Function and Gene Expression of Islets Experimentally Transplanted to Muscle and Omentum
Islet transplantation to the liver is a potential curative treatment for patients with type 1 diabetes. Muscle and the greater omentum are two alternative implantation sites, which can provide excellent engraftment and hold potential as future sites for stem-cell-derived beta-cell replacement. We ev...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2020-11-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/0963689720960184 |
| _version_ | 1818922270974803968 |
|---|---|
| author | Daniel Espes Hanna Liljebäck Petra Franzén My Quach Joey Lau Per-Ola Carlsson |
| author_facet | Daniel Espes Hanna Liljebäck Petra Franzén My Quach Joey Lau Per-Ola Carlsson |
| author_sort | Daniel Espes |
| collection | DOAJ |
| description | Islet transplantation to the liver is a potential curative treatment for patients with type 1 diabetes. Muscle and the greater omentum are two alternative implantation sites, which can provide excellent engraftment and hold potential as future sites for stem-cell-derived beta-cell replacement. We evaluated the functional outcome after islet transplantation to muscle and omentum and found that alloxan-diabetic animals were cured with a low number of islets (200) at both sites. The cured animals had a normal area under the curve blood glucose response to intravenous glucose, albeit animals with intramuscular islet grafts had increased 120-min blood glucose levels. They also demonstrated an exaggerated counter regulatory response to hypoglycemia. The expression of genes important for beta-cell function was, at both implantation sites, comparable to that in native pancreatic islets. The gene expression of insulin (INS1 and INS2) and glucose transporter-2 was even increased, and the expression of lactate dehydrogenase decreased, at both sites when compared to native islets. We conclude that muscle and omentum provide excellent conditions for engraftment of transplanted islets. When compared to control, 200 islets implanted to the omentum displayed a restored glucose tolerance, whereas animals with intramuscular islet grafts of similar size displayed mild glucose intolerance. |
| first_indexed | 2024-12-20T01:50:53Z |
| format | Article |
| id | doaj.art-d12c0120257d4d70a4092e45fb3a8624 |
| institution | Directory Open Access Journal |
| issn | 1555-3892 |
| language | English |
| last_indexed | 2024-12-20T01:50:53Z |
| publishDate | 2020-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-d12c0120257d4d70a4092e45fb3a86242022-12-21T19:57:39ZengSAGE PublishingCell Transplantation1555-38922020-11-012910.1177/0963689720960184Function and Gene Expression of Islets Experimentally Transplanted to Muscle and OmentumDaniel Espes0Hanna Liljebäck1Petra Franzén2My Quach3Joey Lau4Per-Ola Carlsson5 Department of Medical Sciences, Uppsala University, Uppsala, Sweden Department of Medical Sciences, Uppsala University, Uppsala, Sweden Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden Department of Medical Sciences, Uppsala University, Uppsala, SwedenIslet transplantation to the liver is a potential curative treatment for patients with type 1 diabetes. Muscle and the greater omentum are two alternative implantation sites, which can provide excellent engraftment and hold potential as future sites for stem-cell-derived beta-cell replacement. We evaluated the functional outcome after islet transplantation to muscle and omentum and found that alloxan-diabetic animals were cured with a low number of islets (200) at both sites. The cured animals had a normal area under the curve blood glucose response to intravenous glucose, albeit animals with intramuscular islet grafts had increased 120-min blood glucose levels. They also demonstrated an exaggerated counter regulatory response to hypoglycemia. The expression of genes important for beta-cell function was, at both implantation sites, comparable to that in native pancreatic islets. The gene expression of insulin (INS1 and INS2) and glucose transporter-2 was even increased, and the expression of lactate dehydrogenase decreased, at both sites when compared to native islets. We conclude that muscle and omentum provide excellent conditions for engraftment of transplanted islets. When compared to control, 200 islets implanted to the omentum displayed a restored glucose tolerance, whereas animals with intramuscular islet grafts of similar size displayed mild glucose intolerance.https://doi.org/10.1177/0963689720960184 |
| spellingShingle | Daniel Espes Hanna Liljebäck Petra Franzén My Quach Joey Lau Per-Ola Carlsson Function and Gene Expression of Islets Experimentally Transplanted to Muscle and Omentum Cell Transplantation |
| title | Function and Gene Expression of Islets Experimentally Transplanted to Muscle and Omentum |
| title_full | Function and Gene Expression of Islets Experimentally Transplanted to Muscle and Omentum |
| title_fullStr | Function and Gene Expression of Islets Experimentally Transplanted to Muscle and Omentum |
| title_full_unstemmed | Function and Gene Expression of Islets Experimentally Transplanted to Muscle and Omentum |
| title_short | Function and Gene Expression of Islets Experimentally Transplanted to Muscle and Omentum |
| title_sort | function and gene expression of islets experimentally transplanted to muscle and omentum |
| url | https://doi.org/10.1177/0963689720960184 |
| work_keys_str_mv | AT danielespes functionandgeneexpressionofisletsexperimentallytransplantedtomuscleandomentum AT hannaliljeback functionandgeneexpressionofisletsexperimentallytransplantedtomuscleandomentum AT petrafranzen functionandgeneexpressionofisletsexperimentallytransplantedtomuscleandomentum AT myquach functionandgeneexpressionofisletsexperimentallytransplantedtomuscleandomentum AT joeylau functionandgeneexpressionofisletsexperimentallytransplantedtomuscleandomentum AT perolacarlsson functionandgeneexpressionofisletsexperimentallytransplantedtomuscleandomentum |