Low-volume label-free SARS-CoV-2 detection with the microcavity-based optical fiber sensor
Abstract Accurate and fast detection of viruses is crucial for controlling outbreaks of many diseases; therefore, to date, numerous sensing systems for their detection have been studied. On top of the performance of these sensing systems, the availability of biorecognition elements specific to espec...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-28790-y |
| _version_ | 1811175903276826624 |
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| author | Monika Janik Tomasz Gabler Marcin Koba Mirosława Panasiuk Yanina Dashkevich Tomasz Łęga Agnieszka Dąbrowska Antonina Naskalska Sabina Żołędowska Dawid Nidzworski Krzysztof Pyrć Beata Gromadzka Mateusz Śmietana |
| author_facet | Monika Janik Tomasz Gabler Marcin Koba Mirosława Panasiuk Yanina Dashkevich Tomasz Łęga Agnieszka Dąbrowska Antonina Naskalska Sabina Żołędowska Dawid Nidzworski Krzysztof Pyrć Beata Gromadzka Mateusz Śmietana |
| author_sort | Monika Janik |
| collection | DOAJ |
| description | Abstract Accurate and fast detection of viruses is crucial for controlling outbreaks of many diseases; therefore, to date, numerous sensing systems for their detection have been studied. On top of the performance of these sensing systems, the availability of biorecognition elements specific to especially the new etiological agents is an additional fundamental challenge. Therefore, besides high sensitivity and selectivity, such advantages as the size of the sensor and possibly low volume of analyzed samples are also important, especially at the stage of evaluating the receptor-target interactions in the case of new etiological agents when typically, only tiny amounts of the receptor are available for testing. This work introduces a real-time, highly miniaturized sensing solution based on microcavity in-line Mach–Zehnder interferometer (μIMZI) induced in optical fiber for SARS-CoV-2 virus-like particles detection. The assay is designed to detect conserved regions of the SARS-CoV-2 viral particles in a sample with a volume as small as hundreds of picoliters, reaching the detection limit at the single ng per mL level. |
| first_indexed | 2024-04-10T19:43:30Z |
| format | Article |
| id | doaj.art-d1405b8da10e4e00a3ac4208d209f6be |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T19:43:30Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d1405b8da10e4e00a3ac4208d209f6be2023-01-29T12:11:50ZengNature PortfolioScientific Reports2045-23222023-01-0113111010.1038/s41598-023-28790-yLow-volume label-free SARS-CoV-2 detection with the microcavity-based optical fiber sensorMonika Janik0Tomasz Gabler1Marcin Koba2Mirosława Panasiuk3Yanina Dashkevich4Tomasz Łęga5Agnieszka Dąbrowska6Antonina Naskalska7Sabina Żołędowska8Dawid Nidzworski9Krzysztof Pyrć10Beata Gromadzka11Mateusz Śmietana12Institute of Microelectronics and Optoelectronics, Warsaw University of TechnologyInstitute of Microelectronics and Optoelectronics, Warsaw University of TechnologyInstitute of Microelectronics and Optoelectronics, Warsaw University of TechnologyInstitute of Biotechnology and Molecular MedicineInstitute of Biotechnology and Molecular MedicineInstitute of Biotechnology and Molecular MedicineVirogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian UniversityVirogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian UniversityInstitute of Biotechnology and Molecular MedicineInstitute of Biotechnology and Molecular MedicineVirogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian UniversityInstitute of Biotechnology and Molecular MedicineInstitute of Microelectronics and Optoelectronics, Warsaw University of TechnologyAbstract Accurate and fast detection of viruses is crucial for controlling outbreaks of many diseases; therefore, to date, numerous sensing systems for their detection have been studied. On top of the performance of these sensing systems, the availability of biorecognition elements specific to especially the new etiological agents is an additional fundamental challenge. Therefore, besides high sensitivity and selectivity, such advantages as the size of the sensor and possibly low volume of analyzed samples are also important, especially at the stage of evaluating the receptor-target interactions in the case of new etiological agents when typically, only tiny amounts of the receptor are available for testing. This work introduces a real-time, highly miniaturized sensing solution based on microcavity in-line Mach–Zehnder interferometer (μIMZI) induced in optical fiber for SARS-CoV-2 virus-like particles detection. The assay is designed to detect conserved regions of the SARS-CoV-2 viral particles in a sample with a volume as small as hundreds of picoliters, reaching the detection limit at the single ng per mL level.https://doi.org/10.1038/s41598-023-28790-y |
| spellingShingle | Monika Janik Tomasz Gabler Marcin Koba Mirosława Panasiuk Yanina Dashkevich Tomasz Łęga Agnieszka Dąbrowska Antonina Naskalska Sabina Żołędowska Dawid Nidzworski Krzysztof Pyrć Beata Gromadzka Mateusz Śmietana Low-volume label-free SARS-CoV-2 detection with the microcavity-based optical fiber sensor Scientific Reports |
| title | Low-volume label-free SARS-CoV-2 detection with the microcavity-based optical fiber sensor |
| title_full | Low-volume label-free SARS-CoV-2 detection with the microcavity-based optical fiber sensor |
| title_fullStr | Low-volume label-free SARS-CoV-2 detection with the microcavity-based optical fiber sensor |
| title_full_unstemmed | Low-volume label-free SARS-CoV-2 detection with the microcavity-based optical fiber sensor |
| title_short | Low-volume label-free SARS-CoV-2 detection with the microcavity-based optical fiber sensor |
| title_sort | low volume label free sars cov 2 detection with the microcavity based optical fiber sensor |
| url | https://doi.org/10.1038/s41598-023-28790-y |
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