Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways
Salinity stress is one of the serious factors, limiting production of major agricultural crops; especially, in sodic soils. A number of approaches are being applied to mitigate the salt-induced adverse effects in agricultural crops through implying different halotolerant microbes. In this aspect, a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00423/full |
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| author | Vikas K. Patel Ruchi Srivastava Anjney Sharma Anchal K. Srivastava Savita Singh Alok K. Srivastava Prem L. Kashyap Hillol Chakdar K. Pandiyan Alok Kalra Anil K. Saxena |
| author_facet | Vikas K. Patel Ruchi Srivastava Anjney Sharma Anchal K. Srivastava Savita Singh Alok K. Srivastava Prem L. Kashyap Hillol Chakdar K. Pandiyan Alok Kalra Anil K. Saxena |
| author_sort | Vikas K. Patel |
| collection | DOAJ |
| description | Salinity stress is one of the serious factors, limiting production of major agricultural crops; especially, in sodic soils. A number of approaches are being applied to mitigate the salt-induced adverse effects in agricultural crops through implying different halotolerant microbes. In this aspect, a halotolerant, Exiguobacterium profundum PHM11 was evaluated under eight different salinity regimes; 100, 250, 500, 1000, 1500, 2000, 2500, and 3000 mM to know its inherent salt tolerance limits and salt-induced consequences affecting its natural metabolism. Based on the stoichiometric growth kinetics; 100 and 1500 mM concentrations were selected as optimal and minimal performance limits for PHM11. To know, how salt stress affects the expression profiles of regulatory genes of its key metabolic pathways, and total production of important metabolites; biomass, carotenoids, beta-carotene production, IAA and proline contents, and expression profiles of key genes affecting the protein folding, structural adaptations, transportation across the cell membrane, stress tolerance, carotenoids, IAA and mannitol production in PHM11 were studied under 100 and 1500 mM salinity. E. profundum PHM11 showed maximum and minimum growth, biomass and metabolite production at 100 and 1500 mM salinity respectively. Salt-induced fine-tuning of expression profiles of key genes of stress pathways was determined in halotolerant bacterium PHM11. |
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| language | English |
| last_indexed | 2024-12-16T08:29:32Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-d17fc2c99c5e4955874eda69b3270d852022-12-21T22:37:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-03-01910.3389/fmicb.2018.00423318503Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic PathwaysVikas K. Patel0Ruchi Srivastava1Anjney Sharma2Anchal K. Srivastava3Savita Singh4Alok K. Srivastava5Prem L. Kashyap6Hillol Chakdar7K. Pandiyan8Alok Kalra9Anil K. Saxena10Laboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaLaboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaLaboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaLaboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaLaboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaLaboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaDivision of Plant Pathology, ICAR-Indian Institute of Wheat and Barley Research, Karnal, IndiaLaboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaLaboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaDepartment of Microbial Technology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, IndiaLaboratory of Genomics, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, IndiaSalinity stress is one of the serious factors, limiting production of major agricultural crops; especially, in sodic soils. A number of approaches are being applied to mitigate the salt-induced adverse effects in agricultural crops through implying different halotolerant microbes. In this aspect, a halotolerant, Exiguobacterium profundum PHM11 was evaluated under eight different salinity regimes; 100, 250, 500, 1000, 1500, 2000, 2500, and 3000 mM to know its inherent salt tolerance limits and salt-induced consequences affecting its natural metabolism. Based on the stoichiometric growth kinetics; 100 and 1500 mM concentrations were selected as optimal and minimal performance limits for PHM11. To know, how salt stress affects the expression profiles of regulatory genes of its key metabolic pathways, and total production of important metabolites; biomass, carotenoids, beta-carotene production, IAA and proline contents, and expression profiles of key genes affecting the protein folding, structural adaptations, transportation across the cell membrane, stress tolerance, carotenoids, IAA and mannitol production in PHM11 were studied under 100 and 1500 mM salinity. E. profundum PHM11 showed maximum and minimum growth, biomass and metabolite production at 100 and 1500 mM salinity respectively. Salt-induced fine-tuning of expression profiles of key genes of stress pathways was determined in halotolerant bacterium PHM11.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00423/fullExiguobacterium profundum PHM11salinitygene expressionmetabolic pathwaysbeta-carotene |
| spellingShingle | Vikas K. Patel Ruchi Srivastava Anjney Sharma Anchal K. Srivastava Savita Singh Alok K. Srivastava Prem L. Kashyap Hillol Chakdar K. Pandiyan Alok Kalra Anil K. Saxena Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways Frontiers in Microbiology Exiguobacterium profundum PHM11 salinity gene expression metabolic pathways beta-carotene |
| title | Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways |
| title_full | Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways |
| title_fullStr | Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways |
| title_full_unstemmed | Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways |
| title_short | Halotolerant Exiguobacterium profundum PHM11 Tolerate Salinity by Accumulating L-Proline and Fine-Tuning Gene Expression Profiles of Related Metabolic Pathways |
| title_sort | halotolerant exiguobacterium profundum phm11 tolerate salinity by accumulating l proline and fine tuning gene expression profiles of related metabolic pathways |
| topic | Exiguobacterium profundum PHM11 salinity gene expression metabolic pathways beta-carotene |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00423/full |
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