Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam
Cell-free fetal DNA (cffDNA) from fetal calves was isolated in the bovine maternal plasma in the fifth week of pregnancy, accounting for around 10% of the total. Recently, cffDNA has been used as a non-invasive prenatal screening technique for aneuploidy, genetic disorders in humans, early...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Chiang Mai University
2023-05-01
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| Series: | Veterinary Integrative Sciences |
| Online Access: | https://he02.tci-thaijo.org/index.php/vis/article/view/263119 |
| _version_ | 1797664694480142336 |
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| author | Thi Thanh Khuong Tran Dang Khoa Nguyen Van Loi Nguyen Van Be Nam Tran Gia Huy Tran Trong Ngu Nguyen Phuoc Thanh Lam Thi Tai Nguyen Cao |
| author_facet | Thi Thanh Khuong Tran Dang Khoa Nguyen Van Loi Nguyen Van Be Nam Tran Gia Huy Tran Trong Ngu Nguyen Phuoc Thanh Lam Thi Tai Nguyen Cao |
| author_sort | Thi Thanh Khuong Tran |
| collection | DOAJ |
| description | Cell-free fetal DNA (cffDNA) from fetal calves was isolated in the bovine maternal plasma in the fifth week of pregnancy, accounting for around 10% of the total. Recently, cffDNA has been used as a non-invasive prenatal screening technique for aneuploidy, genetic disorders in humans, early pregnancy diagnosis, and animal fetal sex determination. This study's objective was to identify the presence of cffDNA in the blood of 13 dairy cows (11 pregnant, 2 non-pregnant, and 1 unmarred) gathered from farms in Can Tho, the Mekong Delta, Vietnam. Plasma / Serum cfc-DNA Purification Midi Kit (Product # 55600) was used to separate cffDNA. Two PCR-designed primers named SRY-137 and GAPDH-109 were employed to amplify the Y-chromosome-specific sequences SRY and the housekeeping gene GAPDH, respectively. The fetal sex was indicated by PCR data corresponding to the calf's sex at birth in 11 cases. The study's findings first proved the existence of cell-free fetal DNA circulating in the plasma of a pregnant cow in Vietnam and established the calf's sex using simple, high-precision procedures. The use of cffDNA for sex screening brings up the possibility of quickly determining the gender and screening for genetic diseases in a big herd of cattle and animals with long gestation periods. |
| first_indexed | 2024-03-11T19:33:05Z |
| format | Article |
| id | doaj.art-d15c77593c5149e9abf80810f37f49ea |
| institution | Directory Open Access Journal |
| issn | 2629-9968 |
| language | English |
| last_indexed | 2024-03-11T19:33:05Z |
| publishDate | 2023-05-01 |
| publisher | Faculty of Veterinary Medicine, Chiang Mai University |
| record_format | Article |
| series | Veterinary Integrative Sciences |
| spelling | doaj.art-d15c77593c5149e9abf80810f37f49ea2023-10-06T13:15:40ZengFaculty of Veterinary Medicine, Chiang Mai UniversityVeterinary Integrative Sciences2629-99682023-05-0121310.12982/VIS.2023.047Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, VietnamThi Thanh Khuong TranDang Khoa NguyenVan Loi NguyenVan Be Nam TranGia Huy TranTrong Ngu NguyenPhuoc Thanh LamThi Tai Nguyen Cao Cell-free fetal DNA (cffDNA) from fetal calves was isolated in the bovine maternal plasma in the fifth week of pregnancy, accounting for around 10% of the total. Recently, cffDNA has been used as a non-invasive prenatal screening technique for aneuploidy, genetic disorders in humans, early pregnancy diagnosis, and animal fetal sex determination. This study's objective was to identify the presence of cffDNA in the blood of 13 dairy cows (11 pregnant, 2 non-pregnant, and 1 unmarred) gathered from farms in Can Tho, the Mekong Delta, Vietnam. Plasma / Serum cfc-DNA Purification Midi Kit (Product # 55600) was used to separate cffDNA. Two PCR-designed primers named SRY-137 and GAPDH-109 were employed to amplify the Y-chromosome-specific sequences SRY and the housekeeping gene GAPDH, respectively. The fetal sex was indicated by PCR data corresponding to the calf's sex at birth in 11 cases. The study's findings first proved the existence of cell-free fetal DNA circulating in the plasma of a pregnant cow in Vietnam and established the calf's sex using simple, high-precision procedures. The use of cffDNA for sex screening brings up the possibility of quickly determining the gender and screening for genetic diseases in a big herd of cattle and animals with long gestation periods.https://he02.tci-thaijo.org/index.php/vis/article/view/263119 |
| spellingShingle | Thi Thanh Khuong Tran Dang Khoa Nguyen Van Loi Nguyen Van Be Nam Tran Gia Huy Tran Trong Ngu Nguyen Phuoc Thanh Lam Thi Tai Nguyen Cao Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam Veterinary Integrative Sciences |
| title | Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam |
| title_full | Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam |
| title_fullStr | Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam |
| title_full_unstemmed | Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam |
| title_short | Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam |
| title_sort | cattle fetal sex determination using cell free fetal dna from maternal blood in the mekong delta vietnam |
| url | https://he02.tci-thaijo.org/index.php/vis/article/view/263119 |
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