Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensis
In order to investigate the influence of environmental conditions on biofilm formation ability of food spoilage strain Pseudomonas lundensis (PL), the biofilm amount was measured by microplate crystal violet method under different nutritional conditions, inoculation concentration, pH, NaCl and Mg2+...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-02-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030335 |
| _version_ | 1811169260003655680 |
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| author | Yongji LIU Genmei LIU Guoling LIU Linzhi KANG |
| author_facet | Yongji LIU Genmei LIU Guoling LIU Linzhi KANG |
| author_sort | Yongji LIU |
| collection | DOAJ |
| description | In order to investigate the influence of environmental conditions on biofilm formation ability of food spoilage strain Pseudomonas lundensis (PL), the biofilm amount was measured by microplate crystal violet method under different nutritional conditions, inoculation concentration, pH, NaCl and Mg2+ concentration conditions. The adhesion and structural characteristics of PL on stainless steel surface were investigated by laser scanning confocal microscope. The results showed that nutritional stress formed by dilute tryptone soy broth reduced the amount of PL biofilm formation, and the nutrient stress caused by 50-fold dilution of TSB medium reduced the amount of biofilm from 1.75±0.35 to 0.24±0.17. There was no significant difference in biofilm formation when PL inoculation amount decreased from 1.3×107 CFU/ mL to 2.5×104 CFU/mL (P>0.05). The highest biofilm production was observed at pH 8.0. The biofilm formation was significantly inhibited respectively when the addition of glucose exceeded 1.25%, NaCl exceeded 4% and Mg2+ exceeded 0.5%. The observation results of laser scanning confocal microscopy showed that the PL had the ability to form typical biofilm on stainless steel surface at a certain concentration. The ability of PL biofilm formation was influenced by environmental factors such as nutrient stress, glucose concentration, pH, NaCl and Mg2+ concentration. |
| first_indexed | 2024-04-10T16:39:25Z |
| format | Article |
| id | doaj.art-d14c7ee93c6f4fd98418d66e97da9ba3 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-10T16:39:25Z |
| publishDate | 2023-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-d14c7ee93c6f4fd98418d66e97da9ba32023-02-08T08:00:16ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-02-0144412212810.13386/j.issn1002-0306.20220303352022030335-4Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensisYongji LIU0Genmei LIU1Guoling LIU2Linzhi KANG3Henry Fok School of Food Science and Engineering, Shaoguan University, Shaoguan 512005, ChinaHenry Fok School of Food Science and Engineering, Shaoguan University, Shaoguan 512005, ChinaHenry Fok School of Food Science and Engineering, Shaoguan University, Shaoguan 512005, ChinaHenry Fok School of Food Science and Engineering, Shaoguan University, Shaoguan 512005, ChinaIn order to investigate the influence of environmental conditions on biofilm formation ability of food spoilage strain Pseudomonas lundensis (PL), the biofilm amount was measured by microplate crystal violet method under different nutritional conditions, inoculation concentration, pH, NaCl and Mg2+ concentration conditions. The adhesion and structural characteristics of PL on stainless steel surface were investigated by laser scanning confocal microscope. The results showed that nutritional stress formed by dilute tryptone soy broth reduced the amount of PL biofilm formation, and the nutrient stress caused by 50-fold dilution of TSB medium reduced the amount of biofilm from 1.75±0.35 to 0.24±0.17. There was no significant difference in biofilm formation when PL inoculation amount decreased from 1.3×107 CFU/ mL to 2.5×104 CFU/mL (P>0.05). The highest biofilm production was observed at pH 8.0. The biofilm formation was significantly inhibited respectively when the addition of glucose exceeded 1.25%, NaCl exceeded 4% and Mg2+ exceeded 0.5%. The observation results of laser scanning confocal microscopy showed that the PL had the ability to form typical biofilm on stainless steel surface at a certain concentration. The ability of PL biofilm formation was influenced by environmental factors such as nutrient stress, glucose concentration, pH, NaCl and Mg2+ concentration.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030335pseudomonas lundensisbiofilmenvironmental conditionsspoilage bacteria |
| spellingShingle | Yongji LIU Genmei LIU Guoling LIU Linzhi KANG Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensis Shipin gongye ke-ji pseudomonas lundensis biofilm environmental conditions spoilage bacteria |
| title | Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensis |
| title_full | Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensis |
| title_fullStr | Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensis |
| title_full_unstemmed | Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensis |
| title_short | Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensis |
| title_sort | impact of environmental conditions on the biofilm formation of pseudomonas lundensis |
| topic | pseudomonas lundensis biofilm environmental conditions spoilage bacteria |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030335 |
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