In Vitro Differentiation of Embryonic Stem Cells Into Cardiac-Like and Neuronal-Like Cells
Embryonic stem cells are pluripotent cell lines with the capacity of self-renewal and a broad differentiation plastic-ity. They are isolated from preimplantation embryos and can be cultured in vitro for long time without losing their pluripo-tency. Embryonic stem cells can also differentiate in vitr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS)
2008-07-01
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| Series: | Clinical and Biomedical Research |
| Subjects: | |
| Online Access: | https://seer.ufrgs.br/index.php/hcpa/article/view/2435 |
| _version_ | 1818504544733102080 |
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| author | Ana Helena da Rosa Paz Ana Ayala Lugo Paula Barros Terraciano Luise Meurer Eduardo Pandolfi Passos Elizabeth Cirne Lima |
| author_facet | Ana Helena da Rosa Paz Ana Ayala Lugo Paula Barros Terraciano Luise Meurer Eduardo Pandolfi Passos Elizabeth Cirne Lima |
| author_sort | Ana Helena da Rosa Paz |
| collection | DOAJ |
| description | Embryonic stem cells are pluripotent cell lines with the capacity of self-renewal and a broad differentiation plastic-ity. They are isolated from preimplantation embryos and can be cultured in vitro for long time without losing their pluripo-tency. Embryonic stem cells can also differentiate in vitro with the proper combination of growth and differentiation fac-tors, cells will differentiate into more advanced stages of embryogenesis generating different adult cell type. In the pre-sent study, we induced the in vitro differentiation of mouse embryonic stem cells (line R1) into cardiomyocytes and neu-ronal cells. These differentiations were evaluated by reverse transcription-polymerase chain reaction to verify presence of tissue-specific markers. |
| first_indexed | 2024-12-10T21:38:29Z |
| format | Article |
| id | doaj.art-d15a5e435f9a46ae9f21bb04c4e27e9c |
| institution | Directory Open Access Journal |
| issn | 2357-9730 |
| language | English |
| last_indexed | 2024-12-10T21:38:29Z |
| publishDate | 2008-07-01 |
| publisher | Hospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS) |
| record_format | Article |
| series | Clinical and Biomedical Research |
| spelling | doaj.art-d15a5e435f9a46ae9f21bb04c4e27e9c2022-12-22T01:32:34ZengHospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS)Clinical and Biomedical Research2357-97302008-07-012823010In Vitro Differentiation of Embryonic Stem Cells Into Cardiac-Like and Neuronal-Like CellsAna Helena da Rosa Paz0Ana Ayala Lugo1Paula Barros Terraciano2Luise Meurer3Eduardo Pandolfi Passos4Elizabeth Cirne Lima5UFRGSPrograma de Pós Graduação em Genética e Biologia Celular- UFRGSHospital de Clínicas de Porto AlegreHospital de Clínicas de Porto Alegre - UFRGSUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulEmbryonic stem cells are pluripotent cell lines with the capacity of self-renewal and a broad differentiation plastic-ity. They are isolated from preimplantation embryos and can be cultured in vitro for long time without losing their pluripo-tency. Embryonic stem cells can also differentiate in vitro with the proper combination of growth and differentiation fac-tors, cells will differentiate into more advanced stages of embryogenesis generating different adult cell type. In the pre-sent study, we induced the in vitro differentiation of mouse embryonic stem cells (line R1) into cardiomyocytes and neu-ronal cells. These differentiations were evaluated by reverse transcription-polymerase chain reaction to verify presence of tissue-specific markers.https://seer.ufrgs.br/index.php/hcpa/article/view/2435Células-tronco embrionáriasdiferenciação in vitrocultivo de células-tronco embrionárias |
| spellingShingle | Ana Helena da Rosa Paz Ana Ayala Lugo Paula Barros Terraciano Luise Meurer Eduardo Pandolfi Passos Elizabeth Cirne Lima In Vitro Differentiation of Embryonic Stem Cells Into Cardiac-Like and Neuronal-Like Cells Clinical and Biomedical Research Células-tronco embrionárias diferenciação in vitro cultivo de células-tronco embrionárias |
| title | In Vitro Differentiation of Embryonic Stem Cells Into Cardiac-Like and Neuronal-Like Cells |
| title_full | In Vitro Differentiation of Embryonic Stem Cells Into Cardiac-Like and Neuronal-Like Cells |
| title_fullStr | In Vitro Differentiation of Embryonic Stem Cells Into Cardiac-Like and Neuronal-Like Cells |
| title_full_unstemmed | In Vitro Differentiation of Embryonic Stem Cells Into Cardiac-Like and Neuronal-Like Cells |
| title_short | In Vitro Differentiation of Embryonic Stem Cells Into Cardiac-Like and Neuronal-Like Cells |
| title_sort | in vitro differentiation of embryonic stem cells into cardiac like and neuronal like cells |
| topic | Células-tronco embrionárias diferenciação in vitro cultivo de células-tronco embrionárias |
| url | https://seer.ufrgs.br/index.php/hcpa/article/view/2435 |
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