Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm Resources
To explore the multipurpose utilization of tobacco germplasm resources, 80 representative air/sun-cured tobacco germplasms were used as experimental materials to determine the composition and content of the diterpenoids of glandular trichome secretions using ultra-performance liquid chromatography (...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/9/2433 |
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| author | Chaofan Qi Jing Liu Yongmei Du Xiao Han Hongbo Zhang Qiujuan Fu Jianhui Zhang Yanhua Liu |
| author_facet | Chaofan Qi Jing Liu Yongmei Du Xiao Han Hongbo Zhang Qiujuan Fu Jianhui Zhang Yanhua Liu |
| author_sort | Chaofan Qi |
| collection | DOAJ |
| description | To explore the multipurpose utilization of tobacco germplasm resources, 80 representative air/sun-cured tobacco germplasms were used as experimental materials to determine the composition and content of the diterpenoids of glandular trichome secretions using ultra-performance liquid chromatography (UPLC). Correlation analysis, cluster analysis, and principal component analysis (PCA) were used to comprehensively evaluate 80 air/sun-cured tobacco germplasms. The results are as follows: (1) 11 chromatographic peaks (Peak1–Peak11) were detected by UPLC, and the coefficient of variation of their contents ranged from 26.3 to 143%. (2) Correlation analysis showed that alpha-cembratriene-diol (α-CBT-diol) and beta-cembratriene-diol (β-CBT-diol) had the highest correlation coefficients (0.97), showing a significantly negative correlation with <i>cis</i>-abienol and a significantly positive correlation with the other diterpenoids. (3) PCA showed that the cumulative contribution rate of the three principal components was 75.70%, and the diterpenoid-rich germplasms were ranked in the following order: X40 (Maiduo) > X48 (Jianpingpiaoba) > X58 (Mianzhu) > X54 (Shifangpipaliu) > X63 (Xuejia5) > X41 (Tangpeng) > X29 (OLOR) > X73 (Criollo) > X44 (Tiebanqing) > X70 (Nicaragua Changxin). The diterpenoid content of X36 was the lowest. These results provide excellent germplasm for the extraction and exploitation of the diterpenoids and for genetic studies of their metabolism. |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T23:07:56Z |
| publishDate | 2023-09-01 |
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| series | Agronomy |
| spelling | doaj.art-d179c006a5b44505a80260dac9676c932023-11-19T09:12:13ZengMDPI AGAgronomy2073-43952023-09-01139243310.3390/agronomy13092433Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm ResourcesChaofan Qi0Jing Liu1Yongmei Du2Xiao Han3Hongbo Zhang4Qiujuan Fu5Jianhui Zhang6Yanhua Liu7Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaPlant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaPlant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaPlant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaPlant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaPlant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaTobacco Science Institute of Sichuan Province, Chengdu 610094, ChinaPlant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaTo explore the multipurpose utilization of tobacco germplasm resources, 80 representative air/sun-cured tobacco germplasms were used as experimental materials to determine the composition and content of the diterpenoids of glandular trichome secretions using ultra-performance liquid chromatography (UPLC). Correlation analysis, cluster analysis, and principal component analysis (PCA) were used to comprehensively evaluate 80 air/sun-cured tobacco germplasms. The results are as follows: (1) 11 chromatographic peaks (Peak1–Peak11) were detected by UPLC, and the coefficient of variation of their contents ranged from 26.3 to 143%. (2) Correlation analysis showed that alpha-cembratriene-diol (α-CBT-diol) and beta-cembratriene-diol (β-CBT-diol) had the highest correlation coefficients (0.97), showing a significantly negative correlation with <i>cis</i>-abienol and a significantly positive correlation with the other diterpenoids. (3) PCA showed that the cumulative contribution rate of the three principal components was 75.70%, and the diterpenoid-rich germplasms were ranked in the following order: X40 (Maiduo) > X48 (Jianpingpiaoba) > X58 (Mianzhu) > X54 (Shifangpipaliu) > X63 (Xuejia5) > X41 (Tangpeng) > X29 (OLOR) > X73 (Criollo) > X44 (Tiebanqing) > X70 (Nicaragua Changxin). The diterpenoid content of X36 was the lowest. These results provide excellent germplasm for the extraction and exploitation of the diterpenoids and for genetic studies of their metabolism.https://www.mdpi.com/2073-4395/13/9/2433air/sun-cured tobacco germplasm resourcesglandular trichome secretionditerpenoidsidentification and evaluation |
| spellingShingle | Chaofan Qi Jing Liu Yongmei Du Xiao Han Hongbo Zhang Qiujuan Fu Jianhui Zhang Yanhua Liu Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm Resources Agronomy air/sun-cured tobacco germplasm resources glandular trichome secretion diterpenoids identification and evaluation |
| title | Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm Resources |
| title_full | Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm Resources |
| title_fullStr | Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm Resources |
| title_full_unstemmed | Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm Resources |
| title_short | Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm Resources |
| title_sort | identification and evaluation of diterpenoids from glandular trichome secretions of air sun cured tobacco germplasm resources |
| topic | air/sun-cured tobacco germplasm resources glandular trichome secretion diterpenoids identification and evaluation |
| url | https://www.mdpi.com/2073-4395/13/9/2433 |
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