Lycopene Synthesis and Related Gene Expression in Pummelo Pulp Increased in Shade-grown Fruit
Abstract The effects of bagging-induced light reductions on lycopene synthesis and the expression of related genes, antioxidant activity, and sugar composition of ‘Tubtim Siam’ pummelo (Citrus maxima) were investigated. Glucose, ascorbic acid, and flavonoid concentrations and 2, 2-diphenyl-1-picryh...
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| Format: | Article |
| Language: | English |
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American Society for Horticultural Science (ASHS)
2019-11-01
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| Series: | Journal of the American Society for Horticultural Science |
| Subjects: | |
| Online Access: | https://journals.ashs.org/jashs/view/journals/jashs/145/1/article-p60.xml |
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| author | Paemika Promkaew Varit Srilaong Chalermchai Wongs-Aree Nutthachai Pongprasert Samak Kaewsuksaeng Satoru Kondo |
| author_facet | Paemika Promkaew Varit Srilaong Chalermchai Wongs-Aree Nutthachai Pongprasert Samak Kaewsuksaeng Satoru Kondo |
| author_sort | Paemika Promkaew |
| collection | DOAJ |
| description | Abstract
The effects of bagging-induced light reductions on lycopene synthesis and the expression of related genes, antioxidant activity, and sugar composition of ‘Tubtim Siam’ pummelo (Citrus maxima) were investigated. Glucose, ascorbic acid, and flavonoid concentrations and 2, 2-diphenyl-1-picryhydrazyl scavenging activity were decreased in fruit covered with bags while still on the tree [0.01 μmol·m−2·s−1 photosynthetic photon flux density (PPFD)] compared with the untreated control (596.7 μmol·m−2·s−1 PPFD). The bagging treatment significantly decreased the temperatures on the surface in the bag. In addition, the bagging treatment decreased abscisic acid concentrations in the peel and pulp. However, the bagging treatment increased lycopene concentrations, upregulated phytoene synthase (CsPSY) and ζ-carotene desaturase (CsZDS) gene expressions; downregulated chromoplast-specific lycopene cyclase (CsβLCY), β-carotene hydroxylase (CsβCHX), and ε-ring hydroxylase (CsɛCHX); and decreased 9-cis-epoxycarotenoid dioxygenase (CsNCED1) gene expressions in the pulp. It is possible that maintaining a temperature of ≈25 °C in fruit covered with bags may increase the lycopene concentration in the pulp with the upregulation of CsPSY and CsZDS and the downregulation of CsβLCY, CsβCHX, CsɛCHX, and CsNCED1 gene expressions in the pulp. |
| first_indexed | 2024-04-14T01:58:24Z |
| format | Article |
| id | doaj.art-d15c30010a114eacbe98614efd4e80d8 |
| institution | Directory Open Access Journal |
| issn | 2327-9788 |
| language | English |
| last_indexed | 2024-04-14T01:58:24Z |
| publishDate | 2019-11-01 |
| publisher | American Society for Horticultural Science (ASHS) |
| record_format | Article |
| series | Journal of the American Society for Horticultural Science |
| spelling | doaj.art-d15c30010a114eacbe98614efd4e80d82022-12-22T02:18:56ZengAmerican Society for Horticultural Science (ASHS)Journal of the American Society for Horticultural Science2327-97882019-11-0114516066https://doi.org/10.21273/JASHS04814-19Lycopene Synthesis and Related Gene Expression in Pummelo Pulp Increased in Shade-grown FruitPaemika PromkaewVarit Srilaong Chalermchai Wongs-AreeNutthachai PongprasertSamak Kaewsuksaeng Satoru KondoAbstract The effects of bagging-induced light reductions on lycopene synthesis and the expression of related genes, antioxidant activity, and sugar composition of ‘Tubtim Siam’ pummelo (Citrus maxima) were investigated. Glucose, ascorbic acid, and flavonoid concentrations and 2, 2-diphenyl-1-picryhydrazyl scavenging activity were decreased in fruit covered with bags while still on the tree [0.01 μmol·m−2·s−1 photosynthetic photon flux density (PPFD)] compared with the untreated control (596.7 μmol·m−2·s−1 PPFD). The bagging treatment significantly decreased the temperatures on the surface in the bag. In addition, the bagging treatment decreased abscisic acid concentrations in the peel and pulp. However, the bagging treatment increased lycopene concentrations, upregulated phytoene synthase (CsPSY) and ζ-carotene desaturase (CsZDS) gene expressions; downregulated chromoplast-specific lycopene cyclase (CsβLCY), β-carotene hydroxylase (CsβCHX), and ε-ring hydroxylase (CsɛCHX); and decreased 9-cis-epoxycarotenoid dioxygenase (CsNCED1) gene expressions in the pulp. It is possible that maintaining a temperature of ≈25 °C in fruit covered with bags may increase the lycopene concentration in the pulp with the upregulation of CsPSY and CsZDS and the downregulation of CsβLCY, CsβCHX, CsɛCHX, and CsNCED1 gene expressions in the pulp.https://journals.ashs.org/jashs/view/journals/jashs/145/1/article-p60.xmlabscisic acid; bagging; carotenoid; citrus maxima; temperature |
| spellingShingle | Paemika Promkaew Varit Srilaong Chalermchai Wongs-Aree Nutthachai Pongprasert Samak Kaewsuksaeng Satoru Kondo Lycopene Synthesis and Related Gene Expression in Pummelo Pulp Increased in Shade-grown Fruit Journal of the American Society for Horticultural Science abscisic acid; bagging; carotenoid; citrus maxima; temperature |
| title | Lycopene Synthesis and Related Gene Expression in Pummelo Pulp Increased in Shade-grown Fruit |
| title_full | Lycopene Synthesis and Related Gene Expression in Pummelo Pulp Increased in Shade-grown Fruit |
| title_fullStr | Lycopene Synthesis and Related Gene Expression in Pummelo Pulp Increased in Shade-grown Fruit |
| title_full_unstemmed | Lycopene Synthesis and Related Gene Expression in Pummelo Pulp Increased in Shade-grown Fruit |
| title_short | Lycopene Synthesis and Related Gene Expression in Pummelo Pulp Increased in Shade-grown Fruit |
| title_sort | lycopene synthesis and related gene expression in pummelo pulp increased in shade grown fruit |
| topic | abscisic acid; bagging; carotenoid; citrus maxima; temperature |
| url | https://journals.ashs.org/jashs/view/journals/jashs/145/1/article-p60.xml |
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