Anti-Helicobacter pylori potential, antioxidant capacity, and anti-inflammatory activity of Xylopia sericea A. St.-Hil. (Annonaceae) leaves
Background: : Xylopia sericea A. St.-Hil. (Annonaceae), a Brazilian pepper, popularly known as “pindaíba,” has ethnopharmacological relevance as a useful remedy for gastric disorders; however, few reports are available in the literature to explain its therapeutic effects. Purpose: : This study aimed...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031321001962 |
| _version_ | 1818870004373782528 |
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| author | João Victor Dutra Gomes Augusto Santos Borges Brena Ramos Athaydes Kicia Karinne Pereira Gomes-Copeland Damaris Silveira Pérola Oliveira Magalhães Rita de Cássia Ribeiro Gonçalves Rodrigo Rezende Kitagawa Yris Maria Fonseca-Bazzo Claudia Masrouah Jamal |
| author_facet | João Victor Dutra Gomes Augusto Santos Borges Brena Ramos Athaydes Kicia Karinne Pereira Gomes-Copeland Damaris Silveira Pérola Oliveira Magalhães Rita de Cássia Ribeiro Gonçalves Rodrigo Rezende Kitagawa Yris Maria Fonseca-Bazzo Claudia Masrouah Jamal |
| author_sort | João Victor Dutra Gomes |
| collection | DOAJ |
| description | Background: : Xylopia sericea A. St.-Hil. (Annonaceae), a Brazilian pepper, popularly known as “pindaíba,” has ethnopharmacological relevance as a useful remedy for gastric disorders; however, few reports are available in the literature to explain its therapeutic effects. Purpose: : This study aimed to evaluate the in vitro effects of X. sericea leaf ethanol extract (EE) on Helicobacter pylori (HP) growth, cytokine production, antioxidant capacity, and cytotoxicity in human gastric adenocarcinoma cell line (AGS). Study design: : Using a cell model system (murine macrophage LPS-stimulated) and in vitro assays, EE bioactivity, with regard to antioxidant capacity, inhibition of cytokine production, and urease inhibition, was assessed. The EE chemical profile was obtained using chromatography and biomarker quantification. Methods: : Antioxidant and urease assays were performed using colorimetric methods, H. pylori growth was evaluated using the minimal inhibitory concentration assay, cytokine production was measured using enzyme-linked immunosorbent assay, cytotoxicity was determined using the MTT method, EE chromatographic profile was obtained using HPLC-DAD-UV analysis, and biomarker quantification was obtained using calibration curves. Results: : Our findings demonstrated that EE has an antioxidant capacity similar to that of gallic acid in the hypochlorous acid inhibition assay (EC50 = 61.09 µg/ml) and higher activity (p <0.05) in the superoxide radical assay (EC50 = 25.56 µg/ml) compared to the same antioxidant standard. In RAW 264.7 LPS stimulated cells, EE (100 µg/ml) inhibited the production of NO (76%) and IL-6 (85%) but did not reduce TNF-α and superoxide levels at all tested concentrations (6.25 to 100 µg/ml). EE demonstrated urease enzyme inhibition similar to boric acid, the standard inhibitor, and inhibited H. pylori non-resistant strain growth at 512 µg/mL. EE did not reduce the AGS cell viability at the tested concentrations (6.25 to 100 µg/ml). The EE flavonoid content was 198.50 µg of rutin equivalents in each mg of extract, and the HPLC analyses showed four majoritarian components as quercetin glycosides. Conclusions: : This study partially explains the role of X. sericea in defense mechanisms associated with gastric diseases, and provides support for further research on the potential of X. sericea in gastric disease treatments. |
| first_indexed | 2024-12-19T12:00:08Z |
| format | Article |
| id | doaj.art-d180b379b3de402ea3b54be467cf93a3 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-12-19T12:00:08Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-d180b379b3de402ea3b54be467cf93a32022-12-21T20:22:31ZengElsevierPhytomedicine Plus2667-03132022-02-0121100214Anti-Helicobacter pylori potential, antioxidant capacity, and anti-inflammatory activity of Xylopia sericea A. St.-Hil. (Annonaceae) leavesJoão Victor Dutra Gomes0Augusto Santos Borges1Brena Ramos Athaydes2Kicia Karinne Pereira Gomes-Copeland3Damaris Silveira4Pérola Oliveira Magalhães5Rita de Cássia Ribeiro Gonçalves6Rodrigo Rezende Kitagawa7Yris Maria Fonseca-Bazzo8Claudia Masrouah Jamal9Health Sciences College, University of Brasília, Brasília, 70910-900, Brazil; Corresponding author at: Laboratory of Natural Products, Health Sciences College, University of Brasília. Asa Norte, Brasilia, Distrito Federal, CEP 70910-900 BrazilDepartment of Pharmacy, State University of Maringá, 87020-900, BrazilDepartment of Pharmaceutical Sciences, Federal University of Espírito Santo, Vitória, 29043-900, BrazilHealth Sciences College, University of Brasília, Brasília, 70910-900, BrazilHealth Sciences College, University of Brasília, Brasília, 70910-900, BrazilHealth Sciences College, University of Brasília, Brasília, 70910-900, BrazilDepartment of Pharmaceutical Sciences, Federal University of Espírito Santo, Vitória, 29043-900, BrazilDepartment of Pharmaceutical Sciences, Federal University of Espírito Santo, Vitória, 29043-900, BrazilHealth Sciences College, University of Brasília, Brasília, 70910-900, BrazilDepartment of Pharmaceutical Sciences, Federal University of Espírito Santo, Vitória, 29043-900, BrazilBackground: : Xylopia sericea A. St.-Hil. (Annonaceae), a Brazilian pepper, popularly known as “pindaíba,” has ethnopharmacological relevance as a useful remedy for gastric disorders; however, few reports are available in the literature to explain its therapeutic effects. Purpose: : This study aimed to evaluate the in vitro effects of X. sericea leaf ethanol extract (EE) on Helicobacter pylori (HP) growth, cytokine production, antioxidant capacity, and cytotoxicity in human gastric adenocarcinoma cell line (AGS). Study design: : Using a cell model system (murine macrophage LPS-stimulated) and in vitro assays, EE bioactivity, with regard to antioxidant capacity, inhibition of cytokine production, and urease inhibition, was assessed. The EE chemical profile was obtained using chromatography and biomarker quantification. Methods: : Antioxidant and urease assays were performed using colorimetric methods, H. pylori growth was evaluated using the minimal inhibitory concentration assay, cytokine production was measured using enzyme-linked immunosorbent assay, cytotoxicity was determined using the MTT method, EE chromatographic profile was obtained using HPLC-DAD-UV analysis, and biomarker quantification was obtained using calibration curves. Results: : Our findings demonstrated that EE has an antioxidant capacity similar to that of gallic acid in the hypochlorous acid inhibition assay (EC50 = 61.09 µg/ml) and higher activity (p <0.05) in the superoxide radical assay (EC50 = 25.56 µg/ml) compared to the same antioxidant standard. In RAW 264.7 LPS stimulated cells, EE (100 µg/ml) inhibited the production of NO (76%) and IL-6 (85%) but did not reduce TNF-α and superoxide levels at all tested concentrations (6.25 to 100 µg/ml). EE demonstrated urease enzyme inhibition similar to boric acid, the standard inhibitor, and inhibited H. pylori non-resistant strain growth at 512 µg/mL. EE did not reduce the AGS cell viability at the tested concentrations (6.25 to 100 µg/ml). The EE flavonoid content was 198.50 µg of rutin equivalents in each mg of extract, and the HPLC analyses showed four majoritarian components as quercetin glycosides. Conclusions: : This study partially explains the role of X. sericea in defense mechanisms associated with gastric diseases, and provides support for further research on the potential of X. sericea in gastric disease treatments.http://www.sciencedirect.com/science/article/pii/S2667031321001962Quercetin glycosidesAntioxidant capacityHelicobacter pyloriAnti-inflammatory activityUrease enzyme inhibition |
| spellingShingle | João Victor Dutra Gomes Augusto Santos Borges Brena Ramos Athaydes Kicia Karinne Pereira Gomes-Copeland Damaris Silveira Pérola Oliveira Magalhães Rita de Cássia Ribeiro Gonçalves Rodrigo Rezende Kitagawa Yris Maria Fonseca-Bazzo Claudia Masrouah Jamal Anti-Helicobacter pylori potential, antioxidant capacity, and anti-inflammatory activity of Xylopia sericea A. St.-Hil. (Annonaceae) leaves Phytomedicine Plus Quercetin glycosides Antioxidant capacity Helicobacter pylori Anti-inflammatory activity Urease enzyme inhibition |
| title | Anti-Helicobacter pylori potential, antioxidant capacity, and anti-inflammatory activity of Xylopia sericea A. St.-Hil. (Annonaceae) leaves |
| title_full | Anti-Helicobacter pylori potential, antioxidant capacity, and anti-inflammatory activity of Xylopia sericea A. St.-Hil. (Annonaceae) leaves |
| title_fullStr | Anti-Helicobacter pylori potential, antioxidant capacity, and anti-inflammatory activity of Xylopia sericea A. St.-Hil. (Annonaceae) leaves |
| title_full_unstemmed | Anti-Helicobacter pylori potential, antioxidant capacity, and anti-inflammatory activity of Xylopia sericea A. St.-Hil. (Annonaceae) leaves |
| title_short | Anti-Helicobacter pylori potential, antioxidant capacity, and anti-inflammatory activity of Xylopia sericea A. St.-Hil. (Annonaceae) leaves |
| title_sort | anti helicobacter pylori potential antioxidant capacity and anti inflammatory activity of xylopia sericea a st hil annonaceae leaves |
| topic | Quercetin glycosides Antioxidant capacity Helicobacter pylori Anti-inflammatory activity Urease enzyme inhibition |
| url | http://www.sciencedirect.com/science/article/pii/S2667031321001962 |
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