Biological and in silico investigation of isolated novel bioactive compound from Conocarpus lancifolius
The plant Conocarpus lancifolius from Combertaceae family, has been documented to have strong medicinal potential. The objective of this research was to determine antioxidant, anti-inflammatory, and cytotoxic potential of C. lancifolius by in vitro, in vivo as well as computational studies. A foreca...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364724000338 |
| _version_ | 1797262949732057088 |
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| author | Malik Saadullah M. Fakhar-e-Alam M. Atif Muhammad Asif Kanwal Irshad Zulfiqar Ali |
| author_facet | Malik Saadullah M. Fakhar-e-Alam M. Atif Muhammad Asif Kanwal Irshad Zulfiqar Ali |
| author_sort | Malik Saadullah |
| collection | DOAJ |
| description | The plant Conocarpus lancifolius from Combertaceae family, has been documented to have strong medicinal potential. The objective of this research was to determine antioxidant, anti-inflammatory, and cytotoxic potential of C. lancifolius by in vitro, in vivo as well as computational studies. A forecast was made that a novel compound “lancifolian” can significantly improve inflammation, radical scavenging ability as well as cytotoxicity. For antioxidant study ABTS, DPPH, FRAP and β-carotene bleaching assays were performed. Anti-inflammatory activity was determined by carrageenan induced paw edema. Cytotoxic effects against four cancer cell lines: MCF-7, A549, COR-L23 and MDA-MB-231, were observed. Molecular docking was also performed to predict anti-inflammatory and cytotoxic properties by observing the binding affinity of lancifolian with respective receptors and validation of molecular docking was performed. Dichloromethane (DCM) and methanolic extract of C. lancifolius show strong radical scavenging ability. Different extracts showed a significant reduction in inflammation in the paw edema method. C. lancifolius extract and lancifolian showed significant cytotoxic effects against four cancer cell lines: MCF-7, A549, COR-L23 and MDA-MB-231. Subsequently, molecular docking highlighted the strong binding affinity of lancifolian with respective receptors. |
| first_indexed | 2024-03-08T00:50:23Z |
| format | Article |
| id | doaj.art-d174bb08e85e4e10a7fe816286f33246 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-25T00:05:14Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-d174bb08e85e4e10a7fe816286f332462024-03-14T06:13:49ZengElsevierJournal of King Saud University: Science1018-36472024-04-01364103121Biological and in silico investigation of isolated novel bioactive compound from Conocarpus lancifoliusMalik Saadullah0M. Fakhar-e-Alam1M. Atif2Muhammad Asif3Kanwal Irshad4Zulfiqar Ali5Department of Pharmaceutical Chemistry, Government College University Faisalabad, Pakistan; Corresponding author.Department of Physics, Government College University Faisalabad, PakistanDepartment of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology Faculty of Pharmacy The Islamia University BahawalpurDepartment of Pharmaceutical Chemistry, Government College University Faisalabad, PakistanDepartment of Mathematicsand Physics University of Campanialuigi Vanvitelli, Viale Abramolincolln,5, 81100 Caserta, ItalyThe plant Conocarpus lancifolius from Combertaceae family, has been documented to have strong medicinal potential. The objective of this research was to determine antioxidant, anti-inflammatory, and cytotoxic potential of C. lancifolius by in vitro, in vivo as well as computational studies. A forecast was made that a novel compound “lancifolian” can significantly improve inflammation, radical scavenging ability as well as cytotoxicity. For antioxidant study ABTS, DPPH, FRAP and β-carotene bleaching assays were performed. Anti-inflammatory activity was determined by carrageenan induced paw edema. Cytotoxic effects against four cancer cell lines: MCF-7, A549, COR-L23 and MDA-MB-231, were observed. Molecular docking was also performed to predict anti-inflammatory and cytotoxic properties by observing the binding affinity of lancifolian with respective receptors and validation of molecular docking was performed. Dichloromethane (DCM) and methanolic extract of C. lancifolius show strong radical scavenging ability. Different extracts showed a significant reduction in inflammation in the paw edema method. C. lancifolius extract and lancifolian showed significant cytotoxic effects against four cancer cell lines: MCF-7, A549, COR-L23 and MDA-MB-231. Subsequently, molecular docking highlighted the strong binding affinity of lancifolian with respective receptors.http://www.sciencedirect.com/science/article/pii/S1018364724000338AntioxidantCytotoxicLancifolianMolecular dockingConocarpus lancifoilus |
| spellingShingle | Malik Saadullah M. Fakhar-e-Alam M. Atif Muhammad Asif Kanwal Irshad Zulfiqar Ali Biological and in silico investigation of isolated novel bioactive compound from Conocarpus lancifolius Journal of King Saud University: Science Antioxidant Cytotoxic Lancifolian Molecular docking Conocarpus lancifoilus |
| title | Biological and in silico investigation of isolated novel bioactive compound from Conocarpus lancifolius |
| title_full | Biological and in silico investigation of isolated novel bioactive compound from Conocarpus lancifolius |
| title_fullStr | Biological and in silico investigation of isolated novel bioactive compound from Conocarpus lancifolius |
| title_full_unstemmed | Biological and in silico investigation of isolated novel bioactive compound from Conocarpus lancifolius |
| title_short | Biological and in silico investigation of isolated novel bioactive compound from Conocarpus lancifolius |
| title_sort | biological and in silico investigation of isolated novel bioactive compound from conocarpus lancifolius |
| topic | Antioxidant Cytotoxic Lancifolian Molecular docking Conocarpus lancifoilus |
| url | http://www.sciencedirect.com/science/article/pii/S1018364724000338 |
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