Optimization of Initial pH and Total Sugar Concentration Variables on Citric Acid Production from Pineapple Waste with Aspergillus niger Yeast by Using Response Surface Methodology
Citric acid can be produced from pineapple waste by using fermentation process. This process is done in bubble column reactor with Aspergillus niger yeast. The objective of this research is to find the optimum conditions of initial pH and total sugar concentration. The optimization method used was r...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Syiah Kuala, Chemical Engineering Department
2011-06-01
|
| Series: | Jurnal Rekayasa Kimia & Lingkungan |
| Online Access: | https://jurnal.usk.ac.id/RKL/article/view/241 |
| _version_ | 1797230149244026880 |
|---|---|
| author | Widayat Widayat Abdullah Abdullah H. Satriadi M. Hadi |
| author_facet | Widayat Widayat Abdullah Abdullah H. Satriadi M. Hadi |
| author_sort | Widayat Widayat |
| collection | DOAJ |
| description | Citric acid can be produced from pineapple waste by using fermentation process. This process is done in bubble column reactor with Aspergillus niger yeast. The objective of this research is to find the optimum conditions of initial pH and total sugar concentration. The optimization method used was response surface methodology. This research was carried out at a temperature of 30 oC, spore concentration of 1.23 x 109 spore/ml, total volume 2.0 liter, flow rate of air 58.07 cc/sec and a 5% antifoam concentration. The fermentation process lasted 7 days and the citric acid concentration was analyzed by High Pressure Liquid Cromatography (HPLC) method. Statistica 6 software was used for the data treatment. The mathematical model for the optimization citric acid fermentation in bubble column reactor is Y = 54.507 + 2.9851X - 8.987X12 - 2.581X2 - 15.446X22 - 7.989X1X2 The parameter of Y is citric acid yield, X1 is a coding initial pH and X2 is a coding total sugar concentration. The results has given an initial pH optimum 3.61 and total sugar concentration 19,285% w/v with optimum an yield of 55.03 % .
Keywords: Bubble column bioreactor, Citric acid fermentation, Initial pH, Total sugar concentration, Response surface methodology |
| first_indexed | 2024-03-13T00:15:51Z |
| format | Article |
| id | doaj.art-d186325b48914fb19b477241bae24712 |
| institution | Directory Open Access Journal |
| issn | 1412-5064 2356-1661 |
| language | English |
| last_indexed | 2024-04-24T15:23:53Z |
| publishDate | 2011-06-01 |
| publisher | Universitas Syiah Kuala, Chemical Engineering Department |
| record_format | Article |
| series | Jurnal Rekayasa Kimia & Lingkungan |
| spelling | doaj.art-d186325b48914fb19b477241bae247122024-04-02T07:11:41ZengUniversitas Syiah Kuala, Chemical Engineering DepartmentJurnal Rekayasa Kimia & Lingkungan1412-50642356-16612011-06-0181236Optimization of Initial pH and Total Sugar Concentration Variables on Citric Acid Production from Pineapple Waste with Aspergillus niger Yeast by Using Response Surface MethodologyWidayat Widayat0Abdullah Abdullah1H. Satriadi2M. Hadi3Department of Chemical Engineering, Faculty of Engineering Diponegoro UniversityDepartment of Chemical Engineering, Faculty of Engineering Diponegoro UniversityDepartment of Chemical Engineering, Faculty of Engineering Diponegoro UniversityDepartment of Biology, Faculty of Mathematic and Science, Diponegoro UniversityCitric acid can be produced from pineapple waste by using fermentation process. This process is done in bubble column reactor with Aspergillus niger yeast. The objective of this research is to find the optimum conditions of initial pH and total sugar concentration. The optimization method used was response surface methodology. This research was carried out at a temperature of 30 oC, spore concentration of 1.23 x 109 spore/ml, total volume 2.0 liter, flow rate of air 58.07 cc/sec and a 5% antifoam concentration. The fermentation process lasted 7 days and the citric acid concentration was analyzed by High Pressure Liquid Cromatography (HPLC) method. Statistica 6 software was used for the data treatment. The mathematical model for the optimization citric acid fermentation in bubble column reactor is Y = 54.507 + 2.9851X - 8.987X12 - 2.581X2 - 15.446X22 - 7.989X1X2 The parameter of Y is citric acid yield, X1 is a coding initial pH and X2 is a coding total sugar concentration. The results has given an initial pH optimum 3.61 and total sugar concentration 19,285% w/v with optimum an yield of 55.03 % . Keywords: Bubble column bioreactor, Citric acid fermentation, Initial pH, Total sugar concentration, Response surface methodologyhttps://jurnal.usk.ac.id/RKL/article/view/241 |
| spellingShingle | Widayat Widayat Abdullah Abdullah H. Satriadi M. Hadi Optimization of Initial pH and Total Sugar Concentration Variables on Citric Acid Production from Pineapple Waste with Aspergillus niger Yeast by Using Response Surface Methodology Jurnal Rekayasa Kimia & Lingkungan |
| title | Optimization of Initial pH and Total Sugar Concentration Variables on Citric Acid Production from Pineapple Waste with Aspergillus niger Yeast by Using Response Surface Methodology |
| title_full | Optimization of Initial pH and Total Sugar Concentration Variables on Citric Acid Production from Pineapple Waste with Aspergillus niger Yeast by Using Response Surface Methodology |
| title_fullStr | Optimization of Initial pH and Total Sugar Concentration Variables on Citric Acid Production from Pineapple Waste with Aspergillus niger Yeast by Using Response Surface Methodology |
| title_full_unstemmed | Optimization of Initial pH and Total Sugar Concentration Variables on Citric Acid Production from Pineapple Waste with Aspergillus niger Yeast by Using Response Surface Methodology |
| title_short | Optimization of Initial pH and Total Sugar Concentration Variables on Citric Acid Production from Pineapple Waste with Aspergillus niger Yeast by Using Response Surface Methodology |
| title_sort | optimization of initial ph and total sugar concentration variables on citric acid production from pineapple waste with aspergillus niger yeast by using response surface methodology |
| url | https://jurnal.usk.ac.id/RKL/article/view/241 |
| work_keys_str_mv | AT widayatwidayat optimizationofinitialphandtotalsugarconcentrationvariablesoncitricacidproductionfrompineapplewastewithaspergillusnigeryeastbyusingresponsesurfacemethodology AT abdullahabdullah optimizationofinitialphandtotalsugarconcentrationvariablesoncitricacidproductionfrompineapplewastewithaspergillusnigeryeastbyusingresponsesurfacemethodology AT hsatriadi optimizationofinitialphandtotalsugarconcentrationvariablesoncitricacidproductionfrompineapplewastewithaspergillusnigeryeastbyusingresponsesurfacemethodology AT mhadi optimizationofinitialphandtotalsugarconcentrationvariablesoncitricacidproductionfrompineapplewastewithaspergillusnigeryeastbyusingresponsesurfacemethodology |