Efficacy of Emilia coccinea aqueous extract on inhibition of α-amylase enzyme activity and insulin resistance in dexamethasone treated-rats
Background: Diabetes mellitus is one of the most common chronic metabolic diseases throughout the world, characterized by hyperglycemia and insulin resistance. The purpose of this study was to evaluate the effects of aqueous extract of Emilia coccinea (AEEC) leaves on dexamethasone-induced insulin r...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Metabolism Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589936822000317 |
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| author | Sl Poualeu Kamani J. Kamgaing Waguia D. Miaffo Ml Nchouwet Cl Demeni Kadji M.T. Wego Kamgaing Rc Douho Djimeli J. Mzoyem Ngnitedem A. Kamanyi Sl Wansi Ngnokam |
| author_facet | Sl Poualeu Kamani J. Kamgaing Waguia D. Miaffo Ml Nchouwet Cl Demeni Kadji M.T. Wego Kamgaing Rc Douho Djimeli J. Mzoyem Ngnitedem A. Kamanyi Sl Wansi Ngnokam |
| author_sort | Sl Poualeu Kamani |
| collection | DOAJ |
| description | Background: Diabetes mellitus is one of the most common chronic metabolic diseases throughout the world, characterized by hyperglycemia and insulin resistance. The purpose of this study was to evaluate the effects of aqueous extract of Emilia coccinea (AEEC) leaves on dexamethasone-induced insulin resistance in rats and on in vitro α-amylase enzyme activity. Methods: Insulin resistance was induced by intraperitoneal injection of dexamethasone (1 mg/kg) for 8 days in rats. The animals were concomitant received extracts at doses of 107.5; 215; 430 mg/kg for this period. At the end of the treatment, blood glucose level, lipid profile, transaminases, triglyceride glucose (TyG) index, body mass and relative organ weight were evaluated. Results: The results showed that AEEC inhibits α-amylase enzyme with an IC50 of 34.10 μg/ml in vitro. AEEC significantly reduced blood glucose level, triglycerides, TyG index, total and LDL cholesterol, liver weight and increased HDL cholesterol. Moreover, it reduced ALAT and ASAT activity. These parameters were strongly modify by dexamethasone. Conclusion: AEEC plays antidiabetic roles by ameliorating insulin resistance and reducing postprandial blood glucose level through α-amylase enzyme inhibition. |
| first_indexed | 2024-04-12T20:57:25Z |
| format | Article |
| id | doaj.art-d14bc2915e02465da5e3d005f232aded |
| institution | Directory Open Access Journal |
| issn | 2589-9368 |
| language | English |
| last_indexed | 2024-04-12T20:57:25Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolism Open |
| spelling | doaj.art-d14bc2915e02465da5e3d005f232aded2022-12-22T03:16:56ZengElsevierMetabolism Open2589-93682022-09-0115100193Efficacy of Emilia coccinea aqueous extract on inhibition of α-amylase enzyme activity and insulin resistance in dexamethasone treated-ratsSl Poualeu Kamani0J. Kamgaing Waguia1D. Miaffo2Ml Nchouwet3Cl Demeni Kadji4M.T. Wego Kamgaing5Rc Douho Djimeli6J. Mzoyem Ngnitedem7A. Kamanyi8Sl Wansi Ngnokam9Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, CameroonDepartment of Life and Earth Sciences, Higher Teachers' Training College, University of Maroua, CameroonLaboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Sciences, University of Dschang, Cameroon; Corresponding author.Background: Diabetes mellitus is one of the most common chronic metabolic diseases throughout the world, characterized by hyperglycemia and insulin resistance. The purpose of this study was to evaluate the effects of aqueous extract of Emilia coccinea (AEEC) leaves on dexamethasone-induced insulin resistance in rats and on in vitro α-amylase enzyme activity. Methods: Insulin resistance was induced by intraperitoneal injection of dexamethasone (1 mg/kg) for 8 days in rats. The animals were concomitant received extracts at doses of 107.5; 215; 430 mg/kg for this period. At the end of the treatment, blood glucose level, lipid profile, transaminases, triglyceride glucose (TyG) index, body mass and relative organ weight were evaluated. Results: The results showed that AEEC inhibits α-amylase enzyme with an IC50 of 34.10 μg/ml in vitro. AEEC significantly reduced blood glucose level, triglycerides, TyG index, total and LDL cholesterol, liver weight and increased HDL cholesterol. Moreover, it reduced ALAT and ASAT activity. These parameters were strongly modify by dexamethasone. Conclusion: AEEC plays antidiabetic roles by ameliorating insulin resistance and reducing postprandial blood glucose level through α-amylase enzyme inhibition.http://www.sciencedirect.com/science/article/pii/S2589936822000317Emilia coccineaα-amylase enzymeDexamethasoneInsulin resistanceRats |
| spellingShingle | Sl Poualeu Kamani J. Kamgaing Waguia D. Miaffo Ml Nchouwet Cl Demeni Kadji M.T. Wego Kamgaing Rc Douho Djimeli J. Mzoyem Ngnitedem A. Kamanyi Sl Wansi Ngnokam Efficacy of Emilia coccinea aqueous extract on inhibition of α-amylase enzyme activity and insulin resistance in dexamethasone treated-rats Metabolism Open Emilia coccinea α-amylase enzyme Dexamethasone Insulin resistance Rats |
| title | Efficacy of Emilia coccinea aqueous extract on inhibition of α-amylase enzyme activity and insulin resistance in dexamethasone treated-rats |
| title_full | Efficacy of Emilia coccinea aqueous extract on inhibition of α-amylase enzyme activity and insulin resistance in dexamethasone treated-rats |
| title_fullStr | Efficacy of Emilia coccinea aqueous extract on inhibition of α-amylase enzyme activity and insulin resistance in dexamethasone treated-rats |
| title_full_unstemmed | Efficacy of Emilia coccinea aqueous extract on inhibition of α-amylase enzyme activity and insulin resistance in dexamethasone treated-rats |
| title_short | Efficacy of Emilia coccinea aqueous extract on inhibition of α-amylase enzyme activity and insulin resistance in dexamethasone treated-rats |
| title_sort | efficacy of emilia coccinea aqueous extract on inhibition of α amylase enzyme activity and insulin resistance in dexamethasone treated rats |
| topic | Emilia coccinea α-amylase enzyme Dexamethasone Insulin resistance Rats |
| url | http://www.sciencedirect.com/science/article/pii/S2589936822000317 |
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