Anti-Metastatic Activity of Tagitinin C from <i>Tithonia diversifolia</i> in a Xenograft <i>Mouse</i> Model of Hepatocellular Carcinoma
Sesquiterpenoid tagitinin C, present in <i>Tithonia diversifolia</i> leaves, has been known to have anti-hepatoma properties. Therefore, we investigated the anti-metastatic potential of tagitinin C in xenograft models of hepatocellular carcinoma (HCC). We isolated tagitinin C from a meth...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2673-4389/2/4/30 |
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| author | Chuan-Yi Lin May-Hua Liao Chi-Yu Yang Chao-Kai Chang Shih-Mei Hsu Chi-Long Juang Hsiao-Chuan Wen |
| author_facet | Chuan-Yi Lin May-Hua Liao Chi-Yu Yang Chao-Kai Chang Shih-Mei Hsu Chi-Long Juang Hsiao-Chuan Wen |
| author_sort | Chuan-Yi Lin |
| collection | DOAJ |
| description | Sesquiterpenoid tagitinin C, present in <i>Tithonia diversifolia</i> leaves, has been known to have anti-hepatoma properties. Therefore, we investigated the anti-metastatic potential of tagitinin C in xenograft models of hepatocellular carcinoma (HCC). We isolated tagitinin C from a methanolic extract of the leaves of <i>T. diversifolia</i>. HepG-2 and Huh 7 hepatoma cells were treated with tagitinin C, and cell viability, migration, and matrix metalloproteinase (MPP) activity were assessed using the 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide assay, scratch migration assay, and MMP activity assay, respectively. We used magnetic resonance spectroscopy to determine the tumorigenicity of xenografts inoculated with Hep-G2 and Huh 7 cells. Tagitinin C was cytotoxic against Hep-G2 and Huh 7 cells, with IC<sub>50</sub> values of 2.0 ± 0.1 µg/mL and 1.2 ± 0.1 µg/mL, respectively, and it showed an anti-metastatic effect in vitro. Additionally, MRS assays revealed that tagitinin C (15 g/mouse/day) reduced the tumorigenicity of Hep-G2 and Huh 7 cell xenografts. Tagitinin C demonstrated significant antitumor and anti-metastatic activity in the two human hepatoma cell lines. Tagitinin C might be used as an alternative or auxiliary therapy for the treatment of HCC, and its effect should be further investigated in clinical settings. |
| first_indexed | 2024-03-09T16:11:24Z |
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| language | English |
| last_indexed | 2024-03-09T16:11:24Z |
| publishDate | 2022-12-01 |
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| series | Livers |
| spelling | doaj.art-d136bb7f956749a7acf87fbd1ff0837e2023-11-24T16:14:34ZengMDPI AGLivers2673-43892022-12-012440041110.3390/livers2040030Anti-Metastatic Activity of Tagitinin C from <i>Tithonia diversifolia</i> in a Xenograft <i>Mouse</i> Model of Hepatocellular CarcinomaChuan-Yi Lin0May-Hua Liao1Chi-Yu Yang2Chao-Kai Chang3Shih-Mei Hsu4Chi-Long Juang5Hsiao-Chuan Wen6Department of Medical Imaging and Radiological Technology, Yuanpei University of Medical Technology, Hsinchu 30015, TaiwanDepartment of Nursing, Yuanpei University of Medical Technology, Hsinchu 30015, TaiwanAnimal Technology Laboratories, Agricultural Technology Research Institute, Miaoli 35053, TaiwanTaipei Nobel Eye Institute, Taipei 100, TaiwanDepartment of Medical Imaging and Radiological Technology, Yuanpei University of Medical Technology, Hsinchu 30015, TaiwanDepartment of Medical Imaging and Radiological Technology, Yuanpei University of Medical Technology, Hsinchu 30015, TaiwanDepartment of Pet Healthcare, Yuanpei University of Medical Technology, Hsinchu 30015, TaiwanSesquiterpenoid tagitinin C, present in <i>Tithonia diversifolia</i> leaves, has been known to have anti-hepatoma properties. Therefore, we investigated the anti-metastatic potential of tagitinin C in xenograft models of hepatocellular carcinoma (HCC). We isolated tagitinin C from a methanolic extract of the leaves of <i>T. diversifolia</i>. HepG-2 and Huh 7 hepatoma cells were treated with tagitinin C, and cell viability, migration, and matrix metalloproteinase (MPP) activity were assessed using the 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide assay, scratch migration assay, and MMP activity assay, respectively. We used magnetic resonance spectroscopy to determine the tumorigenicity of xenografts inoculated with Hep-G2 and Huh 7 cells. Tagitinin C was cytotoxic against Hep-G2 and Huh 7 cells, with IC<sub>50</sub> values of 2.0 ± 0.1 µg/mL and 1.2 ± 0.1 µg/mL, respectively, and it showed an anti-metastatic effect in vitro. Additionally, MRS assays revealed that tagitinin C (15 g/mouse/day) reduced the tumorigenicity of Hep-G2 and Huh 7 cell xenografts. Tagitinin C demonstrated significant antitumor and anti-metastatic activity in the two human hepatoma cell lines. Tagitinin C might be used as an alternative or auxiliary therapy for the treatment of HCC, and its effect should be further investigated in clinical settings.https://www.mdpi.com/2673-4389/2/4/30tagitinin Chepatocellular carcinomametastasis<i>Tithonia diversifolia</i>cancerxenograft model |
| spellingShingle | Chuan-Yi Lin May-Hua Liao Chi-Yu Yang Chao-Kai Chang Shih-Mei Hsu Chi-Long Juang Hsiao-Chuan Wen Anti-Metastatic Activity of Tagitinin C from <i>Tithonia diversifolia</i> in a Xenograft <i>Mouse</i> Model of Hepatocellular Carcinoma Livers tagitinin C hepatocellular carcinoma metastasis <i>Tithonia diversifolia</i> cancer xenograft model |
| title | Anti-Metastatic Activity of Tagitinin C from <i>Tithonia diversifolia</i> in a Xenograft <i>Mouse</i> Model of Hepatocellular Carcinoma |
| title_full | Anti-Metastatic Activity of Tagitinin C from <i>Tithonia diversifolia</i> in a Xenograft <i>Mouse</i> Model of Hepatocellular Carcinoma |
| title_fullStr | Anti-Metastatic Activity of Tagitinin C from <i>Tithonia diversifolia</i> in a Xenograft <i>Mouse</i> Model of Hepatocellular Carcinoma |
| title_full_unstemmed | Anti-Metastatic Activity of Tagitinin C from <i>Tithonia diversifolia</i> in a Xenograft <i>Mouse</i> Model of Hepatocellular Carcinoma |
| title_short | Anti-Metastatic Activity of Tagitinin C from <i>Tithonia diversifolia</i> in a Xenograft <i>Mouse</i> Model of Hepatocellular Carcinoma |
| title_sort | anti metastatic activity of tagitinin c from i tithonia diversifolia i in a xenograft i mouse i model of hepatocellular carcinoma |
| topic | tagitinin C hepatocellular carcinoma metastasis <i>Tithonia diversifolia</i> cancer xenograft model |
| url | https://www.mdpi.com/2673-4389/2/4/30 |
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