Expression of the rice hoja blanca virus (RHBV) non-structural protein 3 (NS3) in Escherichia coli and its in situ localization in RHBV-infected rice tissues
The non-structural NS3 protein gene from the rice hoja blanca virus (RHBV) was fused to the glutathione- S-transferase carboxilic end and expressed in Escherichia coli strain JM83. Large quantities of fusion protein were produced in insoluble form. The fusion protein was fractionated in SDS-PAGE and...
Main Authors: | Miguel Muñoz, Isela Bolaños, Griselda Arrieta-Espinoza, Ana M Espinoza |
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Format: | Article |
Language: | English |
Published: |
Vicerractoría Investigación
2004-09-01
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Series: | Revista de Biología Tropical |
Subjects: | |
Online Access: | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442004000300037 |
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