Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors
Background/Aim: A number of biologically active substances were proved as an alternative to conventional anticancer medicines. The aim of the study is in vitro investigation of the anticancer activity of mono- and di-Rhamnolipids (RL-1 and RL-2) against human breast cancer. Additionally, the combina...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2297-8739/8/7/92 |
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| author | Severina Semkova Georgi Antov Ivan Iliev Iana Tsoneva Pavel Lefterov Nelly Christova Lilyana Nacheva Ivanka Stoineva Lyudmila Kabaivanova Galya Staneva Biliana Nikolova |
| author_facet | Severina Semkova Georgi Antov Ivan Iliev Iana Tsoneva Pavel Lefterov Nelly Christova Lilyana Nacheva Ivanka Stoineva Lyudmila Kabaivanova Galya Staneva Biliana Nikolova |
| author_sort | Severina Semkova |
| collection | DOAJ |
| description | Background/Aim: A number of biologically active substances were proved as an alternative to conventional anticancer medicines. The aim of the study is in vitro investigation of the anticancer activity of mono- and di-Rhamnolipids (RL-1 and RL-2) against human breast cancer. Additionally, the combination with Cisplatin was analyzed. Materials and Methods: Breast cell lines (MCF-10A, MCF-7 and MDA-MB-231) were treated with RLs and in combination with Cisplatin. The viability was analyzed using MTT assay, and investigation of autophagy was performed via acridine orange staining. Results: In contrast to the healthy cells, both tested cancer lines exhibited sensitivity to RLs treatment. This effect was accompanied by an influence on the autophagy-related acidic formation process. Only for the triple-negative breast cancer cell line (MDA-MB-231) the synergistic effect of the combined treatment (10 µM Cisplatin and 1 µg/mL RL-2) was observed. Conclusion: Based on studies on the reorganization of membrane models in the presence of RL and the data about a higher amount of lipid rafts in cancer cell membranes than in non-tumorigenic, we suggest a possible mechanism of membrane remodelling by formation of endosomes. Shortly, in order to have a synergistic effect, it is necessary to have Cisplatin andRL-2 as RL2 is a molecule inducingpositive membrane curvature. |
| first_indexed | 2024-03-10T10:00:02Z |
| format | Article |
| id | doaj.art-d1357908b2194463a67cca1a0895e951 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T10:00:02Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-d1357908b2194463a67cca1a0895e9512023-11-22T02:04:27ZengMDPI AGSeparations2297-87392021-06-01879210.3390/separations8070092Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy InhibitorsSeverina Semkova0Georgi Antov1Ivan Iliev2Iana Tsoneva3Pavel Lefterov4Nelly Christova5Lilyana Nacheva6Ivanka Stoineva7Lyudmila Kabaivanova8Galya Staneva9Biliana Nikolova10Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 21, 1113 Sofia, BulgariaFaculty of Educational Studies and the Arts University of Sofia, Bul. Shipchenski Prohod, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 26, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 9, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 26, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str., Bl. 21, 1113 Sofia, BulgariaBackground/Aim: A number of biologically active substances were proved as an alternative to conventional anticancer medicines. The aim of the study is in vitro investigation of the anticancer activity of mono- and di-Rhamnolipids (RL-1 and RL-2) against human breast cancer. Additionally, the combination with Cisplatin was analyzed. Materials and Methods: Breast cell lines (MCF-10A, MCF-7 and MDA-MB-231) were treated with RLs and in combination with Cisplatin. The viability was analyzed using MTT assay, and investigation of autophagy was performed via acridine orange staining. Results: In contrast to the healthy cells, both tested cancer lines exhibited sensitivity to RLs treatment. This effect was accompanied by an influence on the autophagy-related acidic formation process. Only for the triple-negative breast cancer cell line (MDA-MB-231) the synergistic effect of the combined treatment (10 µM Cisplatin and 1 µg/mL RL-2) was observed. Conclusion: Based on studies on the reorganization of membrane models in the presence of RL and the data about a higher amount of lipid rafts in cancer cell membranes than in non-tumorigenic, we suggest a possible mechanism of membrane remodelling by formation of endosomes. Shortly, in order to have a synergistic effect, it is necessary to have Cisplatin andRL-2 as RL2 is a molecule inducingpositive membrane curvature.https://www.mdpi.com/2297-8739/8/7/92bacterial natural rhamnolipidsbreast cancerautophagycytotoxitymembrane reorganizationendocytosis |
| spellingShingle | Severina Semkova Georgi Antov Ivan Iliev Iana Tsoneva Pavel Lefterov Nelly Christova Lilyana Nacheva Ivanka Stoineva Lyudmila Kabaivanova Galya Staneva Biliana Nikolova Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors Separations bacterial natural rhamnolipids breast cancer autophagy cytotoxity membrane reorganization endocytosis |
| title | Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors |
| title_full | Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors |
| title_fullStr | Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors |
| title_full_unstemmed | Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors |
| title_short | Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors |
| title_sort | rhamnolipid biosurfactants possible natural anticancer agents and autophagy inhibitors |
| topic | bacterial natural rhamnolipids breast cancer autophagy cytotoxity membrane reorganization endocytosis |
| url | https://www.mdpi.com/2297-8739/8/7/92 |
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