Expression of Hsp70 and Caspase-3 in rabbits after severe traumatic brain injury
【Abstract】 Objective: To investigate the expres-sion of Caspase-3 and Hsp70 in rabbits after severe trau-matic brain injury (TBI) and to explore the feasibility of its application in estimation of injury time in forensic medicine. Methods: A rabbit model of heavy TBI was developed by high v...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-12-01
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| Series: | Chinese Journal of Traumatology |
| Online Access: | http://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/335 |
| _version_ | 1811338831999270912 |
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| author | ZHANG Jing TAO Dai-qin ZHAO Hui YIN Zhi-yong |
| author_facet | ZHANG Jing TAO Dai-qin ZHAO Hui YIN Zhi-yong |
| author_sort | ZHANG Jing |
| collection | DOAJ |
| description | 【Abstract】 Objective: To investigate the expres-sion of Caspase-3 and Hsp70 in rabbits after severe trau-matic brain injury (TBI) and to explore the feasibility of its
application in estimation of injury time in forensic medicine.
Methods: A rabbit model of heavy TBI was developed
by high velocity impact on the parietal bone with an iron
stick. Totally 8 healthy adult New Zealand white rabbits
were randomly divided into control group (n=2) and injury
group (n=6). Four hours after injury, tissue specimens from
the parietal lobe, temporal lobe, occipital lobe, cerebellum
and brainstem were harvested to detect the expression of
Hsp70 and Caspase-3 by immunohistochemistry. Besides,
the gray values of cells positive for Hsp70 and Caspase-3
were analyzed with an image analyzer.
Results: Immunohistochemistry staining demonstrated
a low level of Caspase-3 and Hsp70 expression in normal
control group. While in injury group, both the Caspase-3
and Hsp70 expression was significantly elevated (P<0.05).
Positive cells gathered around the lesion focus. Occipital
lobe and cerebellum had fewer positive cells while temporal
and brainstem had the fewest.
Conclusion: The expression of Caspase-3 and Hsp70
at an early stage following severe TBI is characteristic and
can be applied to estimate the time of injury.
Key words: Forensic sciences; Craniocerebral
trauma; Caspase-3; HSP70 Heat-shock proteins; Immu-nohistochemistry |
| first_indexed | 2024-04-13T18:16:26Z |
| format | Article |
| id | doaj.art-d15f2be7819d4b7c9fc345dddafd9db4 |
| institution | Directory Open Access Journal |
| issn | 1008-1275 |
| language | English |
| last_indexed | 2024-04-13T18:16:26Z |
| publishDate | 2012-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Chinese Journal of Traumatology |
| spelling | doaj.art-d15f2be7819d4b7c9fc345dddafd9db42022-12-22T02:35:40ZengElsevierChinese Journal of Traumatology1008-12752012-12-01156338341182Expression of Hsp70 and Caspase-3 in rabbits after severe traumatic brain injuryZHANG JingTAO Dai-qinZHAO HuiYIN Zhi-yong【Abstract】 Objective: To investigate the expres-sion of Caspase-3 and Hsp70 in rabbits after severe trau-matic brain injury (TBI) and to explore the feasibility of its application in estimation of injury time in forensic medicine. Methods: A rabbit model of heavy TBI was developed by high velocity impact on the parietal bone with an iron stick. Totally 8 healthy adult New Zealand white rabbits were randomly divided into control group (n=2) and injury group (n=6). Four hours after injury, tissue specimens from the parietal lobe, temporal lobe, occipital lobe, cerebellum and brainstem were harvested to detect the expression of Hsp70 and Caspase-3 by immunohistochemistry. Besides, the gray values of cells positive for Hsp70 and Caspase-3 were analyzed with an image analyzer. Results: Immunohistochemistry staining demonstrated a low level of Caspase-3 and Hsp70 expression in normal control group. While in injury group, both the Caspase-3 and Hsp70 expression was significantly elevated (P<0.05). Positive cells gathered around the lesion focus. Occipital lobe and cerebellum had fewer positive cells while temporal and brainstem had the fewest. Conclusion: The expression of Caspase-3 and Hsp70 at an early stage following severe TBI is characteristic and can be applied to estimate the time of injury. Key words: Forensic sciences; Craniocerebral trauma; Caspase-3; HSP70 Heat-shock proteins; Immu-nohistochemistryhttp://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/335 |
| spellingShingle | ZHANG Jing TAO Dai-qin ZHAO Hui YIN Zhi-yong Expression of Hsp70 and Caspase-3 in rabbits after severe traumatic brain injury Chinese Journal of Traumatology |
| title | Expression of Hsp70 and Caspase-3 in rabbits after severe traumatic brain injury |
| title_full | Expression of Hsp70 and Caspase-3 in rabbits after severe traumatic brain injury |
| title_fullStr | Expression of Hsp70 and Caspase-3 in rabbits after severe traumatic brain injury |
| title_full_unstemmed | Expression of Hsp70 and Caspase-3 in rabbits after severe traumatic brain injury |
| title_short | Expression of Hsp70 and Caspase-3 in rabbits after severe traumatic brain injury |
| title_sort | expression of hsp70 and caspase 3 in rabbits after severe traumatic brain injury |
| url | http://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/335 |
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