Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier <i>Magnetospirillum</i> sp. Strain 15-1
The facultative denitrifying alphaproteobacterium <i>Magnetospirillum</i> sp. strain 15-1 had been isolated from the hypoxic rhizosphere of a constructed wetland model fed with toluene. This bacterium can catabolize toluene anaerobically but not aerobically. Here, we used strain 15-1 to...
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MDPI AG
2020-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/5/681 |
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| author | Ingrid Meyer-Cifuentes Sylvie Gruhl Sven-Bastiaan Haange Vanessa Lünsmann Nico Jehmlich Martin von Bergen Hermann J. Heipieper Jochen A. Müller |
| author_facet | Ingrid Meyer-Cifuentes Sylvie Gruhl Sven-Bastiaan Haange Vanessa Lünsmann Nico Jehmlich Martin von Bergen Hermann J. Heipieper Jochen A. Müller |
| author_sort | Ingrid Meyer-Cifuentes |
| collection | DOAJ |
| description | The facultative denitrifying alphaproteobacterium <i>Magnetospirillum</i> sp. strain 15-1 had been isolated from the hypoxic rhizosphere of a constructed wetland model fed with toluene. This bacterium can catabolize toluene anaerobically but not aerobically. Here, we used strain 15-1 to investigate regulation of expression of the highly oxygen-sensitive glycyl radical enzyme benzylsuccinate synthase, which catalyzes the first step in anaerobic toluene degradation. In cells growing aerobically with benzoate, the addition of toluene resulted in a ~20-fold increased transcription of <i>bssA</i>, encoding for the catalytically active subunit of the enzyme. Under anoxic conditions, <i>bssA</i> mRNA copy numbers were up to 129-fold higher in cells growing with toluene as compared to cells growing with benzoate. Proteomics showed that abundance of benzylsuccinate synthase increased in cells growing anaerobically with toluene. In contrast, peptides of this enzyme were never detected in oxic conditions. These findings show that synthesis of benzylsuccinate synthase was under stringent post-transcriptional control in the presence of oxygen, which is a novel level of regulation for glycyl radical enzymes. |
| first_indexed | 2024-03-10T19:59:46Z |
| format | Article |
| id | doaj.art-d140e02713294464b40d845f2a2f1e7f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:59:46Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d140e02713294464b40d845f2a2f1e7f2023-11-19T23:43:46ZengMDPI AGMicroorganisms2076-26072020-05-018568110.3390/microorganisms8050681Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier <i>Magnetospirillum</i> sp. Strain 15-1Ingrid Meyer-Cifuentes0Sylvie Gruhl1Sven-Bastiaan Haange2Vanessa Lünsmann3Nico Jehmlich4Martin von Bergen5Hermann J. Heipieper6Jochen A. Müller7Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, GermanyDepartment of Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318 Leipzig, GermanyDepartment of Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, GermanyDepartment of Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, GermanyThe facultative denitrifying alphaproteobacterium <i>Magnetospirillum</i> sp. strain 15-1 had been isolated from the hypoxic rhizosphere of a constructed wetland model fed with toluene. This bacterium can catabolize toluene anaerobically but not aerobically. Here, we used strain 15-1 to investigate regulation of expression of the highly oxygen-sensitive glycyl radical enzyme benzylsuccinate synthase, which catalyzes the first step in anaerobic toluene degradation. In cells growing aerobically with benzoate, the addition of toluene resulted in a ~20-fold increased transcription of <i>bssA</i>, encoding for the catalytically active subunit of the enzyme. Under anoxic conditions, <i>bssA</i> mRNA copy numbers were up to 129-fold higher in cells growing with toluene as compared to cells growing with benzoate. Proteomics showed that abundance of benzylsuccinate synthase increased in cells growing anaerobically with toluene. In contrast, peptides of this enzyme were never detected in oxic conditions. These findings show that synthesis of benzylsuccinate synthase was under stringent post-transcriptional control in the presence of oxygen, which is a novel level of regulation for glycyl radical enzymes.https://www.mdpi.com/2076-2607/8/5/681toluenenitratebenzylsuccinate synthase<i>Magnetospirillum</i>rhizosphere |
| spellingShingle | Ingrid Meyer-Cifuentes Sylvie Gruhl Sven-Bastiaan Haange Vanessa Lünsmann Nico Jehmlich Martin von Bergen Hermann J. Heipieper Jochen A. Müller Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier <i>Magnetospirillum</i> sp. Strain 15-1 Microorganisms toluene nitrate benzylsuccinate synthase <i>Magnetospirillum</i> rhizosphere |
| title | Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier <i>Magnetospirillum</i> sp. Strain 15-1 |
| title_full | Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier <i>Magnetospirillum</i> sp. Strain 15-1 |
| title_fullStr | Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier <i>Magnetospirillum</i> sp. Strain 15-1 |
| title_full_unstemmed | Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier <i>Magnetospirillum</i> sp. Strain 15-1 |
| title_short | Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier <i>Magnetospirillum</i> sp. Strain 15-1 |
| title_sort | benzylsuccinate synthase is post transcriptionally regulated in the toluene degrading denitrifier i magnetospirillum i sp strain 15 1 |
| topic | toluene nitrate benzylsuccinate synthase <i>Magnetospirillum</i> rhizosphere |
| url | https://www.mdpi.com/2076-2607/8/5/681 |
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