Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonates

Background and Objective: Low electromagnetic fields (LEMF) are produced by instruments which are works with electricity. This study was done to determine the effect of LEMF on fetal death and bone marrow megakaryocytes in NMRI mouse neonates. Materials and Methods: In this experimental study 64 fem...

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Main Authors: Parvin Dokht Bayat (PhD), Mohammad Reza Darabi (PhD)
Format: Article
Language:fas
Published: Golestan University of Medical Sciences 2009-09-01
Series:مجله دانشگاه علوم پزشکی گرگان
Subjects:
Online Access:http://goums.ac.ir/journal/browse.php?a_code=A-10-1-393&slc_lang=en&sid=1
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author Parvin Dokht Bayat (PhD)
Mohammad Reza Darabi (PhD)
author_facet Parvin Dokht Bayat (PhD)
Mohammad Reza Darabi (PhD)
author_sort Parvin Dokht Bayat (PhD)
collection DOAJ
description Background and Objective: Low electromagnetic fields (LEMF) are produced by instruments which are works with electricity. This study was done to determine the effect of LEMF on fetal death and bone marrow megakaryocytes in NMRI mouse neonates. Materials and Methods: In this experimental study 64 females’ mice with 6-8 old weeks were used. 2 female mice coupled with one male, and positive vaginal plaque was interpreted as the zero day of pregnancy (GD=0). The pregnant mice were randomly categorized into control and experimental groups. The experimental group were exposed to50HZ, 0.5 mT Low electromagnetic fields on 7-11 days of pregnant period (8h/d). The weight of neonate and death fetus were studied after delivery. The live neonates were dissected on 15th day, and 1 ml of bone marrow was extracted from Tibia and vertebral column, by pressing method. The bone marrow cells suspended in 1:1 IMDM in 15cc (FULCON) tubule and cells was counted with neobar lam. The data were tested by t-student test significance was set up at p<0.05. Results: There was significant differences between the mean weight of one day neonate in cases with controls (P<0.05). The mean of dead fetus in experimental group was higher than controls (P<0.05). The mean of megakaryocytes numbers higher than controls, but this differences was not significant. Conclusion: This study showed that the number of megakaryocytes and fetal death were increased by low electromagnetic fields exposure during pregnancy.
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spelling doaj.art-d183153fbcac46f9bc5f458a54e016d12022-12-22T01:44:56ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802009-09-01113812Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonatesParvin Dokht Bayat (PhD)0Mohammad Reza Darabi (PhD)1 Background and Objective: Low electromagnetic fields (LEMF) are produced by instruments which are works with electricity. This study was done to determine the effect of LEMF on fetal death and bone marrow megakaryocytes in NMRI mouse neonates. Materials and Methods: In this experimental study 64 females’ mice with 6-8 old weeks were used. 2 female mice coupled with one male, and positive vaginal plaque was interpreted as the zero day of pregnancy (GD=0). The pregnant mice were randomly categorized into control and experimental groups. The experimental group were exposed to50HZ, 0.5 mT Low electromagnetic fields on 7-11 days of pregnant period (8h/d). The weight of neonate and death fetus were studied after delivery. The live neonates were dissected on 15th day, and 1 ml of bone marrow was extracted from Tibia and vertebral column, by pressing method. The bone marrow cells suspended in 1:1 IMDM in 15cc (FULCON) tubule and cells was counted with neobar lam. The data were tested by t-student test significance was set up at p<0.05. Results: There was significant differences between the mean weight of one day neonate in cases with controls (P<0.05). The mean of dead fetus in experimental group was higher than controls (P<0.05). The mean of megakaryocytes numbers higher than controls, but this differences was not significant. Conclusion: This study showed that the number of megakaryocytes and fetal death were increased by low electromagnetic fields exposure during pregnancy.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-393&slc_lang=en&sid=1Low electromagnetic fieldsMegakaryocytesBone marrowDead fetusMouse
spellingShingle Parvin Dokht Bayat (PhD)
Mohammad Reza Darabi (PhD)
Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonates
مجله دانشگاه علوم پزشکی گرگان
Low electromagnetic fields
Megakaryocytes
Bone marrow
Dead fetus
Mouse
title Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonates
title_full Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonates
title_fullStr Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonates
title_full_unstemmed Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonates
title_short Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonates
title_sort effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in nmri mouse neonates
topic Low electromagnetic fields
Megakaryocytes
Bone marrow
Dead fetus
Mouse
url http://goums.ac.ir/journal/browse.php?a_code=A-10-1-393&slc_lang=en&sid=1
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