Production of new rhamnolipids Rha C16-C16 by Burkholderia sp. through biodegradation of diesel and biodiesel
Burkholderia sp. C3 was previously isolated from polycyclic aromatic hydrocarbons (PAH) contaminated soil; it was evaluated for the ability to utilize a range of diesel and biodiesel as sole carbon sources. Burkholderia sp. C3 could degrade 99% of diesel and biodiesel supplemented at 2% (v/v) in a b...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-12-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314853518300386 |
| _version_ | 1818765237053030400 |
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| author | A.B. Abeer Mohammed Ahmed A. Tayel Nihal M. Elguindy |
| author_facet | A.B. Abeer Mohammed Ahmed A. Tayel Nihal M. Elguindy |
| author_sort | A.B. Abeer Mohammed |
| collection | DOAJ |
| description | Burkholderia sp. C3 was previously isolated from polycyclic aromatic hydrocarbons (PAH) contaminated soil; it was evaluated for the ability to utilize a range of diesel and biodiesel as sole carbon sources. Burkholderia sp. C3 could degrade 99% of diesel and biodiesel supplemented at 2% (v/v) in a basal mineral medium within 3 days of incubation at 30 °C. The produced rhamnolipids (RL), from diesel degrading bacteria, were characterized by Orcinol test and Mass spectrometry (MS). The MS confirmed that RLs structure compounds had a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic and di-rhamno-di-lipidic congeners. Burkholderia sp. produced a mixture of rhamnolipid homologues, as identified by mass spectrometry, while the composition of rhamnolipids depended upon the carbon substrates. The homologues rhamnolipid Rha-Rha-C10-C10, Rha-C10-C10 and Rha-Rha-C12-C12 were the predominant structures. Rha-C12-C14, Rha-C10-C12, Rha-Rha-C10-C12, and Rha-Rha-C12-C14 were detected as minor components. The efficient employment of Burkholderia sp., for the bioremediation of diesel contamination and production of RLs from it, could be recommended from this study. Keywords: Bioremediation, Biosurfactant, Mass analysis, Oil Spills |
| first_indexed | 2024-12-18T08:14:54Z |
| format | Article |
| id | doaj.art-d157d92d1b714b8494bffd1470f045a0 |
| institution | Directory Open Access Journal |
| issn | 2314-8535 |
| language | English |
| last_indexed | 2024-12-18T08:14:54Z |
| publishDate | 2018-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-d157d92d1b714b8494bffd1470f045a02022-12-21T21:14:50ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85352018-12-0174492498Production of new rhamnolipids Rha C16-C16 by Burkholderia sp. through biodegradation of diesel and biodieselA.B. Abeer Mohammed0Ahmed A. Tayel1Nihal M. Elguindy2Genetic Engineering and Biotechnology Research Institute, University of Sadat City, EgyptFaculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Egypt; Corresponding author at: Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, El-Geish St., 33516 Kafrelsheikh City, Egypt.Department of Biochemistry, Faculty of Science, Alexandria University, EgyptBurkholderia sp. C3 was previously isolated from polycyclic aromatic hydrocarbons (PAH) contaminated soil; it was evaluated for the ability to utilize a range of diesel and biodiesel as sole carbon sources. Burkholderia sp. C3 could degrade 99% of diesel and biodiesel supplemented at 2% (v/v) in a basal mineral medium within 3 days of incubation at 30 °C. The produced rhamnolipids (RL), from diesel degrading bacteria, were characterized by Orcinol test and Mass spectrometry (MS). The MS confirmed that RLs structure compounds had a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic and di-rhamno-di-lipidic congeners. Burkholderia sp. produced a mixture of rhamnolipid homologues, as identified by mass spectrometry, while the composition of rhamnolipids depended upon the carbon substrates. The homologues rhamnolipid Rha-Rha-C10-C10, Rha-C10-C10 and Rha-Rha-C12-C12 were the predominant structures. Rha-C12-C14, Rha-C10-C12, Rha-Rha-C10-C12, and Rha-Rha-C12-C14 were detected as minor components. The efficient employment of Burkholderia sp., for the bioremediation of diesel contamination and production of RLs from it, could be recommended from this study. Keywords: Bioremediation, Biosurfactant, Mass analysis, Oil Spillshttp://www.sciencedirect.com/science/article/pii/S2314853518300386 |
| spellingShingle | A.B. Abeer Mohammed Ahmed A. Tayel Nihal M. Elguindy Production of new rhamnolipids Rha C16-C16 by Burkholderia sp. through biodegradation of diesel and biodiesel Beni-Suef University Journal of Basic and Applied Sciences |
| title | Production of new rhamnolipids Rha C16-C16 by Burkholderia sp. through biodegradation of diesel and biodiesel |
| title_full | Production of new rhamnolipids Rha C16-C16 by Burkholderia sp. through biodegradation of diesel and biodiesel |
| title_fullStr | Production of new rhamnolipids Rha C16-C16 by Burkholderia sp. through biodegradation of diesel and biodiesel |
| title_full_unstemmed | Production of new rhamnolipids Rha C16-C16 by Burkholderia sp. through biodegradation of diesel and biodiesel |
| title_short | Production of new rhamnolipids Rha C16-C16 by Burkholderia sp. through biodegradation of diesel and biodiesel |
| title_sort | production of new rhamnolipids rha c16 c16 by burkholderia sp through biodegradation of diesel and biodiesel |
| url | http://www.sciencedirect.com/science/article/pii/S2314853518300386 |
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