Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing
Abstract Background Triatomines are hematophagous insects that play an important role as vectors of Trypanosoma cruzi, the causative agent of Chagas disease. These insects have adapted to multiple blood-feeding sources that can affect relevant aspects of their life-cycle and interactions, thereby in...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-020-04310-z |
| _version_ | 1818477347684220928 |
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| author | Luisa M. Arias-Giraldo Marina Muñoz Carolina Hernández Giovanny Herrera Natalia Velásquez-Ortiz Omar Cantillo-Barraza Plutarco Urbano Andrés Cuervo Juan David Ramírez |
| author_facet | Luisa M. Arias-Giraldo Marina Muñoz Carolina Hernández Giovanny Herrera Natalia Velásquez-Ortiz Omar Cantillo-Barraza Plutarco Urbano Andrés Cuervo Juan David Ramírez |
| author_sort | Luisa M. Arias-Giraldo |
| collection | DOAJ |
| description | Abstract Background Triatomines are hematophagous insects that play an important role as vectors of Trypanosoma cruzi, the causative agent of Chagas disease. These insects have adapted to multiple blood-feeding sources that can affect relevant aspects of their life-cycle and interactions, thereby influencing parasitic transmission dynamics. We conducted a characterization of the feeding sources of individuals from the primary circulating triatomine genera in Colombia using amplicon-based next-generation sequencing (NGS). Methods We used 42 triatomines collected in different departments of Colombia. DNA was extracted from the gut. The presence of T. cruzi was identified using real-time PCR, and discrete typing units (DTUs) were determined by conventional PCR. For blood-feeding source identification, PCR products of the vertebrate 12S rRNA gene were obtained and sequenced by next-generation sequencing (NGS). Blood-meal sources were inferred using blastn against a curated reference dataset containing the 12S rRNA sequences belonging to vertebrates with a distribution in South America that represent a potential feeding source for triatomine bugs. Mean and median comparison tests were performed to evaluate differences in triatomine blood-feeding sources, infection state, and geographical regions. Lastly, the inverse Simpsonʼs diversity index was calculated. Results The overall frequency of T. cruzi infection was 83.3%. TcI was found as the most predominant DTU (65.7%). A total of 67 feeding sources were detected from the analyses of approximately 7 million reads. The predominant feeding source found was Homo sapiens (76.8%), followed by birds (10.5%), artiodactyls (4.4%), and non-human primates (3.9%). There were differences among numerous feeding sources of triatomines of different species. The diversity of feeding sources also differed depending on the presence of T. cruzi. Conclusions To the best of our knowledge, this is the first study to employ amplicon-based NGS of the 12S rRNA gene to depict blood-feeding sources of multiple triatomine species collected in different regions of Colombia. Our findings report a striking read diversity that has not been reported previously. This is a powerful approach to unravel transmission dynamics at microgeographical levels. |
| first_indexed | 2024-12-10T09:36:56Z |
| format | Article |
| id | doaj.art-d1652b3d4212498c99aa221e6396cb99 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-10T09:36:56Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-d1652b3d4212498c99aa221e6396cb992022-12-22T01:54:09ZengBMCParasites & Vectors1756-33052020-08-0113111410.1186/s13071-020-04310-zIdentification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencingLuisa M. Arias-Giraldo0Marina Muñoz1Carolina Hernández2Giovanny Herrera3Natalia Velásquez-Ortiz4Omar Cantillo-Barraza5Plutarco Urbano6Andrés Cuervo7Juan David Ramírez8Grupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del RosarioGrupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del RosarioGrupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del RosarioGrupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del RosarioGrupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del RosarioGrupo de Biología y Control de Enfermedades Infecciosas, Universidad de AntioquiaGrupo de Investigaciones Biológicas de la Orinoquia, Fundación Universitaria Internacional del Trópico Americano (Unitropico)Secretaría Departamental de Salud de AraucaGrupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del RosarioAbstract Background Triatomines are hematophagous insects that play an important role as vectors of Trypanosoma cruzi, the causative agent of Chagas disease. These insects have adapted to multiple blood-feeding sources that can affect relevant aspects of their life-cycle and interactions, thereby influencing parasitic transmission dynamics. We conducted a characterization of the feeding sources of individuals from the primary circulating triatomine genera in Colombia using amplicon-based next-generation sequencing (NGS). Methods We used 42 triatomines collected in different departments of Colombia. DNA was extracted from the gut. The presence of T. cruzi was identified using real-time PCR, and discrete typing units (DTUs) were determined by conventional PCR. For blood-feeding source identification, PCR products of the vertebrate 12S rRNA gene were obtained and sequenced by next-generation sequencing (NGS). Blood-meal sources were inferred using blastn against a curated reference dataset containing the 12S rRNA sequences belonging to vertebrates with a distribution in South America that represent a potential feeding source for triatomine bugs. Mean and median comparison tests were performed to evaluate differences in triatomine blood-feeding sources, infection state, and geographical regions. Lastly, the inverse Simpsonʼs diversity index was calculated. Results The overall frequency of T. cruzi infection was 83.3%. TcI was found as the most predominant DTU (65.7%). A total of 67 feeding sources were detected from the analyses of approximately 7 million reads. The predominant feeding source found was Homo sapiens (76.8%), followed by birds (10.5%), artiodactyls (4.4%), and non-human primates (3.9%). There were differences among numerous feeding sources of triatomines of different species. The diversity of feeding sources also differed depending on the presence of T. cruzi. Conclusions To the best of our knowledge, this is the first study to employ amplicon-based NGS of the 12S rRNA gene to depict blood-feeding sources of multiple triatomine species collected in different regions of Colombia. Our findings report a striking read diversity that has not been reported previously. This is a powerful approach to unravel transmission dynamics at microgeographical levels.http://link.springer.com/article/10.1186/s13071-020-04310-zChagas diseaseTrypanosoma cruziTriatominaeFeeding sourcesNGSColombia |
| spellingShingle | Luisa M. Arias-Giraldo Marina Muñoz Carolina Hernández Giovanny Herrera Natalia Velásquez-Ortiz Omar Cantillo-Barraza Plutarco Urbano Andrés Cuervo Juan David Ramírez Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing Parasites & Vectors Chagas disease Trypanosoma cruzi Triatominae Feeding sources NGS Colombia |
| title | Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing |
| title_full | Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing |
| title_fullStr | Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing |
| title_full_unstemmed | Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing |
| title_short | Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing |
| title_sort | identification of blood feeding sources in panstrongylus psammolestes rhodnius and triatoma using amplicon based next generation sequencing |
| topic | Chagas disease Trypanosoma cruzi Triatominae Feeding sources NGS Colombia |
| url | http://link.springer.com/article/10.1186/s13071-020-04310-z |
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