Relationship between tumor cell invasiveness and polyploidization.
A number of studies have shown that tumor cells fuse with other tumor and non-tumor cells. In the present study on tumor cell lines derived from glioblastoma, breast cancer, and melanoma, we estimated the frequency of fusion between tumor cells by establishing the fraction of cells with whole tumor-...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3534062?pdf=render |
| _version_ | 1811269531089240064 |
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| author | Javier Mercapide Fabio Anzanello Germana Rappa Aurelio Lorico |
| author_facet | Javier Mercapide Fabio Anzanello Germana Rappa Aurelio Lorico |
| author_sort | Javier Mercapide |
| collection | DOAJ |
| description | A number of studies have shown that tumor cells fuse with other tumor and non-tumor cells. In the present study on tumor cell lines derived from glioblastoma, breast cancer, and melanoma, we estimated the frequency of fusion between tumor cells by establishing the fraction of cells with whole tumor-genome duplication in each cell line. Together with this, the capacity of the tumor cell lines to spread through a basement membrane scaffold was assessed, in order to test the hypothesis that pericellular proteolysis by enzymatic release in the spaces of intercellular contact could account for differences in the fusogenicity of tumor cells. The difference in invasiveness between the cell lines accounted for their specific amount of cells with tumor-genome duplication, which, depending on the cell line analyzed, ranged from 2% to 25% of the total cells. These results support the hypothesis that cell-to-cell invasion eliciting membrane fusion causes polyploidization in tumor cells. |
| first_indexed | 2024-04-12T21:43:43Z |
| format | Article |
| id | doaj.art-d148d550714e4eccb400bfba3d1ad292 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T21:43:43Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d148d550714e4eccb400bfba3d1ad2922022-12-22T03:15:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5336410.1371/journal.pone.0053364Relationship between tumor cell invasiveness and polyploidization.Javier MercapideFabio AnzanelloGermana RappaAurelio LoricoA number of studies have shown that tumor cells fuse with other tumor and non-tumor cells. In the present study on tumor cell lines derived from glioblastoma, breast cancer, and melanoma, we estimated the frequency of fusion between tumor cells by establishing the fraction of cells with whole tumor-genome duplication in each cell line. Together with this, the capacity of the tumor cell lines to spread through a basement membrane scaffold was assessed, in order to test the hypothesis that pericellular proteolysis by enzymatic release in the spaces of intercellular contact could account for differences in the fusogenicity of tumor cells. The difference in invasiveness between the cell lines accounted for their specific amount of cells with tumor-genome duplication, which, depending on the cell line analyzed, ranged from 2% to 25% of the total cells. These results support the hypothesis that cell-to-cell invasion eliciting membrane fusion causes polyploidization in tumor cells.http://europepmc.org/articles/PMC3534062?pdf=render |
| spellingShingle | Javier Mercapide Fabio Anzanello Germana Rappa Aurelio Lorico Relationship between tumor cell invasiveness and polyploidization. PLoS ONE |
| title | Relationship between tumor cell invasiveness and polyploidization. |
| title_full | Relationship between tumor cell invasiveness and polyploidization. |
| title_fullStr | Relationship between tumor cell invasiveness and polyploidization. |
| title_full_unstemmed | Relationship between tumor cell invasiveness and polyploidization. |
| title_short | Relationship between tumor cell invasiveness and polyploidization. |
| title_sort | relationship between tumor cell invasiveness and polyploidization |
| url | http://europepmc.org/articles/PMC3534062?pdf=render |
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