3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications
In this work, we developed and analyzed a biphasic calcium phosphate (BCP) bioceramic for bone regeneration using stereolithography (SLA). The SLA method is a promising additive manufacturing (AM) technique capable of creating BCp parts with high accuracy and efficiency. However, the ceramic suspens...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Annals of 3D Printed Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666964124000079 |
| _version_ | 1797317082549846016 |
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| author | K. Prem Ananth Naidu Dhanpal Jayram Kandasamy Muthusamy |
| author_facet | K. Prem Ananth Naidu Dhanpal Jayram Kandasamy Muthusamy |
| author_sort | K. Prem Ananth |
| collection | DOAJ |
| description | In this work, we developed and analyzed a biphasic calcium phosphate (BCP) bioceramic for bone regeneration using stereolithography (SLA). The SLA method is a promising additive manufacturing (AM) technique capable of creating BCp parts with high accuracy and efficiency. However, the ceramic suspension used in SLA exhibits significantly higher viscosity and is not environmentally friendly. Therefore, adequate preparation of a suspension with low viscosity and high solid loading is essential. In this paper, we optimized the effects of surfactant doses and solid loading on the BCp slurry, and initially examined the process parameters of photocuring, debinding, and sintering. The utilization of 9 wt % Disperbyk (BYK) with a 40 vol % loading of BCp bioceramics exhibited a reasonably low viscosity of 8.9 mPa·s at a shear level of 46.5 s−1. Functional and structural analyses confirmed that BCp was retained after photocuring and subsequent treatment, which were incorporated into the BYK dispersion. The 3D printed objects with different sintered temperatures, specifically at 1100 °C, 1200 °C, and 1300 °C, were further optimized. Additionally, the surface roughness, porosity, and mechanical properties of BCp green parts were systematically investigated. Most importantly, in vitro analysis of cell attachment, differentiation, and red alizarin analysis could support the application of bone regeneration. |
| first_indexed | 2024-03-08T03:29:43Z |
| format | Article |
| id | doaj.art-d152474bd45c4edf95190911378c34c1 |
| institution | Directory Open Access Journal |
| issn | 2666-9641 |
| language | English |
| last_indexed | 2024-03-08T03:29:43Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of 3D Printed Medicine |
| spelling | doaj.art-d152474bd45c4edf95190911378c34c12024-02-11T05:12:36ZengElsevierAnnals of 3D Printed Medicine2666-96412024-05-01141001483D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applicationsK. Prem Ananth0Naidu Dhanpal Jayram1Kandasamy Muthusamy2Department of Physics, Kalasalingam Academy of Research and Education, Tamil Nadu, Krishnankovil 626126, India; Corresponding author.Department of Physics, Kalasalingam Academy of Research and Education, Tamil Nadu, Krishnankovil 626126, IndiaInstitute of Power Engineering, Universiti Tenaga Nasional, Kajang 43000, MalaysiaIn this work, we developed and analyzed a biphasic calcium phosphate (BCP) bioceramic for bone regeneration using stereolithography (SLA). The SLA method is a promising additive manufacturing (AM) technique capable of creating BCp parts with high accuracy and efficiency. However, the ceramic suspension used in SLA exhibits significantly higher viscosity and is not environmentally friendly. Therefore, adequate preparation of a suspension with low viscosity and high solid loading is essential. In this paper, we optimized the effects of surfactant doses and solid loading on the BCp slurry, and initially examined the process parameters of photocuring, debinding, and sintering. The utilization of 9 wt % Disperbyk (BYK) with a 40 vol % loading of BCp bioceramics exhibited a reasonably low viscosity of 8.9 mPa·s at a shear level of 46.5 s−1. Functional and structural analyses confirmed that BCp was retained after photocuring and subsequent treatment, which were incorporated into the BYK dispersion. The 3D printed objects with different sintered temperatures, specifically at 1100 °C, 1200 °C, and 1300 °C, were further optimized. Additionally, the surface roughness, porosity, and mechanical properties of BCp green parts were systematically investigated. Most importantly, in vitro analysis of cell attachment, differentiation, and red alizarin analysis could support the application of bone regeneration.http://www.sciencedirect.com/science/article/pii/S2666964124000079Biphasic calcium phosphateStereolithographyPhotopolymerization3D printingTissue engineering |
| spellingShingle | K. Prem Ananth Naidu Dhanpal Jayram Kandasamy Muthusamy 3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications Annals of 3D Printed Medicine Biphasic calcium phosphate Stereolithography Photopolymerization 3D printing Tissue engineering |
| title | 3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_full | 3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_fullStr | 3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_full_unstemmed | 3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_short | 3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_sort | 3d printed biphasic calcium phosphate scaffold to augment cytocompatibility evaluation for load bearing implant applications |
| topic | Biphasic calcium phosphate Stereolithography Photopolymerization 3D printing Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666964124000079 |
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