Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples
Salmonellosis is a common infectious disease in humans caused by Salmonella spp., which in recent years has shown an increase in its incidence, with products of avian origin being a common source of transmission. To present a successful infective cycle, there are molecular mechanisms such as virulen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2023-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2023/1875253 |
| _version_ | 1797871888549019648 |
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| author | Kelly Johanna Lozano-Villegas María Paula Herrera-Sánchez Mónica Alexandra Beltrán-Martínez Stefany Cárdenas-Moscoso Iang Schroniltgen Rondón-Barragán |
| author_facet | Kelly Johanna Lozano-Villegas María Paula Herrera-Sánchez Mónica Alexandra Beltrán-Martínez Stefany Cárdenas-Moscoso Iang Schroniltgen Rondón-Barragán |
| author_sort | Kelly Johanna Lozano-Villegas |
| collection | DOAJ |
| description | Salmonellosis is a common infectious disease in humans caused by Salmonella spp., which in recent years has shown an increase in its incidence, with products of avian origin being a common source of transmission. To present a successful infective cycle, there are molecular mechanisms such as virulence factors that provide characteristics that facilitate survival, colonization, and damage to the host. According to this, the study aims to characterize the virulence factors of Salmonella spp. strains isolated from broilers (n = 39) and humans (n = 10). The presence of 24 virulence genes was evaluated using end-point PCR. All the strains of Salmonella spp. isolated from broiler chickens revealed presence of 7/24 (29, 16%) virulence genes (lpfA, csgA, sitC, sipB, sopB, sopE, and sivH). Regarding the strains isolated from cases of gastroenteritis in humans, all strains contained (14/24, 58, 33%) virulence genes (lpfA, csgA, pagC, msgA, spiA, sitC, iroN, sipB, orgA, hilA, sopB, sifA, avrA, and sivH). In summary, the presence of virulence genes in different strains of Salmonella isolated from broilers and humans could be described as bacteria with potential pathogenicity due to the type and number of virulence genes detected. These findings are beneficial for the pathogenic monitoring of Salmonella in Colombia. |
| first_indexed | 2024-04-10T00:50:08Z |
| format | Article |
| id | doaj.art-d17ceceb73984eadb39639fe9606b3b2 |
| institution | Directory Open Access Journal |
| issn | 2042-0048 |
| language | English |
| last_indexed | 2024-04-10T00:50:08Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj.art-d17ceceb73984eadb39639fe9606b3b22023-03-13T11:25:53ZengHindawi LimitedVeterinary Medicine International2042-00482023-01-01202310.1155/2023/1875253Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human SamplesKelly Johanna Lozano-Villegas0María Paula Herrera-Sánchez1Mónica Alexandra Beltrán-Martínez2Stefany Cárdenas-Moscoso3Iang Schroniltgen Rondón-Barragán4Poultry Research GroupPoultry Research GroupImmunobiology and Pathogenesis Research GroupImmunobiology and Pathogenesis Research GroupPoultry Research GroupSalmonellosis is a common infectious disease in humans caused by Salmonella spp., which in recent years has shown an increase in its incidence, with products of avian origin being a common source of transmission. To present a successful infective cycle, there are molecular mechanisms such as virulence factors that provide characteristics that facilitate survival, colonization, and damage to the host. According to this, the study aims to characterize the virulence factors of Salmonella spp. strains isolated from broilers (n = 39) and humans (n = 10). The presence of 24 virulence genes was evaluated using end-point PCR. All the strains of Salmonella spp. isolated from broiler chickens revealed presence of 7/24 (29, 16%) virulence genes (lpfA, csgA, sitC, sipB, sopB, sopE, and sivH). Regarding the strains isolated from cases of gastroenteritis in humans, all strains contained (14/24, 58, 33%) virulence genes (lpfA, csgA, pagC, msgA, spiA, sitC, iroN, sipB, orgA, hilA, sopB, sifA, avrA, and sivH). In summary, the presence of virulence genes in different strains of Salmonella isolated from broilers and humans could be described as bacteria with potential pathogenicity due to the type and number of virulence genes detected. These findings are beneficial for the pathogenic monitoring of Salmonella in Colombia.http://dx.doi.org/10.1155/2023/1875253 |
| spellingShingle | Kelly Johanna Lozano-Villegas María Paula Herrera-Sánchez Mónica Alexandra Beltrán-Martínez Stefany Cárdenas-Moscoso Iang Schroniltgen Rondón-Barragán Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples Veterinary Medicine International |
| title | Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples |
| title_full | Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples |
| title_fullStr | Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples |
| title_full_unstemmed | Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples |
| title_short | Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples |
| title_sort | molecular detection of virulence factors in salmonella serovars isolated from poultry and human samples |
| url | http://dx.doi.org/10.1155/2023/1875253 |
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