In Vitro Comparison of Antifungal Activity between Epigallocatechin Gallate EGCG) and Nystatin on Candida Sp. Stored Isolates in HIV/AIDS Patients with Oral Candidiasis
Background: Oral candidiasis is an oral infection caused by Candida albicans. It is one of the most common opportunistic infections found in patients with Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS). Due to growing resistance and side effects to common antifungal dr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | Indonesian |
| Published: |
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga
2021-07-01
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| Series: | Berkala Ilmu Kesehatan Kulit dan Kelamin (Periodical of Dermatology and Venerology) |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/BIKK/article/view/21775 |
| _version_ | 1797506058359406592 |
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| author | Yusuf Wibisono Afif Nurul Hidayati Sawitri Sawitri Iskandar Zulkarnain Evy Ervianti Rahmadewi Rahmadewi Esti Hendradi Pepy Dwi Endraswari Dwi Murtiastutik |
| author_facet | Yusuf Wibisono Afif Nurul Hidayati Sawitri Sawitri Iskandar Zulkarnain Evy Ervianti Rahmadewi Rahmadewi Esti Hendradi Pepy Dwi Endraswari Dwi Murtiastutik |
| author_sort | Yusuf Wibisono |
| collection | DOAJ |
| description | Background: Oral candidiasis is an oral infection caused by Candida albicans. It is one of the most common opportunistic infections found in patients with Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS). Due to growing resistance and side effects to common antifungal drugs in recent years, there have been many studies on natural
substances as antifungal agents. In this study, Epigallocatechin gallate (EGCG) were examined for in vitro antifungal activity against Candida strains. Purpose: To compare the in vitro antifungal activity of EGCG and nystatin towards Candida sp. isolate in HIV/AIDS patients with oral candidiasis. Methods: This research was an experimental laboratory study conducted at the Dr. Soetomo General Academic Hospital, Surabaya. Forty isolates of Candida albicans and Candida non-albicans were included. The isolates were tested for antifungal activity using the well diffusion and microdilution method for nystatin 1000 IU and EGCG 1.25%. Result: Diffusion test results showed a greater inhibition zone for nystatin against all analyzed Candida strains with an average diameter for Candida albicans formed by EGCG of 2.15 mm and 7.4 mm for Candida non-albicans. Meanwhile, based on the microdilution test, EGCG was better than nystatin towards all analyzed Candida strains. This study showed EGCG Minimum Fungicidal Concentration (MFC) as high as 50% (p < 0.05). Conclusion: Nystatin antifungal activity was better than EGCG supported by a greater inhibition zone in the well diffusion method. However, the antifungal activity of EGCG was better than nystatin based on microdilution methods. |
| first_indexed | 2024-03-10T04:27:11Z |
| format | Article |
| id | doaj.art-d157fc39e94a445ab66737055411b6ed |
| institution | Directory Open Access Journal |
| issn | 1978-4279 2549-4082 |
| language | Indonesian |
| last_indexed | 2024-03-10T04:27:11Z |
| publishDate | 2021-07-01 |
| publisher | Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga |
| record_format | Article |
| series | Berkala Ilmu Kesehatan Kulit dan Kelamin (Periodical of Dermatology and Venerology) |
| spelling | doaj.art-d157fc39e94a445ab66737055411b6ed2023-11-23T06:33:57ZindDepartment of Dermatology and Venereology, Faculty of Medicine, Universitas AirlanggaBerkala Ilmu Kesehatan Kulit dan Kelamin (Periodical of Dermatology and Venerology)1978-42792549-40822021-07-0133210310910.20473/bikk.V33.2.2021.103-10917818In Vitro Comparison of Antifungal Activity between Epigallocatechin Gallate EGCG) and Nystatin on Candida Sp. Stored Isolates in HIV/AIDS Patients with Oral CandidiasisYusuf Wibisono0Afif Nurul Hidayati1Sawitri Sawitri2Iskandar Zulkarnain3Evy Ervianti4Rahmadewi Rahmadewi5Esti Hendradi6Pepy Dwi Endraswari7Dwi Murtiastutik8Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, SurabayaDepartment of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, SurabayaDepartment of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, SurabayaDepartment of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, SurabayaDepartment of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, SurabayaDepartment of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, SurabayaDepartement of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, SurabayaDepartment of Microbiology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, SurabayaDepartment of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, SurabayaBackground: Oral candidiasis is an oral infection caused by Candida albicans. It is one of the most common opportunistic infections found in patients with Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS). Due to growing resistance and side effects to common antifungal drugs in recent years, there have been many studies on natural substances as antifungal agents. In this study, Epigallocatechin gallate (EGCG) were examined for in vitro antifungal activity against Candida strains. Purpose: To compare the in vitro antifungal activity of EGCG and nystatin towards Candida sp. isolate in HIV/AIDS patients with oral candidiasis. Methods: This research was an experimental laboratory study conducted at the Dr. Soetomo General Academic Hospital, Surabaya. Forty isolates of Candida albicans and Candida non-albicans were included. The isolates were tested for antifungal activity using the well diffusion and microdilution method for nystatin 1000 IU and EGCG 1.25%. Result: Diffusion test results showed a greater inhibition zone for nystatin against all analyzed Candida strains with an average diameter for Candida albicans formed by EGCG of 2.15 mm and 7.4 mm for Candida non-albicans. Meanwhile, based on the microdilution test, EGCG was better than nystatin towards all analyzed Candida strains. This study showed EGCG Minimum Fungicidal Concentration (MFC) as high as 50% (p < 0.05). Conclusion: Nystatin antifungal activity was better than EGCG supported by a greater inhibition zone in the well diffusion method. However, the antifungal activity of EGCG was better than nystatin based on microdilution methods.https://e-journal.unair.ac.id/BIKK/article/view/21775antifungalcandidanystatinepigallocatechin gallateaids/hiv |
| spellingShingle | Yusuf Wibisono Afif Nurul Hidayati Sawitri Sawitri Iskandar Zulkarnain Evy Ervianti Rahmadewi Rahmadewi Esti Hendradi Pepy Dwi Endraswari Dwi Murtiastutik In Vitro Comparison of Antifungal Activity between Epigallocatechin Gallate EGCG) and Nystatin on Candida Sp. Stored Isolates in HIV/AIDS Patients with Oral Candidiasis Berkala Ilmu Kesehatan Kulit dan Kelamin (Periodical of Dermatology and Venerology) antifungal candida nystatin epigallocatechin gallate aids/hiv |
| title | In Vitro Comparison of Antifungal Activity between Epigallocatechin Gallate EGCG) and Nystatin on Candida Sp. Stored Isolates in HIV/AIDS Patients with Oral Candidiasis |
| title_full | In Vitro Comparison of Antifungal Activity between Epigallocatechin Gallate EGCG) and Nystatin on Candida Sp. Stored Isolates in HIV/AIDS Patients with Oral Candidiasis |
| title_fullStr | In Vitro Comparison of Antifungal Activity between Epigallocatechin Gallate EGCG) and Nystatin on Candida Sp. Stored Isolates in HIV/AIDS Patients with Oral Candidiasis |
| title_full_unstemmed | In Vitro Comparison of Antifungal Activity between Epigallocatechin Gallate EGCG) and Nystatin on Candida Sp. Stored Isolates in HIV/AIDS Patients with Oral Candidiasis |
| title_short | In Vitro Comparison of Antifungal Activity between Epigallocatechin Gallate EGCG) and Nystatin on Candida Sp. Stored Isolates in HIV/AIDS Patients with Oral Candidiasis |
| title_sort | in vitro comparison of antifungal activity between epigallocatechin gallate egcg and nystatin on candida sp stored isolates in hiv aids patients with oral candidiasis |
| topic | antifungal candida nystatin epigallocatechin gallate aids/hiv |
| url | https://e-journal.unair.ac.id/BIKK/article/view/21775 |
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