesis progenitor cells soluble factors |
| title | Human olfactory neural progenitor cells reveal differences in IL-6, IL-8, thrombospondin-1, and MCP-1 in major depression disorder and borderline personality disorder |
| title_full | Human olfactory neural progenitor cells reveal differences in IL-6, IL-8, thrombospondin-1, and MCP-1 in major depression disorder and borderline personality disorder |
| title_fullStr | Human olfactory neural progenitor cells reveal differences in IL-6, IL-8, thrombospondin-1, and MCP-1 in major depression disorder and borderline personality disorder |
| title_full_unstemmed | Human olfactory neural progenitor cells reveal differences in IL-6, IL-8, thrombospondin-1, and MCP-1 in major depression disorder and borderline personality disorder |
| title_short | Human olfactory neural progenitor cells reveal differences in IL-6, IL-8, thrombospondin-1, and MCP-1 in major depression disorder and borderline personality disorder |
| title_sort | human olfactory neural progenitor cells reveal differences in il 6 il 8 thrombospondin 1 and mcp 1 in major depression disorder and borderline personality disorder |
| topic | major depression borderline personality disorder olfactory epithelium adult neurogenesis progenitor cells soluble factors |
| url | https://www.frontiersin.org/articles/10.3389/fpsyt.2024.1283406/full |
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