Fully autonomous mouse behavioral and optogenetic experiments in home-cage
Goal-directed behaviors involve distributed brain networks. The small size of the mouse brain makes it amenable to manipulations of neural activity dispersed across brain areas, but existing optogenetic methods serially test a few brain regions at a time, which slows comprehensive mapping of distrib...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/66112 |
| _version_ | 1811180375727144960 |
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| author | Yaoyao Hao Alyse Marian Thomas Nuo Li |
| author_facet | Yaoyao Hao Alyse Marian Thomas Nuo Li |
| author_sort | Yaoyao Hao |
| collection | DOAJ |
| description | Goal-directed behaviors involve distributed brain networks. The small size of the mouse brain makes it amenable to manipulations of neural activity dispersed across brain areas, but existing optogenetic methods serially test a few brain regions at a time, which slows comprehensive mapping of distributed networks. Laborious operant conditioning training required for most experimental paradigms exacerbates this bottleneck. We present an autonomous workflow to survey the involvement of brain regions at scale during operant behaviors in mice. Naive mice living in a home-cage system learned voluntary head-fixation (>1 hr/day) and performed difficult decision-making tasks, including contingency reversals, for 2 months without human supervision. We incorporated an optogenetic approach to manipulate activity in deep brain regions through intact skull during home-cage behavior. To demonstrate the utility of this approach, we tested dozens of mice in parallel unsupervised optogenetic experiments, revealing multiple regions in cortex, striatum, and superior colliculus involved in tactile decision-making. |
| first_indexed | 2024-04-11T09:02:13Z |
| format | Article |
| id | doaj.art-d1699904829848a3b2058179e3bfa859 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:02:13Z |
| publishDate | 2021-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d1699904829848a3b2058179e3bfa8592022-12-22T04:32:45ZengeLife Sciences Publications LtdeLife2050-084X2021-05-011010.7554/eLife.66112Fully autonomous mouse behavioral and optogenetic experiments in home-cageYaoyao Hao0Alyse Marian Thomas1Nuo Li2https://orcid.org/0000-0002-6613-5018Department of Neuroscience, Baylor College of Medicine, Houston, United StatesDepartment of Neuroscience, Baylor College of Medicine, Houston, United StatesDepartment of Neuroscience, Baylor College of Medicine, Houston, United StatesGoal-directed behaviors involve distributed brain networks. The small size of the mouse brain makes it amenable to manipulations of neural activity dispersed across brain areas, but existing optogenetic methods serially test a few brain regions at a time, which slows comprehensive mapping of distributed networks. Laborious operant conditioning training required for most experimental paradigms exacerbates this bottleneck. We present an autonomous workflow to survey the involvement of brain regions at scale during operant behaviors in mice. Naive mice living in a home-cage system learned voluntary head-fixation (>1 hr/day) and performed difficult decision-making tasks, including contingency reversals, for 2 months without human supervision. We incorporated an optogenetic approach to manipulate activity in deep brain regions through intact skull during home-cage behavior. To demonstrate the utility of this approach, we tested dozens of mice in parallel unsupervised optogenetic experiments, revealing multiple regions in cortex, striatum, and superior colliculus involved in tactile decision-making.https://elifesciences.org/articles/66112automated trainingoptogeneticsdecision-makingcortexstriatumsuperior colliculus |
| spellingShingle | Yaoyao Hao Alyse Marian Thomas Nuo Li Fully autonomous mouse behavioral and optogenetic experiments in home-cage eLife automated training optogenetics decision-making cortex striatum superior colliculus |
| title | Fully autonomous mouse behavioral and optogenetic experiments in home-cage |
| title_full | Fully autonomous mouse behavioral and optogenetic experiments in home-cage |
| title_fullStr | Fully autonomous mouse behavioral and optogenetic experiments in home-cage |
| title_full_unstemmed | Fully autonomous mouse behavioral and optogenetic experiments in home-cage |
| title_short | Fully autonomous mouse behavioral and optogenetic experiments in home-cage |
| title_sort | fully autonomous mouse behavioral and optogenetic experiments in home cage |
| topic | automated training optogenetics decision-making cortex striatum superior colliculus |
| url | https://elifesciences.org/articles/66112 |
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