Serological investigation of Gyrovirus homsa1 infections in chickens in China
Abstract Background Gyrovirus homsa1 (GyH1) (also known as Gyrovirus 3, GyV3) is a non-enveloped, small, single-stranded DNA virus, which was first identified in children with acute diarrhea, and was subsequently detected in marketed chickens, broilers with transmissible viral proventriculitis (TVP)...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03334-0 |
| _version_ | 1811242168316067840 |
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| author | Shicheng Zhang Shiyu Yuan Tianxing Yan Gen Li Xiaojing Hao Defang Zhou Ruiqi Li Yubao Li Ziqiang Cheng |
| author_facet | Shicheng Zhang Shiyu Yuan Tianxing Yan Gen Li Xiaojing Hao Defang Zhou Ruiqi Li Yubao Li Ziqiang Cheng |
| author_sort | Shicheng Zhang |
| collection | DOAJ |
| description | Abstract Background Gyrovirus homsa1 (GyH1) (also known as Gyrovirus 3, GyV3) is a non-enveloped, small, single-stranded DNA virus, which was first identified in children with acute diarrhea, and was subsequently detected in marketed chickens, broilers with transmissible viral proventriculitis (TVP), and mammals. GyH1 is a pathogenic virus in chickens, causing aplastic anemia, immunosuppression, and multisystem damage. However, the seroepidemiology of GyH1 infection in chickens remains unclear. Here, we investigated the seroprevalence of GyH1 in chickens by ELISA to reveal the endemic status of GyH1 in China. Results An indirect ELISA with high sensitivity and specificity was developed for investigation of seroepidemiology of GyH1 in chickens in China. The seropositive rate of GyH1 ranged from 0.6% to 7.7% in thirteen provinces, and ranged from 4.1% to 8.1% in eight species chickens. The seropositive rate of GyH1 in broiler breeders was significantly higher than that of in layers. There was a negative correlation between seropositive rate and age of chickens. The highest and lowest seropositive rate were present in chickens at 30–60 days and over 180 days, respectively. Conclusions The seroepidemiological investigation results demonstrated that natural GyH1 infection is widespread in chickens in China. Different species showed different susceptibility for GyH1. Aged chickens showed obvious age-resistance to GyH1. GyH1 has shown a high risk to the poultry industry and should be highly concerned. |
| first_indexed | 2024-04-12T13:47:49Z |
| format | Article |
| id | doaj.art-d14111f44d05429592264b465805b55d |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-12T13:47:49Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-d14111f44d05429592264b465805b55d2022-12-22T03:30:38ZengBMCBMC Veterinary Research1746-61482022-06-011811810.1186/s12917-022-03334-0Serological investigation of Gyrovirus homsa1 infections in chickens in ChinaShicheng Zhang0Shiyu Yuan1Tianxing Yan2Gen Li3Xiaojing Hao4Defang Zhou5Ruiqi Li6Yubao Li7Ziqiang Cheng8College of Veterinary Medicine, Shandong Agricultural UniversityCollege of Veterinary Medicine, Shandong Agricultural UniversityCollege of Veterinary Medicine, Shandong Agricultural UniversityCollege of Veterinary Medicine, Qingdao Agricultural UniversityQingdao Animal Husbandry Workstation (Qingdao Animal Husbandry and Veterinary Research Institute)College of Veterinary Medicine, Shandong Agricultural UniversityCollege of Veterinary Medicine, Shandong Agricultural UniversityAgricultural Science and Engineering School, Liaocheng UniversityCollege of Veterinary Medicine, Shandong Agricultural UniversityAbstract Background Gyrovirus homsa1 (GyH1) (also known as Gyrovirus 3, GyV3) is a non-enveloped, small, single-stranded DNA virus, which was first identified in children with acute diarrhea, and was subsequently detected in marketed chickens, broilers with transmissible viral proventriculitis (TVP), and mammals. GyH1 is a pathogenic virus in chickens, causing aplastic anemia, immunosuppression, and multisystem damage. However, the seroepidemiology of GyH1 infection in chickens remains unclear. Here, we investigated the seroprevalence of GyH1 in chickens by ELISA to reveal the endemic status of GyH1 in China. Results An indirect ELISA with high sensitivity and specificity was developed for investigation of seroepidemiology of GyH1 in chickens in China. The seropositive rate of GyH1 ranged from 0.6% to 7.7% in thirteen provinces, and ranged from 4.1% to 8.1% in eight species chickens. The seropositive rate of GyH1 in broiler breeders was significantly higher than that of in layers. There was a negative correlation between seropositive rate and age of chickens. The highest and lowest seropositive rate were present in chickens at 30–60 days and over 180 days, respectively. Conclusions The seroepidemiological investigation results demonstrated that natural GyH1 infection is widespread in chickens in China. Different species showed different susceptibility for GyH1. Aged chickens showed obvious age-resistance to GyH1. GyH1 has shown a high risk to the poultry industry and should be highly concerned.https://doi.org/10.1186/s12917-022-03334-0Gyrovirus homsa1SeroepidemiologyIndirect ELISAChicken |
| spellingShingle | Shicheng Zhang Shiyu Yuan Tianxing Yan Gen Li Xiaojing Hao Defang Zhou Ruiqi Li Yubao Li Ziqiang Cheng Serological investigation of Gyrovirus homsa1 infections in chickens in China BMC Veterinary Research Gyrovirus homsa1 Seroepidemiology Indirect ELISA Chicken |
| title | Serological investigation of Gyrovirus homsa1 infections in chickens in China |
| title_full | Serological investigation of Gyrovirus homsa1 infections in chickens in China |
| title_fullStr | Serological investigation of Gyrovirus homsa1 infections in chickens in China |
| title_full_unstemmed | Serological investigation of Gyrovirus homsa1 infections in chickens in China |
| title_short | Serological investigation of Gyrovirus homsa1 infections in chickens in China |
| title_sort | serological investigation of gyrovirus homsa1 infections in chickens in china |
| topic | Gyrovirus homsa1 Seroepidemiology Indirect ELISA Chicken |
| url | https://doi.org/10.1186/s12917-022-03334-0 |
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