Exposure of <i>Aspergillus fumigatus</i> to <i>Klebsiella pneumoniae</i> Culture Filtrate Inhibits Growth and Stimulates Gliotoxin Production
<i>Aspergillus fumigatus</i> is an opportunistic fungal pathogen capable of inducing chronic and acute infection in susceptible patients. <i>A. fumigatus</i> interacts with numerous bacteria that compose the microbiota of the lung, including <i>Pseudomonas aeruginosa<...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/2/222 |
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| author | Aaron Curtis Michelle Ryan Kevin Kavanagh |
| author_facet | Aaron Curtis Michelle Ryan Kevin Kavanagh |
| author_sort | Aaron Curtis |
| collection | DOAJ |
| description | <i>Aspergillus fumigatus</i> is an opportunistic fungal pathogen capable of inducing chronic and acute infection in susceptible patients. <i>A. fumigatus</i> interacts with numerous bacteria that compose the microbiota of the lung, including <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i>, both of which are common isolates from cystic fibrosis sputum. Exposure of <i>A. fumigatus</i> to <i>K. pneumoniae</i> culture filtrate reduced fungal growth and increased gliotoxin production. Qualitative proteomic analysis of the <i>K. pneumoniae</i> culture filtrate identified proteins associated with metal sequestering, enzymatic degradation and redox activity, which may impact fungal growth and development. Quantitative proteomic analysis of <i>A. fumigatus</i> following exposure to <i>K. pneumoniae</i> culture filtrate (25% <i>v</i>/<i>v</i>) for 24 h revealed a reduced abundance of 1,3-beta-glucanosyltransferase (−3.97 fold), methyl sterol monooxygenase erg25B (−2.9 fold) and calcium/calmodulin-dependent protein kinase (−4.2 fold) involved in fungal development, and increased abundance of glutathione S-transferase GliG (+6.17 fold), non-ribosomal peptide synthase GliP (+3.67 fold), O-methyltransferase GliM (+3.5 fold), gamma-glutamyl acyltransferase GliK (+2.89 fold) and thioredoxin reductase GliT (+2.33 fold) involved in gliotoxin production. These results reveal that exposure of <i>A. fumigatus</i> to <i>K. pneumoniae</i> in vivo could exacerbate infection and negatively impact patient prognosis. |
| first_indexed | 2024-03-11T08:36:07Z |
| format | Article |
| id | doaj.art-d15c7dc5aa5d49cbbba39ce7cc739764 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T08:36:07Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-d15c7dc5aa5d49cbbba39ce7cc7397642023-11-16T21:30:46ZengMDPI AGJournal of Fungi2309-608X2023-02-019222210.3390/jof9020222Exposure of <i>Aspergillus fumigatus</i> to <i>Klebsiella pneumoniae</i> Culture Filtrate Inhibits Growth and Stimulates Gliotoxin ProductionAaron Curtis0Michelle Ryan1Kevin Kavanagh2Medical Mycology Unit, Department of Biology, Maynooth University, W23 F2H6 Co. Kildare, IrelandMedical Mycology Unit, Department of Biology, Maynooth University, W23 F2H6 Co. Kildare, IrelandMedical Mycology Unit, Department of Biology, Maynooth University, W23 F2H6 Co. Kildare, Ireland<i>Aspergillus fumigatus</i> is an opportunistic fungal pathogen capable of inducing chronic and acute infection in susceptible patients. <i>A. fumigatus</i> interacts with numerous bacteria that compose the microbiota of the lung, including <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i>, both of which are common isolates from cystic fibrosis sputum. Exposure of <i>A. fumigatus</i> to <i>K. pneumoniae</i> culture filtrate reduced fungal growth and increased gliotoxin production. Qualitative proteomic analysis of the <i>K. pneumoniae</i> culture filtrate identified proteins associated with metal sequestering, enzymatic degradation and redox activity, which may impact fungal growth and development. Quantitative proteomic analysis of <i>A. fumigatus</i> following exposure to <i>K. pneumoniae</i> culture filtrate (25% <i>v</i>/<i>v</i>) for 24 h revealed a reduced abundance of 1,3-beta-glucanosyltransferase (−3.97 fold), methyl sterol monooxygenase erg25B (−2.9 fold) and calcium/calmodulin-dependent protein kinase (−4.2 fold) involved in fungal development, and increased abundance of glutathione S-transferase GliG (+6.17 fold), non-ribosomal peptide synthase GliP (+3.67 fold), O-methyltransferase GliM (+3.5 fold), gamma-glutamyl acyltransferase GliK (+2.89 fold) and thioredoxin reductase GliT (+2.33 fold) involved in gliotoxin production. These results reveal that exposure of <i>A. fumigatus</i> to <i>K. pneumoniae</i> in vivo could exacerbate infection and negatively impact patient prognosis.https://www.mdpi.com/2309-608X/9/2/222<i>Aspergillus fumigatus</i><i>Klebsiella pneumoniae</i>fungal–bacterial interactiongliotoxinproteomics |
| spellingShingle | Aaron Curtis Michelle Ryan Kevin Kavanagh Exposure of <i>Aspergillus fumigatus</i> to <i>Klebsiella pneumoniae</i> Culture Filtrate Inhibits Growth and Stimulates Gliotoxin Production Journal of Fungi <i>Aspergillus fumigatus</i> <i>Klebsiella pneumoniae</i> fungal–bacterial interaction gliotoxin proteomics |
| title | Exposure of <i>Aspergillus fumigatus</i> to <i>Klebsiella pneumoniae</i> Culture Filtrate Inhibits Growth and Stimulates Gliotoxin Production |
| title_full | Exposure of <i>Aspergillus fumigatus</i> to <i>Klebsiella pneumoniae</i> Culture Filtrate Inhibits Growth and Stimulates Gliotoxin Production |
| title_fullStr | Exposure of <i>Aspergillus fumigatus</i> to <i>Klebsiella pneumoniae</i> Culture Filtrate Inhibits Growth and Stimulates Gliotoxin Production |
| title_full_unstemmed | Exposure of <i>Aspergillus fumigatus</i> to <i>Klebsiella pneumoniae</i> Culture Filtrate Inhibits Growth and Stimulates Gliotoxin Production |
| title_short | Exposure of <i>Aspergillus fumigatus</i> to <i>Klebsiella pneumoniae</i> Culture Filtrate Inhibits Growth and Stimulates Gliotoxin Production |
| title_sort | exposure of i aspergillus fumigatus i to i klebsiella pneumoniae i culture filtrate inhibits growth and stimulates gliotoxin production |
| topic | <i>Aspergillus fumigatus</i> <i>Klebsiella pneumoniae</i> fungal–bacterial interaction gliotoxin proteomics |
| url | https://www.mdpi.com/2309-608X/9/2/222 |
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