Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic rats
In the present study, the effects of ethanolic root extract of Codariocalyx motorius (CMRt) on blood glucose, insulin, and carbohydrate metabolic enzymes were studied in experimental diabetic rats. Streptozotocin-induced diabetic rats received CMRt orally at the doses of 100 and 200 mg/kg bw daily f...
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| Format: | Article |
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Elsevier
2014-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614002564 |
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| author | C. Uma N. Suganya P. Vanitha E. Bhakkiyalakshmi S. Suriyanarayanan K.M. Maria John S. Sivasubramanian P. Gunasekaran K.M. Ramkumar |
| author_facet | C. Uma N. Suganya P. Vanitha E. Bhakkiyalakshmi S. Suriyanarayanan K.M. Maria John S. Sivasubramanian P. Gunasekaran K.M. Ramkumar |
| author_sort | C. Uma |
| collection | DOAJ |
| description | In the present study, the effects of ethanolic root extract of Codariocalyx motorius (CMRt) on blood glucose, insulin, and carbohydrate metabolic enzymes were studied in experimental diabetic rats. Streptozotocin-induced diabetic rats received CMRt orally at the doses of 100 and 200 mg/kg bw daily for 30 days. CMRt treatment showed decrease in blood glucose and glycosylated hemoglobin with improved serum insulin levels. We observed increase in carbohydrate metabolizing enzymes including hexokinase, glucose-6-phosphate-dehydrogenase and glycogen content in liver of CMRt-treated rats with reduction in the levels of glucose-6-phosphatase and fructose-1,6-bisphosphatase. Further, immunohistochemical staining of insulin showed preservation of pancreatic β-cells in CMRt-treated diabetic rats. RP-HPLC analysis indicated the presence of chlorogenic acid, myricetin, quercetin and gallic acid. Hence, this study clearly demonstrates the antidiabetic effect of CMRt by normalizing the key enzymes of carbohydrate metabolism and restoring the functions of pancreas thus proving its traditional value. |
| first_indexed | 2024-12-17T12:30:39Z |
| format | Article |
| id | doaj.art-d14d8d7208ae43759f904c22e836a1b9 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T12:30:39Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-d14d8d7208ae43759f904c22e836a1b92022-12-21T21:48:35ZengElsevierJournal of Functional Foods1756-46462014-11-0111517527Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic ratsC. Uma0N. Suganya1P. Vanitha2E. Bhakkiyalakshmi3S. Suriyanarayanan4K.M. Maria John5S. Sivasubramanian6P. Gunasekaran7K.M. Ramkumar8Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur-603 203, Tamilnadu, IndiaDepartment of Biotechnology, School of Bioengineering, SRM University, Kattankulathur-603 203, Tamilnadu, IndiaDepartment of Biotechnology, School of Bioengineering, SRM University, Kattankulathur-603 203, Tamilnadu, IndiaDepartment of Biotechnology, School of Bioengineering, SRM University, Kattankulathur-603 203, Tamilnadu, IndiaDepartment of Water and Health, JSS University, Mysore-570 015, Karnataka, IndiaDepartment of Bioresources and Food Technology, Konkuk University, Gwangjin-gu, Seoul 143-701, KoreaThe King Institute of Preventive Medicine and Research, Guindy, Chennai-600 032, Tamilnadu, IndiaThe King Institute of Preventive Medicine and Research, Guindy, Chennai-600 032, Tamilnadu, IndiaSRM Research Institute, SRM University, Kattankulathur-603 203, Tamilnadu, India; Corresponding author. Tel.: +91-44-27417915; fax: +91-44-2745 2343.In the present study, the effects of ethanolic root extract of Codariocalyx motorius (CMRt) on blood glucose, insulin, and carbohydrate metabolic enzymes were studied in experimental diabetic rats. Streptozotocin-induced diabetic rats received CMRt orally at the doses of 100 and 200 mg/kg bw daily for 30 days. CMRt treatment showed decrease in blood glucose and glycosylated hemoglobin with improved serum insulin levels. We observed increase in carbohydrate metabolizing enzymes including hexokinase, glucose-6-phosphate-dehydrogenase and glycogen content in liver of CMRt-treated rats with reduction in the levels of glucose-6-phosphatase and fructose-1,6-bisphosphatase. Further, immunohistochemical staining of insulin showed preservation of pancreatic β-cells in CMRt-treated diabetic rats. RP-HPLC analysis indicated the presence of chlorogenic acid, myricetin, quercetin and gallic acid. Hence, this study clearly demonstrates the antidiabetic effect of CMRt by normalizing the key enzymes of carbohydrate metabolism and restoring the functions of pancreas thus proving its traditional value.http://www.sciencedirect.com/science/article/pii/S1756464614002564Codariocalyx motoriusAntidiabetic activityStreptozotocinCarbohydrate enzymesMedicinal plants |
| spellingShingle | C. Uma N. Suganya P. Vanitha E. Bhakkiyalakshmi S. Suriyanarayanan K.M. Maria John S. Sivasubramanian P. Gunasekaran K.M. Ramkumar Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic rats Journal of Functional Foods Codariocalyx motorius Antidiabetic activity Streptozotocin Carbohydrate enzymes Medicinal plants |
| title | Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic rats |
| title_full | Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic rats |
| title_fullStr | Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic rats |
| title_full_unstemmed | Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic rats |
| title_short | Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic rats |
| title_sort | antihyperglycemic effect of codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin induced diabetic rats |
| topic | Codariocalyx motorius Antidiabetic activity Streptozotocin Carbohydrate enzymes Medicinal plants |
| url | http://www.sciencedirect.com/science/article/pii/S1756464614002564 |
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