Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication
Peroxiredoxins are a family of antioxidant proteins that protect cells from oxidative damage caused by reactive oxygen species (ROS). Herein, the peroxiredoxin 3 gene from grass carp (<i>Ctenopharyngodon idellus</i>), named <i>CiPrx3</i>, was cloned and analyzed. The full-len...
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1952 |
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| author | Xinyu Liang Yongming Li Pengfei Chu Qian Wang Hanyue Wang Lanjie Liao Cheng Yang Zuoyan Zhu Yaping Wang Libo He |
| author_facet | Xinyu Liang Yongming Li Pengfei Chu Qian Wang Hanyue Wang Lanjie Liao Cheng Yang Zuoyan Zhu Yaping Wang Libo He |
| author_sort | Xinyu Liang |
| collection | DOAJ |
| description | Peroxiredoxins are a family of antioxidant proteins that protect cells from oxidative damage caused by reactive oxygen species (ROS). Herein, the peroxiredoxin 3 gene from grass carp (<i>Ctenopharyngodon idellus</i>), named <i>CiPrx3</i>, was cloned and analyzed. The full-length cDNA of <i>CiPrx3</i> is 1068 bp long, with a 753 bp open reading frame (ORF) that contains a thioredoxin-2 domain, two peroxiredoxin signature motifs, and two highly conserved cysteine residues. <i>CiPrx3</i> was ubiquitously expressed in all the tested tissues, while its expression level was altered significantly after exposure to grass carp reovirus (GCRV) and pathogen-associated molecular patterns (PAMPs). <i>CiPrx3</i> was localized in the mitochondria of transfected cells and concentrated in the nucleus after poly (I:C) treatment. Transformation of <i>CiPrx3</i> into <i>Escherichia coli</i> enhanced host resistance to H<sub>2</sub>O<sub>2</sub> and heavy metals. Purified recombinant <i>CiPrx3</i> proteins could protect DNA against oxidative damage. Overexpression of <i>CiPrx3</i> in fish cells reduced intracellular ROS, increased cell viability, and decreased cell apoptosis caused by H<sub>2</sub>O<sub>2</sub> stimulation and GCRV infection. Further study indicated that <i>CiPrx3</i> induced autophagy to inhibit GCRV replication in fish cells. Collectively, these results imply that grass carp Prx3 elevates host antioxidant activity and induces autophagy to inhibit GCRV replication. |
| first_indexed | 2024-03-09T20:49:56Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:49:56Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-d171a12776dd483398de542e1202d1462023-11-23T22:38:23ZengMDPI AGAntioxidants2076-39212022-09-011110195210.3390/antiox11101952Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) ReplicationXinyu Liang0Yongming Li1Pengfei Chu2Qian Wang3Hanyue Wang4Lanjie Liao5Cheng Yang6Zuoyan Zhu7Yaping Wang8Libo He9State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaPeroxiredoxins are a family of antioxidant proteins that protect cells from oxidative damage caused by reactive oxygen species (ROS). Herein, the peroxiredoxin 3 gene from grass carp (<i>Ctenopharyngodon idellus</i>), named <i>CiPrx3</i>, was cloned and analyzed. The full-length cDNA of <i>CiPrx3</i> is 1068 bp long, with a 753 bp open reading frame (ORF) that contains a thioredoxin-2 domain, two peroxiredoxin signature motifs, and two highly conserved cysteine residues. <i>CiPrx3</i> was ubiquitously expressed in all the tested tissues, while its expression level was altered significantly after exposure to grass carp reovirus (GCRV) and pathogen-associated molecular patterns (PAMPs). <i>CiPrx3</i> was localized in the mitochondria of transfected cells and concentrated in the nucleus after poly (I:C) treatment. Transformation of <i>CiPrx3</i> into <i>Escherichia coli</i> enhanced host resistance to H<sub>2</sub>O<sub>2</sub> and heavy metals. Purified recombinant <i>CiPrx3</i> proteins could protect DNA against oxidative damage. Overexpression of <i>CiPrx3</i> in fish cells reduced intracellular ROS, increased cell viability, and decreased cell apoptosis caused by H<sub>2</sub>O<sub>2</sub> stimulation and GCRV infection. Further study indicated that <i>CiPrx3</i> induced autophagy to inhibit GCRV replication in fish cells. Collectively, these results imply that grass carp Prx3 elevates host antioxidant activity and induces autophagy to inhibit GCRV replication.https://www.mdpi.com/2076-3921/11/10/1952grass carpperoxiredoxin 3grass carp reovirusvirus replicationROSantioxidant |
| spellingShingle | Xinyu Liang Yongming Li Pengfei Chu Qian Wang Hanyue Wang Lanjie Liao Cheng Yang Zuoyan Zhu Yaping Wang Libo He Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication Antioxidants grass carp peroxiredoxin 3 grass carp reovirus virus replication ROS antioxidant |
| title | Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication |
| title_full | Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication |
| title_fullStr | Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication |
| title_full_unstemmed | Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication |
| title_short | Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication |
| title_sort | grass carp prx 3 elevates host antioxidant activity and induces autophagy to inhibit grass carp reovirus gcrv replication |
| topic | grass carp peroxiredoxin 3 grass carp reovirus virus replication ROS antioxidant |
| url | https://www.mdpi.com/2076-3921/11/10/1952 |
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