Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors
Lactoferrin (LF) exhibits a wide range of immunomodulatory activities including modulation of cytokine and chemokine secretion. In this study, we demonstrate that bovine LF (bLF) up-modulates, in a concentration- and time-dependent manner, CCL1 secretion in monocytes (Mo) at the early stage of diffe...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/7/12/4897 |
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| author | Daniela Latorre Nadia Pulvirenti Daniela Angela Covino Barbara Varano Cristina Purificato Gabriella Rainaldi Maria Cristina Gauzzi Laura Fantuzzi Lucia Conti Gloria Donninelli Manuela Del Cornò Michela Sabbatucci Sandra Gessani Patrizia Puddu |
| author_facet | Daniela Latorre Nadia Pulvirenti Daniela Angela Covino Barbara Varano Cristina Purificato Gabriella Rainaldi Maria Cristina Gauzzi Laura Fantuzzi Lucia Conti Gloria Donninelli Manuela Del Cornò Michela Sabbatucci Sandra Gessani Patrizia Puddu |
| author_sort | Daniela Latorre |
| collection | DOAJ |
| description | Lactoferrin (LF) exhibits a wide range of immunomodulatory activities including modulation of cytokine and chemokine secretion. In this study, we demonstrate that bovine LF (bLF) up-modulates, in a concentration- and time-dependent manner, CCL1 secretion in monocytes (Mo) at the early stage of differentiation toward dendritic cells (DCs), and in fully differentiated immature Mo-derived DCs (MoDCs). In both cell types, up-modulation of CCL1 secretion is an early event following bLF-mediated enhanced accumulation of CCL1 transcripts. Notably, bLF-mediated up-regulation of CCL1 involves the engagement of distinct surface receptors in MoDCs and their Mo precursors. We show that bLF-mediated engagement of CD36 contributes to CCL1 induction in differentiating Mo. Conversely, toll-like receptor (TLR)2 blocking markedly reduces bLF-induced CCL1 production in MoDCs. These findings add further evidence for cell-specific differential responses elicited by bLF through the engagement of distinct TLRs and surface receptors. Furthermore, the different responses observed at early and late stages of Mo differentiation towards DCs may be relevant in mediating bLF effects in specific body districts, where these cell types may be differently represented in physiopathological conditions. |
| first_indexed | 2024-04-11T22:15:47Z |
| format | Article |
| id | doaj.art-d173f1c573f647caaccd6c6aa1ee335d |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T22:15:47Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d173f1c573f647caaccd6c6aa1ee335d2022-12-22T04:00:26ZengMDPI AGToxins2072-66512015-12-017125472548310.3390/toxins7124897toxins7124897Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte PrecursorsDaniela Latorre0Nadia Pulvirenti1Daniela Angela Covino2Barbara Varano3Cristina Purificato4Gabriella Rainaldi5Maria Cristina Gauzzi6Laura Fantuzzi7Lucia Conti8Gloria Donninelli9Manuela Del Cornò10Michela Sabbatucci11Sandra Gessani12Patrizia Puddu13Istituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyIstituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, ItalyLactoferrin (LF) exhibits a wide range of immunomodulatory activities including modulation of cytokine and chemokine secretion. In this study, we demonstrate that bovine LF (bLF) up-modulates, in a concentration- and time-dependent manner, CCL1 secretion in monocytes (Mo) at the early stage of differentiation toward dendritic cells (DCs), and in fully differentiated immature Mo-derived DCs (MoDCs). In both cell types, up-modulation of CCL1 secretion is an early event following bLF-mediated enhanced accumulation of CCL1 transcripts. Notably, bLF-mediated up-regulation of CCL1 involves the engagement of distinct surface receptors in MoDCs and their Mo precursors. We show that bLF-mediated engagement of CD36 contributes to CCL1 induction in differentiating Mo. Conversely, toll-like receptor (TLR)2 blocking markedly reduces bLF-induced CCL1 production in MoDCs. These findings add further evidence for cell-specific differential responses elicited by bLF through the engagement of distinct TLRs and surface receptors. Furthermore, the different responses observed at early and late stages of Mo differentiation towards DCs may be relevant in mediating bLF effects in specific body districts, where these cell types may be differently represented in physiopathological conditions.http://www.mdpi.com/2072-6651/7/12/4897monocytedendritic celllactoferrinCCL1surface receptors |
| spellingShingle | Daniela Latorre Nadia Pulvirenti Daniela Angela Covino Barbara Varano Cristina Purificato Gabriella Rainaldi Maria Cristina Gauzzi Laura Fantuzzi Lucia Conti Gloria Donninelli Manuela Del Cornò Michela Sabbatucci Sandra Gessani Patrizia Puddu Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors Toxins monocyte dendritic cell lactoferrin CCL1 surface receptors |
| title | Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors |
| title_full | Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors |
| title_fullStr | Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors |
| title_full_unstemmed | Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors |
| title_short | Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors |
| title_sort | bovine lactoferrin induced ccl1 expression involves distinct receptors in monocyte derived dendritic cells and their monocyte precursors |
| topic | monocyte dendritic cell lactoferrin CCL1 surface receptors |
| url | http://www.mdpi.com/2072-6651/7/12/4897 |
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