Dynamics of cell rounding during detachment
Summary: Animal cells undergo repeated shape changes, for example by rounding up and respreading as they divide. Cell rounding can be also observed in interphase cells, for example when cancer cells switch from a mesenchymal to an ameboid mode of cell migration. Nevertheless, it remains unclear how...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-05-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223007733 |
| _version_ | 1797835707342913536 |
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| author | Agata Nyga Katarzyna Plak Martin Kräter Marta Urbanska Kyoohyun Kim Jochen Guck Buzz Baum |
| author_facet | Agata Nyga Katarzyna Plak Martin Kräter Marta Urbanska Kyoohyun Kim Jochen Guck Buzz Baum |
| author_sort | Agata Nyga |
| collection | DOAJ |
| description | Summary: Animal cells undergo repeated shape changes, for example by rounding up and respreading as they divide. Cell rounding can be also observed in interphase cells, for example when cancer cells switch from a mesenchymal to an ameboid mode of cell migration. Nevertheless, it remains unclear how interphase cells round up. In this article, we demonstrate that a partial loss of substrate adhesion triggers actomyosin-dependent cortical remodeling and ERM activation, which facilitates further adhesion loss causing cells to round. Although the path of rounding in this case superficially resembles mitotic rounding in involving ERM phosphorylation, retraction fiber formation, and cortical remodeling downstream of ROCK, it does not require Ect2. This work provides insights into the way partial loss of adhesion actives cortical remodeling to drive cell detachment from the substrate. This is important to consider when studying the mechanics of cells in suspension, for example using methods like real-time deformability cytometry (RT-DC). |
| first_indexed | 2024-04-09T14:57:55Z |
| format | Article |
| id | doaj.art-d129827eb7524fbdb521520b5a1fbf80 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T14:57:55Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-d129827eb7524fbdb521520b5a1fbf802023-05-02T04:05:14ZengElsevieriScience2589-00422023-05-01265106696Dynamics of cell rounding during detachmentAgata Nyga0Katarzyna Plak1Martin Kräter2Marta Urbanska3Kyoohyun Kim4Jochen Guck5Buzz Baum6Cell Biology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UKMRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK; Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, 01307 Dresden, GermanyBiotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, 01307 Dresden, Germany; Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, GermanyBiotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, 01307 Dresden, Germany; Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, GermanyBiotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, 01307 Dresden, Germany; Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, GermanyBiotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, 01307 Dresden, Germany; Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, GermanyCell Biology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK; Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UK; Corresponding authorSummary: Animal cells undergo repeated shape changes, for example by rounding up and respreading as they divide. Cell rounding can be also observed in interphase cells, for example when cancer cells switch from a mesenchymal to an ameboid mode of cell migration. Nevertheless, it remains unclear how interphase cells round up. In this article, we demonstrate that a partial loss of substrate adhesion triggers actomyosin-dependent cortical remodeling and ERM activation, which facilitates further adhesion loss causing cells to round. Although the path of rounding in this case superficially resembles mitotic rounding in involving ERM phosphorylation, retraction fiber formation, and cortical remodeling downstream of ROCK, it does not require Ect2. This work provides insights into the way partial loss of adhesion actives cortical remodeling to drive cell detachment from the substrate. This is important to consider when studying the mechanics of cells in suspension, for example using methods like real-time deformability cytometry (RT-DC).http://www.sciencedirect.com/science/article/pii/S2589004223007733Molecular biologyCell biologyDevelopmental biology |
| spellingShingle | Agata Nyga Katarzyna Plak Martin Kräter Marta Urbanska Kyoohyun Kim Jochen Guck Buzz Baum Dynamics of cell rounding during detachment iScience Molecular biology Cell biology Developmental biology |
| title | Dynamics of cell rounding during detachment |
| title_full | Dynamics of cell rounding during detachment |
| title_fullStr | Dynamics of cell rounding during detachment |
| title_full_unstemmed | Dynamics of cell rounding during detachment |
| title_short | Dynamics of cell rounding during detachment |
| title_sort | dynamics of cell rounding during detachment |
| topic | Molecular biology Cell biology Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223007733 |
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