Non-invasive skin sampling detects systemically administered drugs in humans
Clinical testing typically relies on invasive blood draws and biopsies. Alternative methods of sample collection are continually being developed to improve patient experience; swabbing the skin is one of the least invasive sampling methods possible. To show that skin swabs in combination with untarg...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321436/?tool=EBI |
| _version_ | 1818017264761307136 |
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| author | Morgan Panitchpakdi Kelly C. Weldon Alan K. Jarmusch Emily C. Gentry Arianna Choi Yadira Sepulveda Shaden Aguirre Kunyang Sun Jeremiah D. Momper Pieter C. Dorrestein Shirley M. Tsunoda |
| author_facet | Morgan Panitchpakdi Kelly C. Weldon Alan K. Jarmusch Emily C. Gentry Arianna Choi Yadira Sepulveda Shaden Aguirre Kunyang Sun Jeremiah D. Momper Pieter C. Dorrestein Shirley M. Tsunoda |
| author_sort | Morgan Panitchpakdi |
| collection | DOAJ |
| description | Clinical testing typically relies on invasive blood draws and biopsies. Alternative methods of sample collection are continually being developed to improve patient experience; swabbing the skin is one of the least invasive sampling methods possible. To show that skin swabs in combination with untargeted mass spectrometry (metabolomics) can be used for non-invasive monitoring of an oral drug, we report the kinetics and metabolism of diphenhydramine in healthy volunteers (n = 10) over the course of 24 hours in blood and three regions of the skin. Diphenhydramine and its metabolites were observed on the skin after peak plasma levels, varying by compound and skin location, and is an illustrative example of how systemically administered molecules can be detected on the skin surface. The observation of diphenhydramine directly from the skin supports the hypothesis that both parent drug and metabolites can be qualitatively measured from a simple non-invasive swab of the skin surface. The mechanism of the drug and metabolites pathway to the skin’s surface remains unknown. |
| first_indexed | 2024-04-14T07:23:54Z |
| format | Article |
| id | doaj.art-d127b390ce8e45eb868dec29462c4c88 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T07:23:54Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d127b390ce8e45eb868dec29462c4c882022-12-22T02:06:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01177Non-invasive skin sampling detects systemically administered drugs in humansMorgan PanitchpakdiKelly C. WeldonAlan K. JarmuschEmily C. GentryArianna ChoiYadira SepulvedaShaden AguirreKunyang SunJeremiah D. MomperPieter C. DorresteinShirley M. TsunodaClinical testing typically relies on invasive blood draws and biopsies. Alternative methods of sample collection are continually being developed to improve patient experience; swabbing the skin is one of the least invasive sampling methods possible. To show that skin swabs in combination with untargeted mass spectrometry (metabolomics) can be used for non-invasive monitoring of an oral drug, we report the kinetics and metabolism of diphenhydramine in healthy volunteers (n = 10) over the course of 24 hours in blood and three regions of the skin. Diphenhydramine and its metabolites were observed on the skin after peak plasma levels, varying by compound and skin location, and is an illustrative example of how systemically administered molecules can be detected on the skin surface. The observation of diphenhydramine directly from the skin supports the hypothesis that both parent drug and metabolites can be qualitatively measured from a simple non-invasive swab of the skin surface. The mechanism of the drug and metabolites pathway to the skin’s surface remains unknown.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321436/?tool=EBI |
| spellingShingle | Morgan Panitchpakdi Kelly C. Weldon Alan K. Jarmusch Emily C. Gentry Arianna Choi Yadira Sepulveda Shaden Aguirre Kunyang Sun Jeremiah D. Momper Pieter C. Dorrestein Shirley M. Tsunoda Non-invasive skin sampling detects systemically administered drugs in humans PLoS ONE |
| title | Non-invasive skin sampling detects systemically administered drugs in humans |
| title_full | Non-invasive skin sampling detects systemically administered drugs in humans |
| title_fullStr | Non-invasive skin sampling detects systemically administered drugs in humans |
| title_full_unstemmed | Non-invasive skin sampling detects systemically administered drugs in humans |
| title_short | Non-invasive skin sampling detects systemically administered drugs in humans |
| title_sort | non invasive skin sampling detects systemically administered drugs in humans |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321436/?tool=EBI |
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