Ultrastructural study of sperm cells in Acanthocolpidae: the case of Stephanostomum murielae and Stephanostomoides tenuis (Digenea)
The mature spermatozoa of Stephanostomum murielae and Stephanostomoides tenuis are described by transmission electron microscopy. They present several ultrastructural features previously reported in other digeneans. Their spermatozoa possess two axonemes of different length showing the 9 + ‘1’ trepa...
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PeerJ Inc.
2015-02-01
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| author | Abdoulaye J.S. Bakhoum Yann Quilichini Jean-Lou Justine Rodney A. Bray Cheikh T. Bâ Bernard Marchand |
| author_facet | Abdoulaye J.S. Bakhoum Yann Quilichini Jean-Lou Justine Rodney A. Bray Cheikh T. Bâ Bernard Marchand |
| author_sort | Abdoulaye J.S. Bakhoum |
| collection | DOAJ |
| description | The mature spermatozoa of Stephanostomum murielae and Stephanostomoides tenuis are described by transmission electron microscopy. They present several ultrastructural features previously reported in other digeneans. Their spermatozoa possess two axonemes of different length showing the 9 + ‘1’ trepaxonematan pattern, four attachment zones, two mitochondria (with an anterior moniliform one in S. murielae), a nucleus, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, spine-like bodies and granules of glycogen. The main differences between the mature spermatozoon of S. murielae and S. tenuis are the maximum number of cortical microtubules, the morphology of the anterior spermatozoon extremity and the anterior mitochondrion. This study is the first concerning members of the family Acanthocolpidae. The main ultrastructural characteristics discussed are the morphology of the anterior and posterior spermatozoon extremities, antero-lateral electron dense material, external ornamentations, spine-like bodies and number and morphology of mitochondria. In addition, the phylogenetic significance of all these ultrastructural features is discussed and compared to molecular results in order to highlight the complex relationships in the Digenea. |
| first_indexed | 2024-03-09T07:24:26Z |
| format | Article |
| id | doaj.art-d15de61bc6294de180eb0989dc2b86d9 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:24:26Z |
| publishDate | 2015-02-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-d15de61bc6294de180eb0989dc2b86d92023-12-03T07:09:00ZengPeerJ Inc.PeerJ2167-83592015-02-013e74410.7717/peerj.744744Ultrastructural study of sperm cells in Acanthocolpidae: the case of Stephanostomum murielae and Stephanostomoides tenuis (Digenea)Abdoulaye J.S. Bakhoum0Yann Quilichini1Jean-Lou Justine2Rodney A. Bray3Cheikh T. Bâ4Bernard Marchand5SERME Service d’Étude et de Recherche en Microscopie Electronique, UMR 6134-SPE CNRS—Università di Corsica, Corte, Corsica, FranceSERME Service d’Étude et de Recherche en Microscopie Electronique, UMR 6134-SPE CNRS—Università di Corsica, Corte, Corsica, FranceISYEB, Institut de Systématique, Évolution, Biodiversité (UMR7205 CNRS, EPHE, MNHN, UPMC), Muséum National d’Histoire Naturelle, Paris Cedex, FranceDepartment of Life Sciences, Natural History Museum, London, United KingdomLaboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Cheikh Anta Diop University of Dakar, Dakar, SenegalSERME Service d’Étude et de Recherche en Microscopie Electronique, UMR 6134-SPE CNRS—Università di Corsica, Corte, Corsica, FranceThe mature spermatozoa of Stephanostomum murielae and Stephanostomoides tenuis are described by transmission electron microscopy. They present several ultrastructural features previously reported in other digeneans. Their spermatozoa possess two axonemes of different length showing the 9 + ‘1’ trepaxonematan pattern, four attachment zones, two mitochondria (with an anterior moniliform one in S. murielae), a nucleus, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, spine-like bodies and granules of glycogen. The main differences between the mature spermatozoon of S. murielae and S. tenuis are the maximum number of cortical microtubules, the morphology of the anterior spermatozoon extremity and the anterior mitochondrion. This study is the first concerning members of the family Acanthocolpidae. The main ultrastructural characteristics discussed are the morphology of the anterior and posterior spermatozoon extremities, antero-lateral electron dense material, external ornamentations, spine-like bodies and number and morphology of mitochondria. In addition, the phylogenetic significance of all these ultrastructural features is discussed and compared to molecular results in order to highlight the complex relationships in the Digenea.https://peerj.com/articles/744.pdfPlatyhelminthesSperm cellsTrematodaPhylogenyTransmission electron microscopy |
| spellingShingle | Abdoulaye J.S. Bakhoum Yann Quilichini Jean-Lou Justine Rodney A. Bray Cheikh T. Bâ Bernard Marchand Ultrastructural study of sperm cells in Acanthocolpidae: the case of Stephanostomum murielae and Stephanostomoides tenuis (Digenea) PeerJ Platyhelminthes Sperm cells Trematoda Phylogeny Transmission electron microscopy |
| title | Ultrastructural study of sperm cells in Acanthocolpidae: the case of Stephanostomum murielae and Stephanostomoides tenuis (Digenea) |
| title_full | Ultrastructural study of sperm cells in Acanthocolpidae: the case of Stephanostomum murielae and Stephanostomoides tenuis (Digenea) |
| title_fullStr | Ultrastructural study of sperm cells in Acanthocolpidae: the case of Stephanostomum murielae and Stephanostomoides tenuis (Digenea) |
| title_full_unstemmed | Ultrastructural study of sperm cells in Acanthocolpidae: the case of Stephanostomum murielae and Stephanostomoides tenuis (Digenea) |
| title_short | Ultrastructural study of sperm cells in Acanthocolpidae: the case of Stephanostomum murielae and Stephanostomoides tenuis (Digenea) |
| title_sort | ultrastructural study of sperm cells in acanthocolpidae the case of stephanostomum murielae and stephanostomoides tenuis digenea |
| topic | Platyhelminthes Sperm cells Trematoda Phylogeny Transmission electron microscopy |
| url | https://peerj.com/articles/744.pdf |
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