Introgression of SUB1 aggravates the susceptibility of the popular rice cultivars Swarna and Savitri to stagnant flooding
Abstract Identification of the Sub1 gene for tolerance to flash flooding and its introgression into high-yielding rice cultivars are major targets in rice breeding for flood-prone rice agro-ecosystems for ensuring yield stability. However, knowledge is scant on the response of the modified genotypes...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-35251-z |
| _version_ | 1797811575897194496 |
|---|---|
| author | Sandhya Rani Kuanar Ramani Kumar Sarkar Rashmi Panigrahi Pravat Kumar Mohapatra |
| author_facet | Sandhya Rani Kuanar Ramani Kumar Sarkar Rashmi Panigrahi Pravat Kumar Mohapatra |
| author_sort | Sandhya Rani Kuanar |
| collection | DOAJ |
| description | Abstract Identification of the Sub1 gene for tolerance to flash flooding and its introgression into high-yielding rice cultivars are major targets in rice breeding for flood-prone rice agro-ecosystems for ensuring yield stability. However, knowledge is scant on the response of the modified genotypes under stagnant flooding (SF) to meet the challenge of finding a superior allele that may confer greater resilience to the plant under a stress-prone environment. In pursuance, we have tested the response of Sub1-introgression in two popular rice varieties, Swarna and Savitri to SF by comparing the biochemical factors in the control of flag leaf senescence and its primary production mechanisms of the parental lines’ versus Sub1-introgressed lines. The activities of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GR), and ascorbate peroxidase (APX) increased while various parameters of primary production like total chlorophyll content, stomatal conductance (gs), normalized difference vegetation index (NDVI) and photosynthetic activity (Pn) decreased progressively with passage of time in the flag leaf of the cultivars during the post-anthesis period and SF-treatment increased the enzyme activity while depressing primary production further. Introgression of Sub1 had no influence on these activities under control conditions but widened the margin of effects under SF. It was concluded that the functional ability of flag leaf in mega rice cultivars like Swarna and Savitri decreased significantly by SF because of an ethylene-mediated promotion of senescence of the flag leaf. The enhancement of antioxidant enzyme activity by SF could not sustain the stability of primary production in the flag leaf. The introgression of the Sub1 gene made the cultivars more vulnerable to SF because the gene induced overexpression of ethylene. |
| first_indexed | 2024-03-13T07:25:48Z |
| format | Article |
| id | doaj.art-d175c500b2b84afc8b9b4c892fcbdd58 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T07:25:48Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d175c500b2b84afc8b9b4c892fcbdd582023-06-04T11:25:39ZengNature PortfolioScientific Reports2045-23222023-06-0113111210.1038/s41598-023-35251-zIntrogression of SUB1 aggravates the susceptibility of the popular rice cultivars Swarna and Savitri to stagnant floodingSandhya Rani Kuanar0Ramani Kumar Sarkar1Rashmi Panigrahi2Pravat Kumar Mohapatra3ICAR-National Rice Research InstituteICAR-National Rice Research InstituteSchool of Life Science, Sambalpur UniversitySchool of Life Science, Sambalpur UniversityAbstract Identification of the Sub1 gene for tolerance to flash flooding and its introgression into high-yielding rice cultivars are major targets in rice breeding for flood-prone rice agro-ecosystems for ensuring yield stability. However, knowledge is scant on the response of the modified genotypes under stagnant flooding (SF) to meet the challenge of finding a superior allele that may confer greater resilience to the plant under a stress-prone environment. In pursuance, we have tested the response of Sub1-introgression in two popular rice varieties, Swarna and Savitri to SF by comparing the biochemical factors in the control of flag leaf senescence and its primary production mechanisms of the parental lines’ versus Sub1-introgressed lines. The activities of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GR), and ascorbate peroxidase (APX) increased while various parameters of primary production like total chlorophyll content, stomatal conductance (gs), normalized difference vegetation index (NDVI) and photosynthetic activity (Pn) decreased progressively with passage of time in the flag leaf of the cultivars during the post-anthesis period and SF-treatment increased the enzyme activity while depressing primary production further. Introgression of Sub1 had no influence on these activities under control conditions but widened the margin of effects under SF. It was concluded that the functional ability of flag leaf in mega rice cultivars like Swarna and Savitri decreased significantly by SF because of an ethylene-mediated promotion of senescence of the flag leaf. The enhancement of antioxidant enzyme activity by SF could not sustain the stability of primary production in the flag leaf. The introgression of the Sub1 gene made the cultivars more vulnerable to SF because the gene induced overexpression of ethylene.https://doi.org/10.1038/s41598-023-35251-z |
| spellingShingle | Sandhya Rani Kuanar Ramani Kumar Sarkar Rashmi Panigrahi Pravat Kumar Mohapatra Introgression of SUB1 aggravates the susceptibility of the popular rice cultivars Swarna and Savitri to stagnant flooding Scientific Reports |
| title | Introgression of SUB1 aggravates the susceptibility of the popular rice cultivars Swarna and Savitri to stagnant flooding |
| title_full | Introgression of SUB1 aggravates the susceptibility of the popular rice cultivars Swarna and Savitri to stagnant flooding |
| title_fullStr | Introgression of SUB1 aggravates the susceptibility of the popular rice cultivars Swarna and Savitri to stagnant flooding |
| title_full_unstemmed | Introgression of SUB1 aggravates the susceptibility of the popular rice cultivars Swarna and Savitri to stagnant flooding |
| title_short | Introgression of SUB1 aggravates the susceptibility of the popular rice cultivars Swarna and Savitri to stagnant flooding |
| title_sort | introgression of sub1 aggravates the susceptibility of the popular rice cultivars swarna and savitri to stagnant flooding |
| url | https://doi.org/10.1038/s41598-023-35251-z |
| work_keys_str_mv | AT sandhyaranikuanar introgressionofsub1aggravatesthesusceptibilityofthepopularricecultivarsswarnaandsavitritostagnantflooding AT ramanikumarsarkar introgressionofsub1aggravatesthesusceptibilityofthepopularricecultivarsswarnaandsavitritostagnantflooding AT rashmipanigrahi introgressionofsub1aggravatesthesusceptibilityofthepopularricecultivarsswarnaandsavitritostagnantflooding AT pravatkumarmohapatra introgressionofsub1aggravatesthesusceptibilityofthepopularricecultivarsswarnaandsavitritostagnantflooding |