A Histological Evaluation of Adventitious Bud Formation in Cotyledons in Crotalaria juncea L.
In Crotalaria juncea L., adventitious buds were formed in cotyledonary expiants cultured on 0.8% agar-solidified 1/2 MS basal medium containing B5 vitamins, 1.0 or 0.5 mg L–1 NAA, 5.0 or 10.0 mg L–1 BA and 3% sucrose. The frequency of adventitious bud formation was 30-45% in all combinations of NAA...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2002-01-01
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| Series: | Plant Production Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1626/pps.5.301 |
| _version_ | 1818760227024011264 |
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| author | Hiroyuki Daimon Hitoshi Ohno Yoko Akasaka Masahiro Mii |
| author_facet | Hiroyuki Daimon Hitoshi Ohno Yoko Akasaka Masahiro Mii |
| author_sort | Hiroyuki Daimon |
| collection | DOAJ |
| description | In Crotalaria juncea L., adventitious buds were formed in cotyledonary expiants cultured on 0.8% agar-solidified 1/2 MS basal medium containing B5 vitamins, 1.0 or 0.5 mg L–1 NAA, 5.0 or 10.0 mg L–1 BA and 3% sucrose. The frequency of adventitious bud formation was 30-45% in all combinations of NAA and BA. In histological observations, prominent mitotic figures were observed in several cells of the subepidermal palisade layers on the adaxial side of the expiants in contact with medium after 3 days of culture. Calli were formed within 6 days of culture. After 10 days of culture, numerous mature tracheary elements were produced at random in the proliferated regions, and cell divisions at the superficial region led to the formation of the meristematic structure. The shoot apex of the seedling produced numerous trichomes from superficial cells, but the adventitious bud formed on the cotyledon produced no trichomes. Initiation of the meristematic region in the expiant could be used as a target site for gene transfer experiments. |
| first_indexed | 2024-12-18T06:55:16Z |
| format | Article |
| id | doaj.art-d136977932c344ad8582aafa263b603a |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-12-18T06:55:16Z |
| publishDate | 2002-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Production Science |
| spelling | doaj.art-d136977932c344ad8582aafa263b603a2022-12-21T21:17:12ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10082002-01-015430130410.1626/pps.5.30111644550A Histological Evaluation of Adventitious Bud Formation in Cotyledons in Crotalaria juncea L.Hiroyuki Daimon0Hitoshi Ohno1Yoko Akasaka2Masahiro Mii3Osaka Prefecture UniversityOsaka Prefecture UniversityIbaraki Agricultural CenterChiba UniversityIn Crotalaria juncea L., adventitious buds were formed in cotyledonary expiants cultured on 0.8% agar-solidified 1/2 MS basal medium containing B5 vitamins, 1.0 or 0.5 mg L–1 NAA, 5.0 or 10.0 mg L–1 BA and 3% sucrose. The frequency of adventitious bud formation was 30-45% in all combinations of NAA and BA. In histological observations, prominent mitotic figures were observed in several cells of the subepidermal palisade layers on the adaxial side of the expiants in contact with medium after 3 days of culture. Calli were formed within 6 days of culture. After 10 days of culture, numerous mature tracheary elements were produced at random in the proliferated regions, and cell divisions at the superficial region led to the formation of the meristematic structure. The shoot apex of the seedling produced numerous trichomes from superficial cells, but the adventitious bud formed on the cotyledon produced no trichomes. Initiation of the meristematic region in the expiant could be used as a target site for gene transfer experiments.http://dx.doi.org/10.1626/pps.5.301Adventitious budCotyledonCrotalariaGreen manure cropShoot apexTissue culture |
| spellingShingle | Hiroyuki Daimon Hitoshi Ohno Yoko Akasaka Masahiro Mii A Histological Evaluation of Adventitious Bud Formation in Cotyledons in Crotalaria juncea L. Plant Production Science Adventitious bud Cotyledon Crotalaria Green manure crop Shoot apex Tissue culture |
| title | A Histological Evaluation of Adventitious Bud Formation in Cotyledons in Crotalaria juncea L. |
| title_full | A Histological Evaluation of Adventitious Bud Formation in Cotyledons in Crotalaria juncea L. |
| title_fullStr | A Histological Evaluation of Adventitious Bud Formation in Cotyledons in Crotalaria juncea L. |
| title_full_unstemmed | A Histological Evaluation of Adventitious Bud Formation in Cotyledons in Crotalaria juncea L. |
| title_short | A Histological Evaluation of Adventitious Bud Formation in Cotyledons in Crotalaria juncea L. |
| title_sort | histological evaluation of adventitious bud formation in cotyledons in crotalaria juncea l |
| topic | Adventitious bud Cotyledon Crotalaria Green manure crop Shoot apex Tissue culture |
| url | http://dx.doi.org/10.1626/pps.5.301 |
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