Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins
A rapid and simple capillary electrophoresis method has been developed for the simultaneous determination of six extensively used cephalosporin antibiotics (cefaclor, cefadroxil, cefalexin, cefuroxim, ceftazidim, ceftriaxon). The determination of cephalosporins was performed at a pH 6.8, using a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2013-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2013/0352-51391300028H.pdf |
| _version_ | 1819026069398749184 |
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| author | Hancu Gabriel Kelemen Hajnal Rusu Aura Gyéresi Árpád |
| author_facet | Hancu Gabriel Kelemen Hajnal Rusu Aura Gyéresi Árpád |
| author_sort | Hancu Gabriel |
| collection | DOAJ |
| description | A rapid and simple capillary electrophoresis method has been developed for the simultaneous determination of six extensively used cephalosporin antibiotics (cefaclor, cefadroxil, cefalexin, cefuroxim, ceftazidim, ceftriaxon). The determination of cephalosporins was performed at a pH 6.8, using a 25 mM phospate - 25 mM borate mixed buffer, + 25 kV voltage at a temperature of 25 °C. We achieved a baseline separation in approximately 10 minutes. The separation resolution was increased by addition of an anionic surfactant, 50 mM sodium dodecyl sulfate, to the buffer solution. The proposed separation was evaluated on the basis of detection and quantification limits, effective electrophoretic mobility and relative standard deviation for migration times and peak areas. |
| first_indexed | 2024-12-21T05:20:43Z |
| format | Article |
| id | doaj.art-d17d41bf6b9d4630be1209363b635c15 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 |
| language | English |
| last_indexed | 2024-12-21T05:20:43Z |
| publishDate | 2013-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-d17d41bf6b9d4630be1209363b635c152022-12-21T19:14:49ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392013-01-017891413142310.2298/JSC121117028HDevelopment of a capillary electrophoresis method for the simultaneous determination of cephalosporinsHancu GabrielKelemen HajnalRusu AuraGyéresi ÁrpádA rapid and simple capillary electrophoresis method has been developed for the simultaneous determination of six extensively used cephalosporin antibiotics (cefaclor, cefadroxil, cefalexin, cefuroxim, ceftazidim, ceftriaxon). The determination of cephalosporins was performed at a pH 6.8, using a 25 mM phospate - 25 mM borate mixed buffer, + 25 kV voltage at a temperature of 25 °C. We achieved a baseline separation in approximately 10 minutes. The separation resolution was increased by addition of an anionic surfactant, 50 mM sodium dodecyl sulfate, to the buffer solution. The proposed separation was evaluated on the basis of detection and quantification limits, effective electrophoretic mobility and relative standard deviation for migration times and peak areas.http://www.doiserbia.nb.rs/img/doi/0352-5139/2013/0352-51391300028H.pdfcephalosporinscapillary zone electrophoresismicellar electro-kinetic chromatography |
| spellingShingle | Hancu Gabriel Kelemen Hajnal Rusu Aura Gyéresi Árpád Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins Journal of the Serbian Chemical Society cephalosporins capillary zone electrophoresis micellar electro-kinetic chromatography |
| title | Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins |
| title_full | Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins |
| title_fullStr | Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins |
| title_full_unstemmed | Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins |
| title_short | Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins |
| title_sort | development of a capillary electrophoresis method for the simultaneous determination of cephalosporins |
| topic | cephalosporins capillary zone electrophoresis micellar electro-kinetic chromatography |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2013/0352-51391300028H.pdf |
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