Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China
Abstract Background Anaplasma spp. are tick-transmitted bacteria that infect a wide variety of wild and domestic animals. These pathogens exhibit a high degree of biological diversity, broad geographical distribution, and represent a serious threat to veterinary and public health worldwide. Results...
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| Format: | Article |
| Language: | English |
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BMC
2016-11-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-016-1886-6 |
| _version_ | 1828334201370312704 |
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| author | Jifei Yang Zhijie Liu Qingli Niu Junlong Liu Rong Han Guangyuan Liu Yaoxu Shi Jianxun Luo Hong Yin |
| author_facet | Jifei Yang Zhijie Liu Qingli Niu Junlong Liu Rong Han Guangyuan Liu Yaoxu Shi Jianxun Luo Hong Yin |
| author_sort | Jifei Yang |
| collection | DOAJ |
| description | Abstract Background Anaplasma spp. are tick-transmitted bacteria that infect a wide variety of wild and domestic animals. These pathogens exhibit a high degree of biological diversity, broad geographical distribution, and represent a serious threat to veterinary and public health worldwide. Results A novel Anaplasma species was identified in Haemaphysalis qinghaiensis (Ixodidae) in northwestern China and was molecularly characterized by comparison of 16S rRNA, gltA, and groEL gene sequences. Of the 414 samples tested, 24 (5.8%) were positive for this Anaplasma species. On the basis of the 16S rRNA gene, this organism has been found to be closely related to and exhibit the highest sequence similarity with A. capra (99.8–99.9%) that was identified in goats and humans in northern China, but was distinct from other known Anaplasma species. Sequence analysis of the gltA and groEL genes revealed that this Anaplasma species was distinct from A. capra considering the lower sequence identity (88.6–88.7% for gltA and 90.6–91.0% for groEL) and a divergent phylogenetic position. Therefore, we described this Anaplasma species as A. capra-like bacteria. Conclusions The present study reports a potential novel Anaplasma species closely related to A. capra in H. qinghaiensis in northwestern China. The zoonotic potential of A. capra-like bacteria needs to be further determined. |
| first_indexed | 2024-04-13T21:30:04Z |
| format | Article |
| id | doaj.art-d151676f8e67499497ac744ceddb966d |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-13T21:30:04Z |
| publishDate | 2016-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-d151676f8e67499497ac744ceddb966d2022-12-22T02:29:12ZengBMCParasites & Vectors1756-33052016-11-01911510.1186/s13071-016-1886-6Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern ChinaJifei Yang0Zhijie Liu1Qingli Niu2Junlong Liu3Rong Han4Guangyuan Liu5Yaoxu Shi6Jianxun Luo7Hong Yin8State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ScienceState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ScienceState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ScienceState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ScienceState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ScienceState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ScienceChina Agricultural Veterinary Biological Sciences and Technology Co., LtdState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ScienceState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ScienceAbstract Background Anaplasma spp. are tick-transmitted bacteria that infect a wide variety of wild and domestic animals. These pathogens exhibit a high degree of biological diversity, broad geographical distribution, and represent a serious threat to veterinary and public health worldwide. Results A novel Anaplasma species was identified in Haemaphysalis qinghaiensis (Ixodidae) in northwestern China and was molecularly characterized by comparison of 16S rRNA, gltA, and groEL gene sequences. Of the 414 samples tested, 24 (5.8%) were positive for this Anaplasma species. On the basis of the 16S rRNA gene, this organism has been found to be closely related to and exhibit the highest sequence similarity with A. capra (99.8–99.9%) that was identified in goats and humans in northern China, but was distinct from other known Anaplasma species. Sequence analysis of the gltA and groEL genes revealed that this Anaplasma species was distinct from A. capra considering the lower sequence identity (88.6–88.7% for gltA and 90.6–91.0% for groEL) and a divergent phylogenetic position. Therefore, we described this Anaplasma species as A. capra-like bacteria. Conclusions The present study reports a potential novel Anaplasma species closely related to A. capra in H. qinghaiensis in northwestern China. The zoonotic potential of A. capra-like bacteria needs to be further determined.http://link.springer.com/article/10.1186/s13071-016-1886-6Anaplasma16S rRNA genegltA genegroEL geneHaemaphysalis qinghaiensisA. capra-like bacteria |
| spellingShingle | Jifei Yang Zhijie Liu Qingli Niu Junlong Liu Rong Han Guangyuan Liu Yaoxu Shi Jianxun Luo Hong Yin Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China Parasites & Vectors Anaplasma 16S rRNA gene gltA gene groEL gene Haemaphysalis qinghaiensis A. capra-like bacteria |
| title | Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China |
| title_full | Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China |
| title_fullStr | Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China |
| title_full_unstemmed | Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China |
| title_short | Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China |
| title_sort | molecular survey and characterization of a novel anaplasma species closely related to anaplasma capra in ticks northwestern china |
| topic | Anaplasma 16S rRNA gene gltA gene groEL gene Haemaphysalis qinghaiensis A. capra-like bacteria |
| url | http://link.springer.com/article/10.1186/s13071-016-1886-6 |
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