Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence
The clinical and preclinical research of ischemic strokes (IS) is becoming increasingly comprehensive, especially with the emerging evidence of complex thrombotic and inflammatory interactions. Within these, the blood brain barrier (BBB) plays an important role in regulating the cellular interaction...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/4/1/23 |
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| author | Maximilian Franke Michael Bieber Guido Stoll Michael Klaus Schuhmann |
| author_facet | Maximilian Franke Michael Bieber Guido Stoll Michael Klaus Schuhmann |
| author_sort | Maximilian Franke |
| collection | DOAJ |
| description | The clinical and preclinical research of ischemic strokes (IS) is becoming increasingly comprehensive, especially with the emerging evidence of complex thrombotic and inflammatory interactions. Within these, the blood brain barrier (BBB) plays an important role in regulating the cellular interactions at the vascular interface and is therefore the object of many IS-related questions. Consequently, valid, economic and responsible methods to define BBB integrity are necessary. Therefore, we compared the three ex-vivo setups albumin Western blot (WB), IgG WB and albumin intensity measurement (AIM) with regard to validity as well as temporal and economic efficacy. While the informative value of the three methods correlated significantly, the efficacy of the IgG WB dominated. |
| first_indexed | 2024-03-10T13:00:23Z |
| format | Article |
| id | doaj.art-d1569554b28643b99026d1e0725dbc5b |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-10T13:00:23Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-d1569554b28643b99026d1e0725dbc5b2023-11-21T11:36:28ZengMDPI AGMethods and Protocols2409-92792021-03-01412310.3390/mps4010023Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin ImmunofluorescenceMaximilian Franke0Michael Bieber1Guido Stoll2Michael Klaus Schuhmann3Department of Neurology, University Hospital Wuerzburg, Josef-Schneider-Str. 11, 97080 Würzburg, GermanyDepartment of Neurology, University Hospital Wuerzburg, Josef-Schneider-Str. 11, 97080 Würzburg, GermanyDepartment of Neurology, University Hospital Wuerzburg, Josef-Schneider-Str. 11, 97080 Würzburg, GermanyDepartment of Neurology, University Hospital Wuerzburg, Josef-Schneider-Str. 11, 97080 Würzburg, GermanyThe clinical and preclinical research of ischemic strokes (IS) is becoming increasingly comprehensive, especially with the emerging evidence of complex thrombotic and inflammatory interactions. Within these, the blood brain barrier (BBB) plays an important role in regulating the cellular interactions at the vascular interface and is therefore the object of many IS-related questions. Consequently, valid, economic and responsible methods to define BBB integrity are necessary. Therefore, we compared the three ex-vivo setups albumin Western blot (WB), IgG WB and albumin intensity measurement (AIM) with regard to validity as well as temporal and economic efficacy. While the informative value of the three methods correlated significantly, the efficacy of the IgG WB dominated.https://www.mdpi.com/2409-9279/4/1/23IgGalbuminimmunohistochemistryWestern blotstroketMCAO |
| spellingShingle | Maximilian Franke Michael Bieber Guido Stoll Michael Klaus Schuhmann Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence Methods and Protocols IgG albumin immunohistochemistry Western blot stroke tMCAO |
| title | Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence |
| title_full | Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence |
| title_fullStr | Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence |
| title_full_unstemmed | Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence |
| title_short | Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence |
| title_sort | validity and efficacy of methods to define blood brain barrier integrity in experimental ischemic strokes a comparison of albumin western blot igg western blot and albumin immunofluorescence |
| topic | IgG albumin immunohistochemistry Western blot stroke tMCAO |
| url | https://www.mdpi.com/2409-9279/4/1/23 |
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